Recombinant Rat IL-17F Protein

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Binding Activity

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Recombinant Rat IL-17F Protein Summary

Details of Functionality
Measured by its ability to compete with immobilized rmIL-17F for binding with rmIL-17R in a functional ELISA.
Source
E. coli-derived rat IL-17F protein
Ala19-Ala153
Accession #
N-terminal Sequence
Ala19
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il17f
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat IL-17F Protein

  • Cytokine ML-1
  • IL17F
  • IL-17F
  • IL-17Finterleukin-17F
  • IL24
  • IL-24
  • interleukin 17F
  • Interleukin-24
  • ML-1
  • ML1interleukin-24

Background

Interleukin-17F (also ML-1) is a 19 kDa member of the IL-17 family of cytokines. Members of this family are involved in tissue homeostasis and demonstrate a structural motif termed a cysteine knot that characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1, 2, 3, 4). Based on mouse, mature rat IL-17F is 133 amino acids (aa) in length (5, 6). Rat IL-17F is a presumably secreted, 38 kDa glycosylated disulfide-linked homodimer. It is also secreted as a 35 kDa disulfide-linked heterodimer with IL-17/17A (7, 8). The heterodimeric form represents about 30% of secreted IL-17F. Initially, IL-17F was also reported as IL-24. Since that time, the IL-24 designation has been reassigned to MDA-7, a member of the IL-10 family of molecules (note: IL-17E is synonymous with IL-25). Mature rat IL-17F shares 59% and 90% aa identical with mature human and mouse IL-17F, respectively; it also shows 55% aa identity to rat IL-17. Interspecies studies suggest rat IL-17F is produced by activated TH17-type CD4+ T cells, mast cells, basophils and monocytes (1, 3, 9), and is inducible through the interaction of TGF-beta, IL-6 and IL-23 (9, 10, 11). Targets for IL-17F include respiratory epithelium, fibroblasts, macrophages and endothelial cells which produce proinflammatory cytokines such as GM-CSF, IL-6, IFN-gamma, IP-10, MIP-1 alpha and MCP-1 (2, 6, 12). This activity is found for both homodimeric and heterodimeric forms of IL-17F (7).

  1. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  2. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
  3. Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:801.
  4. Gaffen, S.H. et al. (2006) Vitam. Horm. 74:255.
  5. Genbank Accession # : NP_001015011.
  6. Oda, N. et al. (2005) Am. J. Respir. Cell Mol. Biol. 171:12.
  7. Chang, S.H. and C. Gong (2007) Cell Res. 17:435.
  8. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  9. Kawaguchi, M. et al. (2001) J. Immunol. 167:4430.
  10. Langrish, C.L. et al. (2005) J. Exp. Med. 201:233.
  11. Yang, X.O. et al. (2007) J. Biol. Chem. 282:9358.
  12. Mcallister, F. et al. (2005) J. Immunol. 175:404.

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Bioinformatics

Gene Symbol Il17f
Entrez
Uniprot