Recombinant Mouse Nodal Protein, CF


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Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse Nodal Protein, CF Summary

Details of Functionality
Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Yeo, C. et al. (2001) Mol. Cell. 7:949. Approximately 100 ng/mL of Recombinant Mouse Nodal can effictively induce Smad2 phosphorylation.
E. coli-derived mouse Nodal protein
His245-Leu354, with an N-terminal Met
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
13 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
1315-ND/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Nodal Protein, CF

  • BMP-16
  • MGC138230
  • nodal homolog (mouse)
  • nodal homolog
  • Nodal
  • nodal, mouse, homolog


Nodal is a secreted protein that is a member of the Transforming Growth Factor-beta (TGF-beta ) superfamily. Nodal was named for its localized expression in the mouse node during gastrulation, and is first detected in the early primitive streak during mesoderm formation. Expression of the Nodal gene occurs asymmetrically in the left, but not right, lateral plate during somitogenesis. Nodal proteins play crucial roles in mesoderm formation and both anterior-posterior and left-right axis formation during vertebrate development. Members of the Nodal gene family include mouse Nodal, chick cNR-1, frog Xnr1-4, and zebrafish cyclops. Biologically active Nodal is a disulfide-linked homodimer and contains all seven of the cysteine residues necessary for formation of the “cysteine knot” characteristic of TGF-beta -related molecules. Mouse Nodal is 34 - 39% homologous in the conserved region to other TGF-beta superfamily members. Nodal has been shown to signal through a mechanism related to the Activin pathway, and signaling is mediated through both Smad2 and 3. Nodal signaling utilizes type II Activin receptors, together with ALK4/ActRIB, or the orphan type I receptor ALK7. Nodal interacts extracellularly with members of other protein families, including Cerberus, Lefty, and EGF-CFC ligands, such as Cripto. While the Cerberus and Lefty families act as Nodal antagonists, the EGF-CFC molecules act as co-receptors to facilitate Nodal signaling. The resulting concert of regulated Nodal activity allows for the precise control of mesoderm formation, neural patterning, and axis positioning and patterning during early vertebrate development.

  1. Kumar, A. et al. (2001) J. Biol. Chem. 276:656.
  2. Reissmann, E. et al. (2001) Genes & Dev. 15: 2010.
  3. Schier, A. and M. Shen (1999) Nature 403:385.
  4. Zhou, X. et al. (1993) Nature 361:543.

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Publications for Nodal (1315-ND/CF)(12)

We have publications tested in 5 confirmed species: Human, Mouse, N/A, Xenopus, Zebrafish.

We have publications tested in 3 applications: Bioassay, IHC absorption control, In Vivo.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 1315-ND/CF Applications Species
P Scerbo, L Marchal, L Kodjabachi Lineage commitment of embryonic cells involves MEK1-dependent clearance of pluripotency regulator Ventx2 Elife, 2017;6(0):. 2017 [PMID: 28654420] (In Vivo, Xenopus) In Vivo Xenopus
Dubrulle, Julien, Jordan, Benjamin, Akhmetova, Laila, Farrell, Jeffrey, Kim, Seok-Hyu, Solnica-Krezel, Lilianna, Schier, Alexande Response to Nodal morphogen gradient is determined by the kinetics of target gene induction. Elife, 2015;4(0):. 2015 [PMID: 25869585] (In Vivo, Zebrafish) In Vivo Zebrafish
Jahn S, Law M, Corsino P, Davis B, Harrison J, Law B Signaling mechanisms that suppress the cytostatic actions of rapamycin. PLoS ONE, 2014;9(6):e99927. 2014 [PMID: 24927123] (Bioassay, Mouse) Bioassay Mouse
Fuller K, O'Connell J, Gordon J, Mauti O, Eggenschwiler J Rab23 regulates Nodal signaling in vertebrate left-right patterning independently of the Hedgehog pathway. Dev Biol, 2014;391(2):182-95. 2014 [PMID: 24780629] (In Vivo, Mouse) In Vivo Mouse
Kumari, Pooja, Gilligan, Patrick, Lim, Shimin, Tran, Long Duc, Winkler, Sylke, Philp, Robin, Sampath, Karuna An essential role for maternal control of Nodal signaling. Elife, 2013;2(0):e00683. 2013 [PMID: 24040511] (In Vivo, Zebrafish) In Vivo Zebrafish
Souquet B, Tourpin S, Messiaen S, Moison D, Habert R, Livera G Nodal signaling regulates the entry into meiosis in fetal germ cells. Endocrinology, 2012;153(5):2466-73. 2012 [PMID: 22396454] (Bioassay, Mouse) Bioassay Mouse
Hansson M, Olesen DR, Peterslund JM, Engberg N, Kahn M, Winzi M, Klein T, Maddox-Hyttel P, Serup P A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. Dev. Biol., 2009;330(2):286-304. 2009 [PMID: 19358838] (Bioassay, Mouse) Bioassay Mouse
Borowiak M, Maehr R, Chen S, Chen AE, Tang W, Fox JL, Schreiber SL, Melton DA Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell, 2009;4(4):348-58. 2009 [PMID: 19341624] (Bioassay, Human) Bioassay Human
Levine AJ, Levine ZJ, Brivanlou AH GDF3 is a BMP inhibitor that can activate Nodal signaling only at very high doses. Dev. Biol., 2009;325(1):43-8. 2009 [PMID: 18823971] (Bioassay, Xenopus) Bioassay Xenopus
Oki S, Hashimoto R, Okui Y, Shen MM, Mekada E, Otani H, Saijoh Y, Hamada H Sulfated glycosaminoglycans are necessary for Nodal signal transmission from the node to the left lateral plate in the mouse embryo. Development, 2007;134(21):3893-904. 2007 [PMID: 17913787] (Bioassay, Mouse) Bioassay Mouse
Show All 12 Publications.

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Gene Symbol Nodal