Recombinant Mouse RGM-A Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Mouse RGM-A Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human BMP-4 (Catalog # 314-BP) is coated at 1 μg/mL (100 μL/well), Recombinant Mouse RGM-A binds with an apparent Kd <50 nM.
Source
Mouse myeloma cell line, NS0-derived mouse RGM-A protein
Mouse RGM-A
Cys48-Gly421 &
Pro170-Gly421
Accession # Q6PCX7
DI 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Cys48 (proprotein) & Pro170 (mature)
Protein/Peptide Type
Recombinant Proteins
Gene
Rgma
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
42.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
Multiple bands between 25-37 kDa, reducing conditions
Publications
Read Publications using
2458-RG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse RGM-A Protein, CF

  • RGM domain family member A
  • RGM domain family, member A
  • RGMA
  • RGM-A
  • RGMrepulsive guidance molecule A

Background

Mouse repulsive guidance molecule (RGM‑A) is a 33 kDa GPI-linked member of an expanding RGM‑related family of neuronal and muscle-expressed membrane proteins (1, 2). It is synthesized as a 454 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 122 aa N-terminal prosegment, a 258 mature region (aa 170-427) and a 27 aa C-terminal prosegment (3, 4). The N-terminal prosegment contains an RGD tripeptide and the molecule's only two potential N‑linked glycosylation sites. The mature segment shows an abbreviated von Willebrand factor domain. Proteolytic processing occurs at an aspartic acid‑proline bond, creating a predicted 32 kDa mature region (3). There is one potential alternative start site at Met17. The mature region of mouse RGM-A is 93% and 87% aa identical to human and chick mature region RGM-A, respectively. Recognizing that RGM-A possesses a cleavage site between Asp169Pro170, it is suggested that membrane-expressed RGM-A may exist as a covalent heterodimer that links a GPI-anchored mature molecule with its prosegment (1, 4, 5). When compared to mouse RGM-B and C, the mature region of mouse RGM-A shows 77% and 76% aa identity, respectively (5, 6). Cells normally described as expressing RGM-A include neurons and oligodendroglia. But this select group is expanded to endothelium, microglia and leukocytes in areas of neuronal injury (7). Functionally, recombinant chick RGM has been reported to induce collapse of temporal but not nasal growth cones, and to repel temporal retinal axons in vitro. This suggests a role in the development of the retina-superior colliculus connection. In mice, RGM-A also induces growth cone collapse on primary DRG neurons (8). And in a mouse culture system, chick RGM-A is reported to be responsible for the layered segmentation of entorhinal cortical projections to the hippocampus (9). RGM reportedly binds to neogenin and BMP-2/4, with the latter binding occuring within the context of a BMPRI:ActRIIA:RGM-A receptor complex (10-13).

  1. Severyn, C.J. et al. (2009) Biochem. J. 422:393.
  2. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
  3. Schmidtmer, J. et al. (2004) Gene Expr. Patterns 4:105.
  4. Matsunaga, E. & A. Chedotal (2004) Develop. Growth Differ. 46:481.
  5. Monnier, P.P. et al. (2002) Nature 419:392.
  6. Niederkofler V. et al. (2004) J. Neurosci. 24:808.
  7. Hata, K. et al. (2006) J. Cell Biol. 173:47.
  8. Conrad, S. et al. (2007) J. Biol. Chem. 282:16423.
  9. Brinks, H. et al. (2004) J. Neurosci. 24:3862.
  10. Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.
  11. Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.
  12. Babitt, J.L. et al. (2005) J. Biol. Chem. 280:29820.
  13. Xia, Y. et al. (2007) J. Biol. Chem. 282:18129.

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Publications for RGM-A (2458-RG)(5)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: Bioassay, ELISA (Capture).


Filter By Application
Bioassay
(4)
ELISA (Capture)
(1)
All Applications
Filter By Species
Mouse
(4)
Rat
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using 2458-RG Applications Species
Xiao, Yanping, Yu, Sanhong, Zhu, Baogong, Bedoret, Denis, Bu, Xia, Francisco, Loise M, Hua, Ping, Duke-Cohan, Jonathan, Umetsu, Dale T, Sharpe, Arlene H, DeKruyff, Rosemari, Freeman, Gordon J RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance. J Exp Med, 2014;211(5):943-59. 2014 [PMID: 24752301] (ELISA (Capture), Mouse) ELISA (Capture) Mouse
O'Leary, Conor, Cole, Stacey J, Langford, Michael, Hewage, Jayani, White, Amanda, Cooper, Helen M RGMa regulates cortical interneuron migration and differentiation. PLoS ONE, 2013;8(11):e81711. 2013 [PMID: 24312340] (Bioassay, Mouse) Bioassay Mouse
Liu X, Hashimoto M, Horii H, Yamaguchi A, Naito K, Yamashita T Repulsive guidance molecule b inhibits neurite growth and is increased after spinal cord injury. Biochem. Biophys. Res. Commun., 2009;382(4):795-800. 2009 [PMID: 19324014] (Bioassay, Rat) Bioassay Rat
Schaffar G, Taniguchi J, Brodbeck T, Meyer AH, Schmidt M, Yamashita T, Mueller BK LIM-only protein 4 interacts directly with the repulsive guidance molecule A receptor Neogenin. J. Neurochem., 2008;107(2):418-31. 2008 [PMID: 18702663] (Bioassay, Mouse) Bioassay Mouse
Suda M, Hata K, Sawada A, Nakamura Y, Kubo T, Yamaguchi A, Yamashita T Peptides derived from repulsive guidance molecule act as antagonists. Biochem. Biophys. Res. Commun., 2008;371(3):501-4. 2008 [PMID: 18452705] (Bioassay, Mouse) Bioassay Mouse

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Bioinformatics

Gene Symbol Rgma
Uniprot