Recombinant Mouse Podoplanin Fc Chimera Protein, CF Summary
Details of Functionality
Measured by its binding ability in a functional ELISA. When 0.5 μg/mL of rmPodoplanin/Fc is immobilized onto a Goat Anti-Mouse IgG Fc chimera (R&D Systems, Catalog # G-202-C) coated plate, the concentration of rhCLEC-2 that produces 50% of the optimal binding response is found to be approximately 0.3-1.8 nM.
Mouse myeloma cell line, NS0-derived mouse Podoplanin protein
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.01 EU per 1 μg of the protein by the LAL method.
19.0 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Podoplanin, also known as glycoprotein 38 (gp38), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 38 kDa type I transmembrane sialoglycoprotein and member of the podoplanin family (1 - 2). Podoplanin is synthesized as a 172 amino acid (aa) precursor with a 22 aa signal sequence, a 119 aa extracellular domain (ECD), a 21 aa transmembrane region, and a short, 10 aa cytoplasmic tail (SwissProt #: Q62011). The ECD contains abundant Ser/Thr residues as potential sites for O-glycosylation, and the cytoplasmic region contains putative sites for kinase C and cAMP phosphorylation (2 - 3). Mouse Podoplanin shares 77% and 46% aa sequence identity with rat and human Podoplanin, respectively. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells (4 - 5), and on numerous tumors including colorectal tumors (3), squamous cell carcinomas (4, 6), testicular seminoma (7), and brain tumors (8 - 10). One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver (1). Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2) (2). Their association is dependent on sialic acid on O-glycans of Podoplanin (2). Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis (2). Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth (2).
Zimmer, G. et al. (1999) Biochem. J. 341:277.
Katsue-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
Kato, Y. et al. (2003) J. Biol. Chem. 278:51599.
Schacht, V. et al. (2005) Am. J. Pathol. 166:913.
Breiteneder-Geleff, S. et al. (1997) Am. J. Pathol. 151:1141.
Kato, Y. et al. (2005) Tumour Biol. 26:195.
Kato, Y. et al. (2004) Oncogene 23:8552.
Mishima, K. et al. (2006) Acta Neuropathol. 111:563.
Mishima, K. et al. (2006) Acta Neuropathol. 111:483.
Kato, Y. et al. (2006) Biochem. Biophys. Res. Commun. 349:1301.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Recombinant Mouse Podoplanin Fc Chimera Protein, CF and receive a gift card or discount.