RelA/NFkB p65 Antibody - BSA Free

Immunoprecipitation: RelA/NFkB p65 Antibody [NB100-97831] - Detection of Human RelA/p65 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RelA/p65 antibody NB100-97831 used for IP at 3 mcg/mg lysate. RelA/p65 was also immunoprecipitated by rabbit anti-RelA/p65 antibody NB100-97832, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RelA/NFkB p65 Antibody - BSA Free Summary

• Description: This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-1803, NB100-224, NB100-376, NB100-464, NB500-160, NB100-381, NB200-347, NB100-2241, NBP1-06527
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human reticuloendotheliosis viral oncogene homolog A using the numbering given in entry NP_068810.2 (GeneID 5970).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RELA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: Not recommended for western blot.
• Control:

  RelA/NFkB p65 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-1803, NB100-224, NB100-376, NB100-464, NB500-160, NB100-381, NB200-347, NB100-2241, NBP1-06527

Alternate Names for RelA/NFkB p65 Antibody - BSA Free

• MGC131774
• nf kb p65
• NFkB p65
• NF-kB p65
• NFKB3
• NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
• Nuclear factor NF-kappa-B p65 subunit
• Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
• p65
• p65RelA
• rela p65
• RelA
• v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RelA/NFkB p65 Antibody (NB100-97831)(1)

Publication using NB100-97831

• Pierce GL, Lesniewski LA, Lawson BR et al. Nuclear Factor-{kappa}B Activation Contributes to Vascular Endothelial Dysfunction via Oxidative Stress in Overweight/Obese Middle-Aged Older Humans. Circulation;119(9):1284-1292. 2009-01-01 [PMID: 19237660]

Bioinformatics

• Gene Symbol: RELA
• Entrez:
  • 5970(Human)
• Uniprot:
  • Q04206()