Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse TRAIL/TNFSF10 (Catalog # 1121-TL) that produces 50% of the optimal binding response is approximately 15-75 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse TNFRH3/TNFRSF26 protein
  

  Mouse TNFRH3 (Ser20-Lys162) Accession # P83626	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: P83626
• N-terminal Sequence: Ser20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Tnfrsf26
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 43.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera Protein, CF

• APO-3;DDR3;DR3;GEF720;LARD;PLEKHG5;TNFRSF12;TNFRSF25;TR3;TRAMP;Tumor necrosis factor receptor superfamily member 25;WSL-1;WSL-LR
• TNFRH3
• Tnfrsf24
• Tnfrsf26

Background

Mouse TNFRH3 (Tumor necrosis factor receptor homolog 3; designated TNFR superfamily member 26) is a member of the TNF receptor superfamily. Northern blot analysis indicates its expression in the mouse spleen and thymus, and by a variety of mouse lymphoid cell lines (1). Mature mouse TNFRH3 is a 21 kDa (predicted, unglycosylated), 185 amino acid (aa) type I transmembrane protein that contains a 145 aa extracellular domain (ECD) and a 19 aa cytoplasmic domain. The ECD contains two potential sites of N‑linked glycosylation and three TNFR-Cys repeats that characterize TNF receptors, while the cytoplasmic portion lacks a death domain (1). No ortholog to mouse TNFRH3 has been reported in human or rat (1). Two TNFRH3‑related mouse TNF receptor homologs, DcTRAIL R1 (TNFRSF23/TNFRH1, 35% aa sequence identity within the ECD) and DcTRAIL R2 (TNFRSF22/TNFRH2, 40% aa sequence identity within the ECD) are classed as TRAIL decoy receptors. One report indicates that TNFRH3 binding of transfected TRAIL/TNFSF10 is below limits of detection in a cell-based assay, and it is thus thought not to serve as a TRAIL decoy receptor (1). Similarly, binding was not detected to any of 38 human and mouse TNF superfamily members in a cell‑based assay (2). However, TNFRH3 does bind recombinant trimeric mouse TRAIL in R&D Systems’ direct ELISA assay.

1. Schneider, P. et al. (2003) J. Biol. Chem. 278:5444.
2. Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.

Bioinformatics

• Gene Symbol: Tnfrsf26
• Uniprot:
  • P83626()