Recombinant Mouse TSLP Protein

Images

 
There are currently no images for TSLP (555-TS).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Mouse TSLP Protein Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse IL-7 R alpha. The ED50 for this effect is 0.5-2.5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse TSLP protein
Tyr20-Glu140, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Tyr20
Protein/Peptide Type
Recombinant Proteins
Gene
Tslp
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
22-29 kDa, reducing conditions
Publications
Read Publications using
555-TS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TSLP Protein

  • thymic stromal lymphopoietin
  • TSLP

Background

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Mouse TSLP cDNA encodes a 140 amino acid (aa) residue precursor protein with a 19 aa signal sequence. Within the mature region, there are three potential N-glycosylation sites and 7 cysteine residues. Mouse TSLP shares approximately 43% aa sequence identity with human TSLP. The gene for mouse TSLP has been localized to chromosome 18. By Northern and RT-PCR analysis, mouse TSLP expression has been detected in spleen, thymus, kidney, lung and bone marrow. TSLP is proposed to signal through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a new member of the hemopoietin receptor family most closely related to R gamma c.

  1. Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
  2. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  3. Pandey, A. et al. (2000) Nature Immunol. 1:59.
  4. Reche, P.A. et al. (2001) J. Immunol. 167:336.

Customers Who Viewed This Item Also Viewed...

AF981
Species: Hu
Applications: WB, IHC, ICC, Neut
6507-IL/CF
Species: Hu
NBP1-19371
Species: Hu, Mu, Rt, Po, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-72042
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
MAB10541
Species: Hu
Applications: Flow, CyTOF-ready, ICC, Neut
NBP2-24875
Species: Hu, Mu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, BA, B/N, Flow-IC
NB600-922
Species: Ch, V-Vi
Applications: WB, ELISA, IHC, IHC-P, IP, Single-Cell Western
NB110-97871
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready

Publications for TSLP (555-TS)(28)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Bioassay, Cell Culture, Enzyme Assay, In Vivo.


Filter By Application
Bioassay
(16)
Cell Culture
(3)
Enzyme Assay
(1)
In Vivo
(8)
All Applications
Filter By Species
Human
(1)
Mouse
(27)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using 555-TS Applications Species
K Arae, M Ikutani, K Horiguchi, S Yamaguchi, Y Okada, H Sugiyama, K Orimo, H Morita, H Suto, K Okumura, H Taguchi, K Matsumoto, H Saito, K Sudo, S Nakae Interleukin-33 and thymic stromal lymphopoietin, but not interleukin-25, are crucial for development of airway eosinophilia induced by chitin Scientific Reports, 2021;11(1):5913. 2021 [PMID: 33723298] (Bioassay, Mouse) Bioassay Mouse
F Yao, Q Yuan, X Song, L Zhou, G Liang, G Jiang, L Zhang Yupingfeng Granule Improves Th2-Biased Immune State in Microenvironment of Hepatocellular Carcinoma through TSLP-DC-OX40L Pathway Evid Based Complement Alternat Med, 2020;2020(0):1263053. 2020 [PMID: 32351590] (Cell Culture, Mouse) Cell Culture Mouse
SK Mishra, JJ Wheeler, S Pitake, H Ding, C Jiang, T Fukuyama, JS Paps, P Ralph, J Coyne, M Parkington, J DeBrecht, LC Ehrhardt-H, GP Cruse, W Bäumer, RR Ji, MC Ko, T Olivry Periostin Activation of Integrin Receptors on Sensory Neurons Induces Allergic Itch Cell Rep, 2020;31(1):107472. 2020 [PMID: 32268102] (Bioassay, Mouse) Bioassay Mouse
S Li, Z Yi, M Deng, MJ Scott, C Yang, W Li, Z Lei, NM Santerre, P Loughran, TR Billiar TSLP protects against liver I/R injury via activation of the PI3K/Akt pathway JCI Insight, 2019;4(22):. 2019 [PMID: 31723054] (Bioassay, Mouse) Bioassay Mouse
E Ragonnaud, K Moritoh, M Bodogai, F Gusev, S Garaud, C Chen, X Wang, T Baljinnyam, KG Becker, RW Maul, K Willard-Ga, E Rogaev, A Biragyn Tumor-derived Thymic stromal lymphopoietin expands bone marrow B-cell precursors in circulation to support metastasis Cancer Res., 2019;0(0):. 2019 [PMID: 31575547] (Bioassay, Mouse) Bioassay Mouse
NR Han, PD Moon, HM Kim, HJ Jeong TSLP Exacerbates Septic Inflammation via Murine Double Minute 2 (MDM2) Signaling Pathway J Clin Med, 2019;8(9):. 2019 [PMID: 31480519] (In Vivo, Mouse) In Vivo Mouse
N Inagaki-Ka, T Ito, M Inaba, Y Azuma, A Tanaka, PTX Vien, K Kibata, A Satake, S Nomura Statins can suppress dendritic cell-mediated Th2 responses through the repression of OX40-ligand and CCL17 expression Eur. J. Immunol., 2019;0(0):. 2019 [PMID: 31269241] (Cell Culture, Mouse) Cell Culture Mouse
JH Bernink, Y Ohne, MBM Teunissen, J Wang, J Wu, L Krabbendam, C Guntermann, R Volckmann, J Koster, S van Tol, I Ramirez, Y Shrestha, MA de Rie, H Spits, X Romero Ros, AA Humbles c-Kit-positive ILC2s exhibit an ILC3-like signature that may contribute to IL-17-mediated pathologies Nat. Immunol., 2019;0(0):. 2019 [PMID: 31263279] (Cell Culture, Human) Cell Culture Human
M Han, C Rajput, JY Hong, J Lei, JL Hinde, Q Wu, JK Bentley, MB Hershenson The Innate Cytokines IL-25, IL-33, and TSLP Cooperate in the Induction of Type 2 Innate Lymphoid Cell Expansion and Mucous Metaplasia in Rhinovirus-Infected Immature Mice J. Immunol., 2017;0(0):. 2017 [PMID: 28701507] (Bioassay, Mouse) Bioassay Mouse
L Kummola, Z Ortutay, X Chen, S Caucheteux, S Hämäläinen, S Aittomäki, R Yagi, J Zhu, M Pesu, WE Paul, IS Junttila IL-7R? Expression Regulates Murine Dendritic Cell Sensitivity to Thymic Stromal Lymphopoietin J. Immunol., 2017;0(0):. 2017 [PMID: 28404633] (Bioassay, Mouse) Bioassay Mouse
Show All 28 Publications.

Reviews for TSLP (555-TS) (0)

There are no reviews for TSLP (555-TS). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for TSLP (555-TS) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

TSLP Antibody
NB110-55234

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse TSLP Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol Tslp
Uniprot