Recombinant Rat CTGF Protein, CF

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Immobilized Recombinant Rat CTGF (Catalog # 9237-CT) supports the adhesion of Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 1-5 μg/mL

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Rat CTGF Protein, CF Summary

Details of Functionality
Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Ball, D.K. et al. (2003) Reproduction 125:271. The ED50 for this effect is 1-5 μg/mL
Source
Human embryonic kidney cell, HEK293-derived rat CTGF/CCN2 protein
Gln25-Ala347
Accession #
N-terminal Sequence
No results obtained. Gln25 inferred from enzymatic pyroglutamate treatment revealing Asp26
Protein/Peptide Type
Recombinant Proteins
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
35-41 kDa, reducing conditions
Publications
Read Publication using
9237-CT in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat CTGF Protein, CF

  • CCN2
  • CCN2IGFBP-8
  • connective tissue growth factor
  • CTGF
  • CTGRP
  • Fisp12
  • HCS24
  • Hypertrophic chondrocyte-specific protein 24
  • IBP-8
  • IGF-binding protein 8
  • IGFBP-8
  • IGFBP8CCN family member 2
  • Insulin-like growth factor-binding protein 8
  • MGC102839
  • NOV2

Background

Connective Tissue Growth Factor (CTGF), also known as CCN2, is a 36-38 kDa member of the CCN (CYR61/CTGF/NOV) family of secreted matricellular proteins (1). Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain (2). Rat CTGF shares 94% and 99% amino acid (aa) sequence identity with human and mouse CTGF, respectively. CTGF promotes cell adhesion through interactions with a range of cell surface molecules including heparan sulfate proteoglycans (HSPG), LRPAP, and Integrins alpha M, alpha V beta 3, alpha 6 beta 1, alpha IIb beta 3 (3-7). It also binds to and regulates signaling through the Wnt receptors LRP6 and Frizzled-8 and the NGF receptors TrkA and NGF R (8, 9). In addition, CTGF binds directly to BMP-4, TGF-beta 1, TGF-beta 2, and VEGF 165 (10, 11). It blocks BMP-4 and VEGF induced responses but enhances TGF-beta induced responses (10-13). Within VEGF complexes, CTGF can be degraded by a variety of proteases, resulting in restoration of angiogenic activity (11). CTGF promotes fibroblast differentiation from mesenchymal stem cells and their production of type I Collagen and Tenascin C (5, 14). It promotes chondrocyte proliferation and cartilage matrix synthesis (15-17). CTGF is expressed in vascular mural and endothelial cells (EC) during development and promotes pericyte-EC association and angiogenesis (2, 17, 18, 19). It is expressed in the cerebral cortex and olfactory bulb and plays an important role in nervous system development (8, 12).
  1. Hall-Glenn, F. and K.M. Lyons (2011) Cell. Mol. Life Sci. 68:3209.
  2. Xu, J. et al. (2000) J. Cell. Biochem. 77:103.
  3. Gao, R. and D.R. Brigstock (2004) J. Biol. Chem. 279:8848.
  4. Schober, J.M. et al. (2002) Blood 99:4457.
  5. Heng, E.C.K. et al. (2006) J. Cell Biochem. 98:409.
  6. Jedsadayanmata, A. et al. (1999) J. Biol. Chem. 274:24321.
  7. Gao, R. et al. (2003) Hepatol. Res. 27:214.
  8. Mercurio, S. et al. (2004) Development 131:2137.
  9. Wahab, N.A. et al. (2005) J. Am. Soc. Nephrol. 16:340.
  10. Abreu, J.G. et al. (2002) Nat. Cell Biol. 4:599.
  11. Hashimoto, G. et al. (2002) J. Biol. Chem. 277:36288.
  12. Khodosevich, K. et al. (2013) Neuron 79:1136.
  13. Shi-Wen, X. et al. (2006) J. Biol. Chem. 281:10715.
  14. Lee, C.H. et al. (2010) J. Clin. Invest. 120:3340.
  15. Canalis, E. et al. (2010) Endocrinology 151:3490.
  16. Nakanishi, T. et al. (2000) Endocrinology 141:264.
  17. Ivkovic, S. et al. (2003) Development 130:2779.
  18. Hall-Glenn, F. et al. (2012) PLoS One 7:e30562.
  19. Shimo, T. et al. (1999) J. Biochem. 126:137.

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Publications for CTGF/CCN2 (9237-CT)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Bioassay.


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FAQs for CTGF/CCN2 (9237-CT). (Showing 1 - 1 of 1 FAQ).

  1. We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
    • Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.
Recombinant Monoclonal Antibodies

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