RelA/NFkB p65 Antibody

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Proximity Ligation Assay: RelA/NFkB p65 Antibody [NB100-97831] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human CHK1 (NB100-274) and rabbit anti-human RelA (NB100-97831). Image merged from DAPI (2ms) ...read more
Proximity Ligation Assay: RelA/NFkB p65 Antibody [NB100-97831] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human CHK1 (NB100-311) and rabbit anti-human RelA (NB100-97831). Image merged from DAPI ...read more
Proximity Ligation Assay: RelA/NFkB p65 Antibody [NB100-97831] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human FUS (NBP2-14860) and rabbit anti-human RelA (NB100-97831). Image merged from DAPI (2ms) ...read more
Proximity Ligation Assay: RelA/NFkB p65 Antibody [NB100-97831] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC1 (NB100-230) and rabbit anti-human RelA (NB100-97831). Image merged from DAPI (2ms) ...read more
Immunoprecipitation: RelA/NFkB p65 Antibody [NB100-97831] - Detection of Human RelA/p65 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP, PLA, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

       

Order Details

RelA/NFkB p65 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human reticuloendotheliosis viral oncogene homolog A using the numbering given in entry NP_068810.2 (GeneID 5970).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RELA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:1000-1:10000
Application Notes
It is not recommended for western analysis.
Positive Control
RelA/NFkB p65 Lysate (NBL1-15276)
Publications
Read Publication using NB100-97831.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-1803, NB100-224, NB100-376, NB100-464, NB500-160, NB100-381, NB200-347, NB100-2241, NBP1-06527

Alternate Names for RelA/NFkB p65 Antibody

  • MGC131774
  • nf kb p65
  • NFkB p65
  • NF-kB p65
  • NFKB3
  • NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
  • Nuclear factor NF-kappa-B p65 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
  • p65
  • p65RelA
  • rela p65
  • RelA
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RelA/NFkB p65 Antibody (NB100-97831)(1)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on RelA/NFkB p65.

The effect of antioxidants and the NFkB p65 pathway in inflammation
NFkB is a transcription factor that plays a role in the expression of genes involved in immune response, inflammation, metastasis, cell survival and more. RelA (p65) is one member of the NFkB mammalian family, alongside other subunits. NFkB subunit...  Read full blog post.

NFkB3-p65: Say that three times fast!
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus...  Read full blog post.

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Bioinformatics

Gene Symbol RELA
Entrez
Uniprot