Recombinant Mouse SorCS2 Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse SorCS2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rmSorCS2 at 1 µg/mL (100 µL/well) can bind rh beta -NGF with an apparent K_D <75 nM.
• Source: Mouse myeloma cell line, NS0-derived mouse SorCS2 protein
  Ala118-Gly1078, with a C-terminal 6-His tag
• Accession #: Q9EPR5
• N-terminal Sequence: Ala118
• Protein/Peptide Type: Recombinant Proteins
• Gene: Sorcs2
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 108.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 120-125 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SorCS2 Protein, CF

• KIAA1329VPS10 domain receptor protein
• SorCS2
• sortilin-related VPS10 domain containing receptor 2
• VPS10 domain-containing receptor SorCS2

Background

SorCS2 is a type I transmembrane glycoprotein receptor of the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). These sorting receptors include sortilin, SorLA, and three SorCS proteins. All SorCS proteins are predominantly expressed in the brain, especially during development, but vary in their specific locations (1 - 3). SorCS2 mRNA has also been found in the kidney, lung, testis and heart (1, 2). SorCS2 presumably mediates endocytosis, but its ligands are not well-defined. However, the other SorCS proteins have been shown to bind NGF and PDGF-BB (4 - 6). Mouse SorCS2 is synthesized as a 1159 amino acid (aa) preproform with a 49 aa signal sequence and a potential furin-type proteolytic processing site at aa 117. This would produce a mature SorCS2 protein of 1042 aa with a 961 aa extracellular/lumenal domain (ECD), a 21 aa transmembrane domain and a 60 aa cytoplasmic domain. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain (2). Within the ECD, mouse SorCS2 shares 98%, 89%, 85% and 76% aa identity with rat, human, equine and canine SorCS2, respectively. It also shares ~46% aa identity with the ECD of either SorCS1 or SorCS3. Unlike other SorCS, shedding of the SorCS2 ECD occurs very slowly and is mainly independent of the metalloproteinase TACE/ADAM17 (4).

1. Hampe, W. et al. (2001) Hum. Genet. 108:529.
2. Rezgaoui, M. et al. (2001) Mech. Dev. 100:335.
3. Hermey, G. et al. (2004) J. Neurochem. 88:1470.
4. Hermey, G. et al. (2006) Biochem. J. 395:285.
5. Westergaard, U. et al. (2004) J. Biol. Chem. 279:50221.
6. Westergaard, U. B. et al. (2005) FEBS Lett. 579:1172.

Bioinformatics

• Gene Symbol: Sorcs2
• Uniprot:
  • Q9EPR5()