Recombinant Mouse R-Spondin 1 Protein, CF


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Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse R-Spondin 1 Protein, CF Summary

Additional Information
A New rm R-Spondin 1 is Available! CHO expressed; ~5 fold better activity; Enhanced purity!
Details of Functionality
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
E. coli-derived mouse R-Spondin 1 protein
Ser21-Gly209, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
3474-RS in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse R-Spondin 1 Protein, CF

  • Cristin 3
  • FLJ40906Roof plate-specific spondin-1
  • HRspo1
  • roof plate-specific spondin
  • RSPO
  • RSPO1
  • RSpondin 1
  • R-Spondin 1
  • R-spondin homolog (Xenopus laevis)
  • R-spondin1
  • R-spondin-1


R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Mutations in R-Spondin 1 are also linked with palmoplantar keratoderma, abnormal thickening of the skin on the palms of the hands and soles of the feet (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (11).

Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 12). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (13). Mouse R‑Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively, within aa 21‑209.

  1. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
  2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
  3. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  4. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  5. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  6. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
  7. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
  8. Parma, P. et al. (2006) Nat. Genet. 38:1304.
  9. Kim, K.-A. et al. (2005) Science 309:1256.
  10. Zhao, J. et al. (2007) Gastroenterology 132:1331.
  11. Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
  12. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
  13. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.

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Publications for R-Spondin 1 (3474-RS)(43)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: Bioassay, Cell Culture, IHC Control, In Vivo, Organoid Culture, TOPflash assay, Tissue Culture.

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IHC Control
In Vivo
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Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using 3474-RS Applications Species
M Zhang, M Haughey, NY Wang, K Blease, AM Kapoun, S Couto, I Belka, T Hoey, M Groza, J Hartke, B Bennett, J Cain, A Gurney, B Benish, P Castiglion, C Drew, J Lachowicz, L Carayannop, SD Nathan, J Distler, DA Brenner, K Hariharan, H Cho, W Xie Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs PLoS ONE, 2020;15(3):e0229445. 2020 [PMID: 32160239] (IHC Control, Mouse) IHC Control Mouse
T Xing, LJ Benderman, S Sabu, J Parker, J Yang, Q Lu, L Ding, YH Chen Tight junction protein claudin-7 is essential for intestinal epithelial stem cell self-renewal and differentiation Cell Mol Gastroenterol Hepatol, 2019;0(0):. 2019 [PMID: 31874254] (Organoid Culture, Mouse) Organoid Culture Mouse
AP Singh, YH Hung, MT Shanahan, M Kanke, A Bonfini, MK Dame, M Biraud, BCE Peck, OO Oyesola, JM Freund, RL Cubitt, EG Curry, LM Gonzalez, GA Bewick, ED Tait-Wojno, NA Kurpios, S Ding, JR Spence, CM Dekaney, N Buchon, P Sethupathy Enteroendocrine progenitor cell enriched miR-7 regulates intestinal epithelial proliferation in an Xiap-dependent manner Cell Mol Gastroenterol Hepatol, 2019;0(0):. 2019 [PMID: 31756561] (Tissue Culture, Mouse) Tissue Culture Mouse
X Wang, Y Han, M Wang, C Bo, Z Zhang, L Xu, W Liu, H Wang Wnt Signaling Protects against Paclitaxel-Induced Spiral Ganglion Neuron Damage in the Mouse Cochlea In Vitro Biomed Res Int, 2019;2019(0):7878906. 2019 [PMID: 31687397] (Tissue Culture, Mouse) Tissue Culture Mouse
T Yung, F Poon, M Liang, S Coquenlorg, EC McGaugh, CC Hui, MD Wilson, MC Nostro, TH Kim Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals Nat Commun, 2019;10(1):4647. 2019 [PMID: 31604927] (Bioassay, Mouse) Bioassay Mouse
EM Schatoff, S Goswami, MP Zafra, M Foronda, M Shusterman, BI Leach, A Katti, BJ Diaz, LE Dow Distinct CRC-associated APC mutations dictate response to Tankyrase inhibition Cancer Discov, 2019;0(0):. 2019 [PMID: 31337618] (Tissue Culture, Mouse) Tissue Culture Mouse
KK Dickinson, LC Hammond, CM Karner, ND Hastie, TJ Carroll, P Goodyer Molecular determinants of WNT9b responsiveness in nephron progenitor cells PLoS ONE, 2019;14(4):e0215139. 2019 [PMID: 30978219] (TOPflash assay, Mouse) TOPflash assay Mouse
Z Meng, T Gwag, Y Sui, SH Park, X Zhou, C Zhou The atypical antipsychotic quetiapine induces hyperlipidemia by activating intestinal PXR signaling JCI Insight, 2019;4(3):. 2019 [PMID: 30728326] (Bioassay, Mouse) Bioassay Mouse
F Chen, W Yang, X Huang, AT Cao, AJ Bilotta, Y Xiao, M Sun, L Chen, C Ma, X Liu, CG Liu, S Yao, SM Dann, Z Liu, Y Cong Neutrophils Promote Amphiregulin Production in Intestinal Epithelial Cells through TGF-? and Contribute to Intestinal Homeostasis J. Immunol., 2018;0(0):. 2018 [PMID: 30171165] (Bioassay, Mouse) Bioassay Mouse
NR Smith, JR Swain, PS Davies, AC Gallagher, MS Parappilly, CZ Beach, PR Streeter, IA Williamson, ST Magness, MH Wong Monoclonal Antibodies Reveal Dynamic Plasticity Between Lgr5-�and Bmi1-Expressing Intestinal Cell Populations Cell Mol Gastroenterol Hepatol, 2018;6(1):79-96. 2018 [PMID: 29928673] (Bioassay, Mouse) Bioassay Mouse
Show All 43 Publications.

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Gene Symbol Rspo1