RelA/NFkB p65 [p Ser529] Antibody

Product Discontinued

RelA/NFkB p65 [p Ser529] Antibody Summary

• Immunogen: Synthetic phosphopeptide derived from human NFkB-p65 around the phosphorylation site of Serine 529.
• Modification: p Ser529
• Specificity: Detects NFkB p65 only when phosphorylated at Ser529
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RELA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: Western blot analysis of NFkB p65 [p Ser529] Antibody in extracts from 'HeLa cell treated with Insulin 0.01U/ml 15' or COS7cell treated with Insulin 0.01U/ml 2' Immunohistochemistry analysis of NFkB p65 [p Ser529] Antibody in paraffin-embedded human tonsil tissue
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  RelA/NFkB p65 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RelA/NFkB p65 [p Ser529] Antibody

• MGC131774
• nf kb p65
• NFkB p65
• NF-kB p65
• NFKB3
• NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
• Nuclear factor NF-kappa-B p65 subunit
• Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
• p65
• p65RelA
• rela p65
• RelA
• v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFkappaB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp kappaB sequence in the immunoglobulin kappa light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFkappaB is activated and NFkappaB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins have been described, designated p105 and p100. The p105 precursor contains p50 at its amino-terminus and a C-terminal region that when expressed as a separate molecule, designated PDI, binds to p50 and regulates its activity.The NFkappaB transcription factor is a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit, also referred to as RelA, is functionally related to c-Rel p75 and RelB p68. NFkappaB p65 is phosphorylated at Serine 276 as a response to TNF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RELA
• Entrez:
  • 5970(Human)
  • 19697(Mouse)
  • 309165(Rat)
• Uniprot:
  • Q04206()