Recombinant Rat CNTFR alpha Protein

• Reactivity: Rt
• Applications: Bioactivity

Recombinant Rat CNTFR alpha Protein Summary

• Details of Functionality: Measured by its ability to enhance CNTF-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.05-0.15 µg/mL in the presence of 1 ng/mL of recombinant rat CNTF.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat CNTF R alpha protein
  Ala19-Pro346 & Gln23-Pro346
• Accession #: Q08406
• N-terminal Sequence:

  Ala19 & Gln23

• Protein/Peptide Type: Recombinant Proteins
• Gene: Cntfr
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 39.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 52 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat CNTFR alpha Protein

• Ciliary neurotrophic factor receptor subunit alpha;Cntfr
• CNTF R alpha
• CNTFR alpha
• CNTFR
• CNTFRa

Background

The high-affinity CNTF receptor complex, which mediates the biological action of CNTF, contains three proteins: the ligand-binding a subunit (CNTF R alpha ) and the two signal-transducing proteins LIF R beta and gp130. Whereas LIF R beta and gp130 are widely expressed in many cell types, the expression of CNTF R alpha is restricted to the central and peripheral nervous systems. cDNAs encoding CNTF R alpha have been isolated from both human and rat and were shown to share 94% amino acid (aa) sequence identity. Rat CNTF R alpha cDNA encodes a 372 amino acid residue precursor protein that apparently has a 22 aa residue signal peptide and five potential glycoslyation sites. CNTF R alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage. Similar to other GPI-linked proteins, soluble CNTF receptor alpha (CNTF sR alpha ) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid. CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTF R alpha .

1. Yancopoulos, G.D. in Guidebook to Cytokines and Their Receptors,
Nicola, N.A. editor, Oxford University Press, New York, pp137.

Publications for CNTFR alpha (558-CR)(2)

Publications using 558-CR

• CNTF Attenuates Vasoproliferative Changes Through Upregulation of SOCS3 in a Mouse-Model of Oxygen-Induced Retinopathy Invest Ophthalmol Vis Sci, 2016-08-01;57(10):4017-26. 2016-08-01 [PMID: 27494343] (Bioassay, Human)
• Mizisin AP, Vu Y, Shuff M, Calcutt NA Ciliary neurotrophic factor improves nerve conduction and ameliorates regeneration deficits in diabetic rats. Diabetes, 2004-07-01;53(7):1807-12. 2004-07-01 [PMID: 15220205] (In Vivo, Rat)

Bioinformatics

• Gene Symbol: Cntfr
• Uniprot:
  • Q08406()