Recombinant Rat E-Selectin/CD62E Fc Chimera Protein, CF

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat E-Selectin/CD62E Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10⁴ cells/well are added to rat E-Selectin/Fc Chimera coated plates (2 µg/mL with 100 µL/well), approximately 85-100% will adhere for 1 hour incubation at 37 °C.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived rat E-Selectin/CD62E protein
  

  Rat E-Selectin (Trp22 - Pro494) Accession # P98105	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: P98105
• N-terminal Sequence: Trp22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Sele
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 78 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 110-120 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat E-Selectin/CD62E Fc Chimera Protein, CF

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha , and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and rat proteins share approximately 67% amino acid identity. The mouse and rat proteins share approximately 78% amino acid identity.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

Publications for E-Selectin/CD62E (977-ES)(1)

Publication using 977-ES

• Ni</LastName><ForeName>Zh Z</Initial, Ni Z, Walcheck B Varied levels of reactivity by different E-selectin/Fc constructs with cutaneous lymphocyte-associated antigen (CLA)(+) CD4(+) T cells. Immunol. Lett., 2007-01-03;108(2):179-82. 2007-01-03 [PMID: 17222460] (Bioassay, Human)

Bioinformatics

• Gene Symbol: Sele
• Uniprot:
  • P98105()