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Recombinant Mouse TRANCE/RANK L/TNFSF11 Protein, CF


Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR/CF) induces osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cell line. The ED50 for this effect is 5-15 ng/mL in the presence of 2.5 μg/mL more
1 μg/lane of Recombinant Mouse TRANCE/TNFSF11/RANK L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 36 kDa.

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Recombinant Mouse TRANCE/RANK L/TNFSF11 Protein, CF Summary

Details of Functionality
Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 5‑15 ng/mL in the presence of 2.5 µg/mL of a
cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
An E. coli-expressed Recombinant Mouse (rm) TRANCE (aa 158-317) (Catalog # 462-TEC) is also available. The ED50 for the E. coli-expressed rmTRANCE is 0.5‑2 ng/mL.
Mouse myeloma cell line, NS0-derived mouse TRANCE/TNFSF11/RANK L protein
Arg72-Asp316, with an N-terminal 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
36 kDa, reducing conditions
Read Publications using
462-TR/CF in the following applications:

Packaging, Storage & Formulations

  • 3 months from date of receipt, 2 to 8 °C as supplied.
Supplied as a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TRANCE/RANK L/TNFSF11 Protein, CF

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11


TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokines, osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. In the TNF superfamily nomenclature, TRANCE is referred to as TNFSF11. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The murine TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

  1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
  2. Anderson, D.M. et al. (1997) Nature 390:175.
  3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
  4. Kong, Y-Y. et al. (1999) Nature 397:315.

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Publications for TRANCE/TNFSF11/RANK L (462-TR/CF)(86)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), In Vivo.

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Showing Publications 1 - 10 of 86. Show All 86 Publications.
Publications using 462-TR/CF Applications Species
X Hu, SW Wong, K Liang, TH Wu, S Wang, L Wang, J Liu, M Yamauchi, BL Foster, JP Ting, B Zhao, HC Tseng, CC Ko Optineurin regulates NRF2-mediated antioxidant response in a mouse model of Paget&#039;s disease of bone Science Advances, 2023-01-27;9(4):eade6998. 2023-01-27 [PMID: 36706179] (Bioassay, Mouse) Bioassay Mouse
CY Lin, X Song, Y Ke, A Raha, Y Wu, M Wasi, L Wang, F Geng, L You Yoda1 Enhanced Low-Magnitude High-Frequency Vibration on Osteocytes in Regulation of MDA-MB-231 Breast Cancer Cell Migration Cancers, 2022-07-13;14(14):. 2022-07-13 [PMID: 35884459] (Bioassay, Mouse) Bioassay Mouse
G Li, OJ Sul, R Yu, HS Choi 7-Ketocholesterol-Induced Micro-RNA-107-5p Increases Number and Activity of Osteoclasts by Targeting MKP1 International Journal of Molecular Sciences, 2022-03-28;23(7):. 2022-03-28 [PMID: 35409056] (Cell Culture, Mouse) Cell Culture Mouse
Q Xu, P Zhan, X Li, F Mo, H Xu, Y Liu, Q Lai, B Zhang, M Dai, X Liu Bisphosphonate-enoxacin inhibit osteoclast formation and function by abrogating RANKL-induced JNK signalling pathways during osteoporosis treatment Journal of Cellular and Molecular Medicine, 2021-10-15;0(0):. 2021-10-15 [PMID: 34651433] (Cell Culture, Mouse) Cell Culture Mouse
J Yu, S Choi, A Park, J Do, D Nam, Y Kim, J Noh, KY Lee, CH Maeng, KS Park Bone Marrow Homeostasis Is Impaired via JAK/STAT and Glucocorticoid Signaling in Cancer Cachexia Model Cancers, 2021-03-02;13(5):. 2021-03-02 [PMID: 33801569] (Cell Culture, Mouse) Cell Culture Mouse
Z Liu, Y Li, F Guo, C Zhang, G Song, J Yang, D Chen Tetrandrine Inhibits Titanium Particle-Induced Inflammatory Osteolysis through the Nuclear Factor-&amp;kappaB Pathway Mediators of Inflammation, 2020-11-27;2020(0):1926947. 2020-11-27 [PMID: 33312069] (Cell Culture, Mouse) Cell Culture Mouse
S Rosendahl, R Sulniute, M Eklund, C Koskinen H, MJO Johansson, E Kindstedt, S Lindquist, P Lundberg CCR3 deficiency is associated with increased osteoclast activity and reduced cortical bone volume in adult male mice The Journal of Biological Chemistry, 2020-12-17;0(0):. 2020-12-17 [PMID: 33303631] (Cell Culture, Mouse) Cell Culture Mouse
L Xiao, M Zhong, Y Huang, J Zhu, W Tang, D Li, J Shi, A Lu, H Yang, D Geng, H Li, Z Wang Puerarin alleviates osteoporosis in the ovariectomy-induced mice by suppressing osteoclastogenesis via inhibition of TRAF6/ROS-dependent MAPK/NF-&amp;kappaB signaling pathways Aging (Albany NY), 2020-11-07;12(21):21706-21729. 2020-11-07 [PMID: 33176281] (Cell Culture, Mouse) Cell Culture Mouse
AL Rocha, RL Bighetti-T, LF Duffles, JAA de Arruda, TM Taira, BRD Assis, S Macari, IMA Diniz, MM Beloti, AL Rosa, SY Fukada, GAC Goulart, DD Ribeiro, LG Abreu, TA Silva Inhibitory effects of dabigatran etexilate, a direct thrombin inhibitor, on osteoclasts and osteoblasts Thromb. Res., 2019-12-23;186(0):45-53. 2019-12-23 [PMID: 31883999] (Bioassay, Mouse) Bioassay Mouse
Y Zhang, Y Cui, L Wang, J Han Autophagy promotes osteoclast podosome disassembly and cell motility athrough the interaction of kindlin3 with LC3 Cell. Signal., 2019-12-17;67(0):109505. 2019-12-17 [PMID: 31857241] (Bioassay, Mouse) Bioassay Mouse
Show All 86 Publications.

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Gene Symbol Tnfsf11