Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 5 μg/mL of Recombinant Mouse Semaphorin 6B Fc Chimera causes >50% growth cone collapse in the presence of 10 ng/mL of Recombinant Human beta -NGF (Catalog # 256-GF).
• Source: Human embryonic kidney cell, HEK293-derived mouse Semaphorin 6B protein
  

  Mouse Semaphorin 6B (Leu27-Ser605) Accession # O54951	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: O54951
• N-terminal Sequence: Leu27
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Sema6b
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 91 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-115 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Tween® 20.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF

• sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B
• Sema6B
• SEMAN
• Semaphorin 6B
• semaphorin VIB
• semaphorin Z
• semaphorin-6B
• semaphorin-6Ba
• SEMA-VIB
• SEMAZ
• SEM-SEMA-Y
• UNQ1907/PRO4353

Background

Semaphorin 6B is a member of the class 6 subfamily of semaphorins, a large, highly conserved family of signaling molecules that affect multiple processes including axon guidance, cell migration, synaptogenesis, and dendritic spine formation (1). The class 6 semaphorins are type I transmembrane glycoproteins that contain the characteristic extracellular beta propeller-structured N-terminal semaphorin (sema) domain and also an extracellular plexin-semaphorin-integrin (PSI) domain (2, 3). Additionally, the cytoplasmic domain of Semaphorin 6B contains a proline-rich sequence that has been shown to interact with the SH3 domain of the Src signaling protein (4). Mouse Semaphorin 6B has a molecular weight of approximately 120 kDa and is thought to form disulfide-linked homodimers (4). It shares 95% and 99% amino acid (aa) sequence identity with the human and rat orthologs, respectively. In mice, Semaphorin 6B is highly expressed in developing muscle and in subregions of the nervous system, as well as at low levels in most adult tissues (4). It has been shown to mediate repulsion of mouse sympathetic ganglion axons and hippocampal mossy fibers via binding to the transmembrane protein Plexin A4 (5, 6). Semaphorin 6B has also been shown to be highly expressed in human gastric, breast, and glioblastoma cancer cells and is thought to be involved in tumor differentiation and metastasis (7-9).

1. Pasterkamp, R.J. and R.J. Giger (2009) Curr. Opin. Neurobiol. 19:263.
2. Neufeld, G. et al. (2005) Front. Biosci. 10:751.
3. Roney, K. et al. (2013) Protein Cell 4:17.
4. Eckhardt, F. et al. (1997) Mol. Cell. Neurosci. 9:409.
5. Suto, F. et al. (2005) J. Neurosci. 25:3628.
6. Tawarayama, H. et al. (2010) J. Neurosci. 30:7049.
7. Correa, R.G. et al. (2001) Genomics 73:343.
8. Murad, H. et al. (2006) Int. J. Oncol. 28:977.
9. Ge, C. et al. (2013) J. Int. Med. Res. 41:284.

Bioinformatics

• Gene Symbol: Sema6b
• Uniprot:
  • O54951()