Recombinant Mouse Serum Amyloid A2 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse Serum Amyloid A2 Protein, CF Summary

Details of Functionality
Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 1.5-7.5 μg/mL.
Source
E. coli-derived mouse Serum Amyloid A2 protein
Gly20-Tyr122
Accession #
N-terminal Sequence
Gly20
Protein/Peptide Type
Recombinant Proteins
Gene
Saa2
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
11 kDa, reducing conditions
Publications
Read Publication using
8478-SA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris.
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Serum Amyloid A2 Protein, CF

  • SAA
  • SAA2
  • Serum Amyloid A2

Background

Mouse Serum Amyloid A2 (SAA2) is a multifunctional apolipoprotein produced by hepatocytes in response to pro-inflammatory cytokines (1, 2). It is secreted as a 12 kDa, non-glycosylated protein and circulates as part of the HDL complex (3, 4). The SAA2 gene is one of five SAA genes in mouse (5). SAA2 and SAA1 are categorized as acute phase (A)-SAA proteins based on their structural similarity and inducible expression during chronic inflammation. Mature mouse SAA2 shares 70% amino acid (aa) sequence identity with human SAA2 and 92% sequence identity with mouse SAA1. Secretion of SAA2 is increased during inflammation with amounts of total A-SAA increasing up to 1000-fold (6, 7). Increased levels of A-SAA in serum are indicative of inflammatory disease (8). When highly expressed, SAA can displace ApoA1 as the major apolipoprotein in HDL complexes, weakening the function of HDL as a reverse (lipid clearing) cholesterol transporter (2). A highly charged region of SAA2 and SAA1 (aa 36-68) contains putative fibronectin- and laminin-binding motifs (5). This region also binds heparin sulfate proteoglycans at mildly acidic pH and promotes aggregation of A-SAA (9, 10). Persistent production of A-SAA results in amyloid A amyloidosis, a chronic inflammatory condition that culminates in renal failure (10-12). Amyloidosis is triggered by tissue damage, infection, or other insults that result in prolonged inflammatory cytokine activity. SAA2 selectively accumulates in amyloid fibril deposits during amyloidosis. Circulating levels of total A-SAA decrease during active amyloidosis due to the sequestration of SAA2 into amyloid depositions (13, 14). Mouse strains can differ in SAA sequence, expression and amyloid formation (15).

  1. Thorn, C.F. and A.S. Whitehead (2002) Amyloid 9:229.
  2. Benditt, E.P. et al. (1979) Proc. Natl. Acad. Sci. U.S.A. 76:4092.
  3. Hoffman, J.S. et al. (1984) J. Exp. Med. 159:641.
  4. Uhlar, C.M. and A.S. Whitehead (1999) Eur. J. Biochem. 265:501.
  5. van der Westhuyzen, D.R. et al. (2007) Curr. Opin. Lipidol. 18:147.
  6. Hebert, L. and F. Gervais (1990) Scand. J. Immunol. 31:167.
  7. Kluve-Beckerman, B. et al. (1997) Biochem. J. 322(Pt 2):663.
  8. Lu, J. et al. (2014) Proc. Natl. Acad. Sci. U.S.A. 111:5189.
  9. Elimova, E. et al. (2009) FASEB J. 23:3436.
  10. Yu, J. et al. (2000) Lab. Invest. 80:1797.
  11. Jaffe, R.H. (2001) Arch. Pathol. Lab. Med. 125:25.
  12. Yu, J. et al. (2000) Amyloid 7:32.
  13. Meek, R.L. et al. (1986) J. Exp. Med. 163:499.
  14. Simons, J.P. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110:16115.
  15. Mori, M. et al. (2014) Exp. Anim. 63:99.

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Publications for SAA2 (8478-SA)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol Saa2
Uniprot