Recombinant Mouse Syndecan-3 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Syndecan-3 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to inhibit the adhesion of A431 human epithelial carcinoma cells. When 5 x 10⁴ cells/well are added to mouse Syndecan-3 and human Fibronectin (0.5 μg/mL, Catalog # 1918-FN) coated plates, cell adhesion is inhibited in a dose dependent manner after 90 minutes at 37 °C. The ED₅₀ for this effect is 0.2-0.6 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Syndecan-3 protein
  Ala45-Glu384, with a C-terminal 6-His tag
• Accession #: Q64519
• N-terminal Sequence: Ala45
• Protein/Peptide Type: Recombinant Proteins
• Gene: Sdc3
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 35.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 90 ‑ 190 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Syndecan-3 Protein, CF

• KIAA0468
• N-Syndecan
• SDC3
• SDCN
• SYND3syndecan proteoglycan 3
• syndecan 3 (N-syndecan)
• syndecan 3
• syndecan neural type
• Syndecan3
• Syndecan-3

Background

Syndecan-3, also called N-syndecan, is one of four vertebrate syndecans that are principal carriers of heparan sulfate and chondroitin sulfate glycosaminoglycans (GAGs) (1 - 3). These type 1 transmembrane proteins show conserved cytoplasmic domains and divergent extracellular domains (1 - 3). Human Syndecan-3 is synthesized as a 442 amino acid (aa) core protein with a 44 aa signal sequence, a 343 aa extracellular domain (ECD), a 21 aa transmembrane (TM) region and a 34 aa cytoplasmic tail with a binding site for PDZ domains (1). The ECD of mouse Syndecan-3 shares 95%, 83%, 82% and 81% aa identity with of rat, human, canine and bovine Syndecan-3, respectively. Syndecan-3 contains four conserved closely-spaced GAG attachment sites near the N‑terminus and unique threonine-rich and mucin-like sequences near the membrane (4). Addition of glycan side chains results in an apparent size of 120 ‑ 150 kDa. Non-covalent homodimerization of Syndecan-3 or, potentially, heterodimerization with Syndecan-2 or -4, is dependent on the transmembrane domain (5). A cleavage site near the TM domain allows shedding of soluble ECD; the remainder of the molecule undergoes regulated intramembrane proteolysis (6). Syndecan-3 is expressed in the nervous system and at limb buds during development (1, 2). It is expressed on neuronal axons and Schwann cell perinodal processes, promoting nerve cell migration and synapse formation (7, 8). Roles in memory and body weight regulation have been described (2, 9, 10). Through localization of growth factors such as FGF2, HGF and TGF-beta , it regulates expression of molecules important for differentiation of muscle and bone, such as myogenin, BMP-2 and hedgehog family members (1, 2, 11 ‑ 13). In adults, it is up‑regulated during regeneration, such as following myocardial infarction (14).

1. Xian, X. et al. (2010) Cell Tissue Res. 339:31.
2. Reizes, O. et al. (2008) Int. J. Biochem. Cell Biol. 40:28.
3. Carey, D.J. et al. (1997) J. Biol. Chem. 272:2873.
4. ENTREZ protein Accession # Q64519.
5. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
6. Schultz, J.G. et al. (2003) J. Biol. Chem. 278:48651.
7. Hienola, A. et al. (2006) J. Cell Biol. 174:569.
8. Goutebroze, L. et al. (2003) BMC Neurosci. 4:29.
9. Kaksonen, M. et al. (2002) Mol. Cell. Neurosci. 21:158.
10. Strader. A.D. et al. (2004) J. Clin. Invest. 114:1354.
11. Cornelison, D.D.W. et al. (2004) Genes Dev. 18:2231.
12. Fisher, M.C. et al. (2006) Matrix Biol. 25:27.
13. Pacifici, M. et al. (2005) J. Bone Miner. Metab. 23:191.
14. Finsen, A.V. et al. (2004) Physiol. Genomics 16:301.

Publications for SDC3 (2734-SD)(1)

Publication using 2734-SD

• Nakajima, C;Sawada, M;Umeda, E;Takagi, Y;Nakashima, N;Kuboyama, K;Kaneko, N;Yamamoto, S;Nakamura, H;Shimada, N;Nakamura, K;Matsuno, K;Uesugi, S;Vep?ek, NA;Küllmer, F;Nasufovi?, V;Uchiyama, H;Nakada, M;Otsuka, Y;Ito, Y;Herranz-Pérez, V;García-Verdugo, JM;Ohno, N;Arndt, HD;Trauner, D;Tabata, Y;Igarashi, M;Sawamoto, K; Identification of the growth cone as a probe and driver of neuronal migration in the injured brain Nature communications 2024-03-09 [PMID: 38461182] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Sdc3
• Uniprot:
  • Q64519()