Recombinant Mouse ULBP-1/MULT-1 Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse ULBP-1/MULT-1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse NKG2D Fc Chimera at 2 µg/mL (100 µL/well) can bind Recombinant Mouse ULBP‑1/MULT-1 with a linear range of 1-50 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse ULBP-1 protein
  Pro26-Thr211, with a C-terminal 6-His tag
• Accession #: NP_084251
• N-terminal Sequence: Pro26
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ulbp1
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 22 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 28-43 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ULBP-1/MULT-1 Protein, CF

• ALCAN-beta
• MULT-1
• N2DL1
• N2DL-1
• NKG2D ligand 1
• NKG2DL
• NKG2DL1
• RAET1I
• RAET1Ialcan-beta
• Retinoic acid early transcript 1I
• UL16 binding protein 1
• UL16-binding protein 1
• ULBP1
• ULBP-1

Background

MULT-1 (mouse UL16-binding protein-like Transcript 1) is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands (1-4). It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail (2). The extracellular region contains an alpha-1 and alpha-2 like domain with two intrachain disulfide bonds. MULT-1 is distantly related to other human and mouse NKG2D ligands, and more distantly related to the MHC class I proteins (3). Unlike most NKG2D ligands, transcripts for MULT-1 have been detected in a wide variety of mouse tissues and tumor cells lines (3). The receptor for MULT-1 is NKG2D, a 35 kDa C-type lectin that is found on mouse NK cells, activated CD8⁺ T cells, epidermal gamma δ T cells, and activated macrophages (1, 5, 6, 7). Recombinant MULT-1 protein binds to NKG2D with high affinity (K_d = 6 nM) (2). Although an activating receptor, general cellular responses to NKG2D ligation depend upon the isoform of NKG2D and the cell type (5). Little is known about the specific MULT-1-NKG2D association. Exposure to immobilized MULT-1 or MULT-1-transfected cells elicits IFN-gamma production by NK cells (3). Ectopic expression of MULT-1 on the RMA mouse tumor cell line leads to tumor rejection in syngeneic mice (3).

1. Raulet, D.H. (2003) Nat. Rev. Immunol. 3:781.
2. Carayannopoulos, L. et al. (2002) J. Immunol. 169:4079.
3. Diefenbach, A. et al. (2003) Eur. J. Immunol. 33:381.
4. Krmpotic, A. et al. (2005) J. Exp. Med. 201:211.
5. Diefenbach, A. et al. (2002) Nat. Immunol. 3:1142.
6. Ho, E.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6320.
7. Carayannopoulos, L.N. et al. (2002) Eur. J. Immunol. 32:597.

Bioinformatics

• Gene Symbol: Ulbp1
• Uniprot:
  • NP_084251()