Recombinant Mouse TRANCE/RANK L/TNFSF11 Protein, CF


1 μg/lane of Recombinant Mouse TRANCE/TNFSF11/RANK L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 36 kDa.
Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR/CF) induces osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cell line. The ED50 for this effect is 5-15 ng/mL in the presence of 2.5 μg/mL more

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Recombinant Mouse TRANCE/RANK L/TNFSF11 Protein, CF Summary

Details of Functionality
Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 5‑15 ng/mL in the presence of 2.5 µg/mL of a
cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
An E. coli-expressed Recombinant Mouse (rm) TRANCE (aa 158-317) (Catalog # 462-TEC) is also available. The ED50 for the E. coli-expressed rmTRANCE is 0.5‑2 ng/mL.
Mouse myeloma cell line, NS0-derived mouse TRANCE/TNFSF11/RANK L protein
Arg72-Asp316, with an N-terminal 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
36 kDa, reducing conditions
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462-TR/CF in the following applications:

Packaging, Storage & Formulations

  • 3 months from date of receipt, 2 to 8 °C as supplied.
Supplied as a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TRANCE/RANK L/TNFSF11 Protein, CF

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11


TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokines, osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. In the TNF superfamily nomenclature, TRANCE is referred to as TNFSF11. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The murine TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

  1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
  2. Anderson, D.M. et al. (1997) Nature 390:175.
  3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
  4. Kong, Y-Y. et al. (1999) Nature 397:315.

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Publications for TRANCE/TNFSF11/RANK L (462-TR/CF)(57)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Mousse, N/A.

We have publications tested in 3 applications: Bioassay, ELISA Standard, In Vivo.

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Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using 462-TR/CF Applications Species
MGA Broeren, I Di Ceglie, MB Bennink, PLEM van Lent, WB van den Be, MI Koenders, EN Blaney Dav, PM van der Kr, FAJ van de Loo Treatment of collagenase-induced osteoarthritis with a viral vector encoding TSG-6 results in ectopic bone formation PeerJ, 2018;6(0):e4771. 2018 [PMID: 29868252] (Bioassay, Mouse) Bioassay Mouse
I Di Ceglie, G Ascone, NAJ Cremers, AW Sloetjes, B Walgreen, T Vogl, J Roth, JS Verbeek, FAJ van de Loo, MI Koenders, PM van der Kr, AB Blom, MHJ van den Bo, PLEM van Lent Fc? receptor-mediated influx of S100A8/A9-producing neutrophils as inducer of bone erosion during antigen-induced arthritis Arthritis Res. Ther., 2018;20(1):80. 2018 [PMID: 29720243] (Bioassay, Mouse) Bioassay Mouse
H Quan, M Liang, N Li, C Dou, C Liu, Y Bai, W Luo, J Li, F Kang, Z Cao, X Yang, H Jiang, S Dong LncRNA-AK131850 Sponges MiR-93-5p in Newborn and Mature Osteoclasts to Enhance the Secretion of Vascular Endothelial Growth Factor a Promoting Vasculogenesis of Endothelial Progenitor Cells Cell. Physiol. Biochem., 2018;46(1):401-417. 2018 [PMID: 29590659] (Bioassay, Mouse) Bioassay Mouse
YH Hwang, KJ Kim, SJ Kim, SK Mun, SG Hong, YJ Son, ST Yee Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In Vivo Int J Mol Sci, 2018;19(3):. 2018 [PMID: 29562730] (Bioassay, Mouse) Bioassay Mouse
IR Orriss, S Lanham, D Savery, NDE Greene, P Stanier, R Oreffo, AJ Copp, GL Galea Spina bifida-predisposing heterozygous mutations in Planar Cell Polarity genes and Zic2 reduce bone mass in young mice Sci Rep, 2018;8(1):3325. 2018 [PMID: 29463853] (Bioassay, Mouse) Bioassay Mouse
Y Ma, H Yang, J Huang Icariin ameliorates dexamethasone?induced bone deterioration in an experimental mouse model via activation of microRNA?186 inhibition of cathepsin K Mol Med Rep, 2018;17(1):1633-1641. 2018 [PMID: 29257214] (Bioassay, Mousse) Bioassay Mousse
EM Grössinger, M Kang, L Bouchareyc, R Sarin, DR Haudenschi, LN Borodinsky, IE Adamopoulo Ca2+-Dependent Regulation of NFATc1 via KCa3.1 in Inflammatory Osteoclastogenesis J. Immunol., 2017;0(0):. 2017 [PMID: 29246953] (Bioassay, Mouse) Bioassay Mouse
MS Lombardi, C Gilliéron, M Berkelaar, C Gabay Salt-inducible kinases (SIK) inhibition reduces RANKL-induced osteoclastogenesis PLoS ONE, 2017;12(10):e0185426. 2017 [PMID: 28973003] (Bioassay, Mouse) Bioassay Mouse
NC Blixt, BK Faulkner, K Astleford, R Lelich, J Schering, E Spencer, R Gopalakris, ED Jensen, KC Mansky Class II and IV HDACs function as inhibitors of osteoclast differentiation PLoS ONE, 2017;12(9):e0185441. 2017 [PMID: 28953929] (Bioassay, Mouse) Bioassay Mouse
E Kindstedt, CK Holm, R Sulniute, I Martinez-C, R Lundmark, P Lundberg CCL11, a novel mediator of inflammatory bone resorption Sci Rep, 2017;7(1):5334. 2017 [PMID: 28706221] (Bioassay, Mouse) Bioassay Mouse
Show All 57 Publications.

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The diverse functions of RANKL/TRANCE/TNFSF11
RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t...  Read full blog post.

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Gene Symbol Tnfsf11