Recombinant Mouse WISP-1/CCN4 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse WISP-1/CCN4 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. The ED₅₀ for this effect is 1.5 -9 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse WISP-1/CCN4 protein
  Thr23-Asn367, with a C-terminal 10-His tag
• Accession #: O54775
• N-terminal Sequence: Thr23
• Protein/Peptide Type: Recombinant Proteins
• Gene: Wisp1
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 39.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse WISP-1/CCN4 Protein, CF

• CCN family member 4
• CCN4
• CCN4WISP1tc
• WISP1
• WISP-1
• WISP1c
• WISP1i
• WNT1 induced secreted protein 1
• WNT1 inducible signaling pathway protein 1
• Wnt-1 inducible signaling pathway protein 1
• wnt-1 signaling pathway protein 1
• Wnt-1-induced secreted protein
• WNT1-inducible-signaling pathway protein 1

Background

Mouse WISP-1 (Wnt-induced secreted protein-1; also named CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1-6). It is synthesized as a 367 amino acid (aa) precursor that contains a series of structural homology modules. Following a 22 aa signal sequence, there is a 68 aa IGFBP-like domain (aa 53-120), a 66 aa von Willebrand factor type C (VWC) module (aa 121-186), a 41 aa TSP type I domain (aa 220-260) and a 75 aa, C-terminal cysteine knot motif (aa 273-347) (1, 6). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). Multiple splice forms are reported for human WISP-1, but none for rodent (7-9). Mature mouse WISP-1 is 98%, 84% and 85% aa identical to rat, canine and human WISP-1, respectively. WISP-1 is expressed by fibroblasts and osteoblasts, and may contribute to fracture healing by promoting bone cell formation (1, 10, 11).

1. Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
2. Tanada, S. et al. (2001) Oncogene 20:5525.
3. Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
4. Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
5. Brigstock, D.R. (2003) J. Endocrinol. 178:169.
6. Hashimoto, Y. et al. (1998) J. Exp. Med. 187:289.
7. SwissProt Accession # O54775.
8. SwissProt Accession # Q99PP0.
9. SwissProt Accession # O95388
10. French, D.M. et al. (2004) Am. J. Pathol. 165:855.
11. Parisi, M.S. et al. (2006) Bone 38:671.

Publications for WISP-1/CCN4 (1680-WS)(5)

Publications using 1680-WS

• DJ Klinke Ii, A Fernandez, W Deng, A Razazan, H Latifizade, AC Pirkey Data-driven learning how oncogenic gene expression locally alters heterocellular networks Nature Communications, 2022-04-13;13(1):1986. 2022-04-13 [PMID: 35418177] (Bioassay, Mouse)
• R Fernandez-, A García-Ala, Y Esteban, J Mir-Coll, B Serra-Nava, M Fontcubert, C Broca, M Armanet, A Wojtuscisz, V Kram, MF Young, J Vidal, R Gomis, R Gasa Wisp1 is a circulating factor that stimulates proliferation of adult mouse and human beta cells Nat Commun, 2020-11-25;11(1):5982. 2020-11-25 [PMID: 33239617] (Bioassay, In vivo assay, Mouse)
• W Deng, A Fernandez, SL McLaughlin, DJ Klinke WNT1-inducible signaling pathway protein 1 (WISP1/CCN4) stimulates melanoma invasion and metastasis by promoting the epithelial - mesenchymal transition J. Biol. Chem., 2019-02-05;0(0):. 2019-02-05 [PMID: 30723155] (Cell Culture, Human)
• L Lukjanenko, S Karaz, P Stuelsatz, U Gurriaran-, J Michaud, G Dammone, F Sizzano, O Mashinchia, S Ancel, E Migliavacc, S Liot, G Jacot, S Metairon, F Raymond, P Descombes, A Palini, B Chazaud, MA Rudnicki, CF Bentzinger, JN Feige Aging Disrupts Muscle Stem Cell Function by Impairing Matricellular WISP1 Secretion from Fibro-Adipogenic Progenitors Cell Stem Cell, 2019-01-24;0(0):. 2019-01-24 [PMID: 30686765] (In Vivo, Mouse)
• Quan Li MECHANICAL VENTILATION AUGMENTS POLY(I:C)INDUCED LUNG INJURY VIA A WISP1-INTEGRIN ?3 DEPENDENT PATHWAY IN MICE Mol. Med., 2016-01-06;0(0):. 2016-01-06 [PMID: 26772774] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Wisp1
• Uniprot:
  • O54775()