Recombinant Mouse Pref-1/DLK-1/FA1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Pref-1/DLK-1/FA1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Mouse Pref‑1/DLK1/FA1 (Catalog # 8545-PR) binds Mouse Pref-1/DLK1/FA1 Antibody (Catalog # MAB8634) with an ED₅₀ of 0.180-1.80 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Pref-1/DLK1/FA1 protein
  Ala24-Gln305, with a C-terminal 6-His tag
• Accession #: Q09163
• N-terminal Sequence: Ala24
• Protein/Peptide Type: Recombinant Proteins
• Gene: Dlk1
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 49-66 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Pref-1/DLK-1/FA1 Protein, CF

• delta-like 1 homolog (Drosophila)
• DLK
• DLK1
• DLK-1
• FA1
• FA1fetal antigen 1
• pG2
• pG2delta-like homolog (Drosophila)
• preadipocyte factor 1
• Pref1
• Pref-1
• protein delta homolog 1
• secredeltin
• ZOG

Background

Mouse Pref-1 (preadipocyte factor 1), also known as Dlk-1 or FA1, belongs to the Notch/Delta/Serrate family of EGF-like repeat-containing proteins that act as ligands in the Notch signaling pathway (1-3). Mouse Pref-1 is synthesized as a 385 amino acid (aa) precursor consisting of a 23 aa signal sequence, a 282 aa extracellular region, a 24 aa transmembrane segment, and a 56 aa cytoplasmic tail. The extracellular region contains six tandem EGF-like repeats, a juxtamembrane region, and multiple sites for O- and N-glycosylation. Heterogeneous transmembrane forms of Pref-1, ranging from 50 to 60 kDa, can be attributed to variability in glycosylation (2-4). In addition to full-length Pref-1A, alternative splicing of the juxtamembrane and EGF-like repeat regions generates three major short forms, Pref-1B, -1C, and
-1D (5). Proteolytic cleavage at one of two extracellular sites in Pref-1A and 1B can produce two soluble forms: a 50 kDa large form and a 24-25 kDa small form (2, 5, 6). Processing of Pref-1C and Pref-1D, by contrast, can produce only small soluble forms. Only the large soluble form demonstrates biological activity (5). The ECD of mature mouse Pref-1 shares 94% and 83% aa sequence identity with the ECD of human and rat Pref-1, respectively. Pref-1 is widely expressed during embryogenesis (2). In adults, Pref-1 expression is restricted to preadipocytes, pancreatic beta  cells, thymic stromal cells, and cells of the adrenal and pituitary glands
(1, 7-9). Tumors of neuroendocrine origin have been found to express Pref-1 (10). Down-regulation of Pref-1 in preadipocytes is associated with differentiation into mature adipocytes (1-7). Pref-1 also regulates the differentiation of skeletal stem cells, thymocytes, and adrenal gland cells and inhibits hormone secretion in pituitary cells (1). Pref-1 has also been shown to inhibit preadipocyte proliferation (11, 12).

1. Ansell, P.J. et al. (2007) Mol. Cell. Endocrinol. 271:55.
2. Smas, C.M. and H.S. Sul (1993) Cell 73:725.
3. Sul, H.S. (2009) Mol. Endocrinol. 23:1717.
4. Smas, C.M. et al. (1994) Biochemistry 33:9257.
5. Mei, B. et al. (2002) Biochem. J. 364:137.
6. Wang, Y. and H.S. Sul (2006) Mol. Cell. Biol. 26:5421.
7. Wang, Y. et al. (2010) Mol. Cell. Biol. 30:3480.
8. Friedrichsen, B.N. et al. (2003) J. Endocrinol. 176:257.
9. Hedlund, G.P. et al. (2003) In Vivo 17:1.
10. Yin, D. et al. (2006) Oncogene 25:1852.
11. Traustadottir, G.A. et al. (2013) Adipocyte 2:272.
12. Mortensen, S.B. et al. (2012) Diabetes 61:2814.

Bioinformatics

• Gene Symbol: Dlk1
• Uniprot:
  • Q09163()