Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF

Recombinant Mouse Stanniocalcin 2/STC-2 (Catalog # 9406-SO) inhibits Recombinant Mouse Wnt-3a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 75‑450 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 75-450 ng/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
• Source: Mouse myeloma cell line, NS0-derived mouse Stanniocalcin 2/STC-2 protein
  Thr25-Arg296, with a C-terminal 6-His tag
• Accession #: O88452
• N-terminal Sequence: Thr25
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 33-44 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Stanniocalcin 2/STC-2 Protein, CF

• Stanniocalcin 2
• stanniocalcin-2
• STC2
• STC-2
• STC-2Stanniocalcin-related protein
• STC-related protein
• STCRP

Background

Mouse STC-2 (Stanniocalcin-2) is a secreted disulfide-linked homodimeric glycoprotein hormone that is related to the STC protein. It is first discovered from corpuscles of stannous, an endocrine gland unique to bony fish (1-3). The 272 amino acid (aa) mature mouse STC-2 has an 86% homology with the human form. STC‑2 has been described as having a role in human adult height and is involved in the GIANT consortium (4, 5). STC-2 is expressed in a wide variety of tissues. It regulates calcium and phosphate homeostasis (1-3), enhances mesenchymal stem cell survival (6), promotes cell proliferation in cervical cancer (7), suppresses breast cancer cell migration and invasion (8), promotes osteoblast differentiation (9), and inhibits longitudinal bone growth directly at the growth plate (10). It is also a biomarker for cervical and lung cancers (11, 12).Fform

1. Wagner, G.F. et al. (1995) Proc. Natl. Acad. Sci. 92:1871.
2. Zhang, J. et al. (1998) Protein Expression and Purification. 12:390.
3. Takei, Y. et al. (2012) Bone. 50:998.
4. Marouli, E. et al. (2017) Nature 542:186.
5. Holmes, D. (2017) Nature Reviews Endocrinology. 13:190.
6. Kim PH, et al. (2015) BMB Rep. 48:702.
7. Wang Y, et al. (2015) Biochem Biophys Res Commun. 466:362.
8. Hou J, et al. (2015) PLoS One. 10:e0122179.
9. Zhou J, et al. (2016) Mol Med Rep. 14:5653.
10. Wu S, et al. (2006) J Biol Chem. 281:5120.
11. Shen XJ, et al. (2014) Int J Clin Exp Pathol. 7: 8770.
12. Na SS, et al. (2015) Biochim Biophys Acta. 1854:668.

Bioinformatics

• Uniprot:
  • O88452()