Recombinant Porcine IL-1 beta/IL-1F2 Protein

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Summary
Reactivity PoSpecies Glossary
Applications Bioactivity

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Recombinant Porcine IL-1 beta/IL-1F2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 0.75-4.5 ng/mL.
Source
E. coli-derived porcine IL-1 beta/IL-1F2 protein
Ala115-Pro267
Accession #
N-terminal Sequence
Ala115
Protein/Peptide Type
Recombinant Proteins
Gene
IL1B1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
681-PI in the following applications:

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Porcine IL-1 beta/IL-1F2 Protein

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63%-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL-1 beta.

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Huether, M. et al. (1993) Gene 129:285.
  6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

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Publications for IL-1 beta/IL-1F2 (681-PI)(7)

We have publications tested in 3 confirmed species: Bovine, N/A, Porcine.

We have publications tested in 4 applications: Bioassay, ELISA Standard, ELISA development, In Vivo.


Filter By Application
Bioassay
(3)
ELISA Standard
(1)
ELISA development
(2)
In Vivo
(1)
All Applications
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Bovine
(1)
N/A
(1)
Porcine
(5)
All Species
Showing Publications 1 - 7 of 7.
Publications using 681-PI Applications Species
Park H, Liu G, Liu Q, Zhou Y Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production. Viruses, 0;10(8):. 0 [PMID: 30096906] (ELISA development, Porcine) ELISA development Porcine
Park H, Liu G, Thulasi Raman S, Landreth S, Liu Q, Zhou Y NS1 Protein of 2009 Pandemic Influenza A Virus Inhibits Porcine NLRP3 Inflammasome-Mediated Interleukin-1 Beta Production by Suppressing ASC Ubiquitination. J Virol, 0;92(8):. 0 [PMID: 29386291] (ELISA development, Porcine) ELISA development Porcine
Comblain F, Sanchez C, Lesponne I, Balligand M, Serisier S, Henrotin Y Curcuminoids extract, hydrolyzed collagen and green tea extract synergically inhibit inflammatory and catabolic mediator&#039;s synthesis by normal bovine and osteoarthritic human chondrocytes in monolayer. PLoS ONE, 2015;10(3):e0121654. 2015 [PMID: 25799427] (Bioassay, Bovine) Bioassay Bovine
Delputte PL, Van Breedam W, Barbe F, Van Reeth K, Nauwynck HJ IFN-alpha treatment enhances porcine Arterivirus infection of monocytes via upregulation of the porcine Arterivirus receptor sialoadhesin. J. Interferon Cytokine Res., 2007;27(9):757-66. 2007 [PMID: 17892397] (Bioassay, Porcine) Bioassay Porcine
Kelley MJ, Rose AY, Song K, Chen Y, Bradley JM, Rookhuizen D, Acott TS Synergism of TNF and IL-1 in the induction of matrix metalloproteinase-3 in trabecular meshwork. Invest. Ophthalmol. Vis. Sci., 2007;48(6):2634-43. 2007 [PMID: 17525194] (Bioassay, Porcine) Bioassay Porcine
Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology. Cytometry A, 2006;69(5):391-5. 2006 [PMID: 16604540] (ELISA Standard, N/A) ELISA Standard N/A
Wheaton AJ, Dodge GR, Borthakur A, Kneeland JB, Schumacher HR, Reddy R Detection of changes in articular cartilage proteoglycan by T(1rho) magnetic resonance imaging. J. Orthop. Res., 2005;23(1):102-8. 2005 [PMID: 15607881] (In Vivo, Porcine) In Vivo Porcine

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Blogs on IL-1 beta/IL-1F2.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

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Bioinformatics

Gene Symbol IL1B1
Uniprot