CRIPTO Antibody

Images

 
Western Blot: CRIPTO Antibody [NB100-1598] - Analysis of Cripto in Dicer knockout mouse embryonic stem cell lysate.
Immunocytochemistry/ Immunofluorescence: CRIPTO Antibody [NB100-1598] - CRIPTO antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on HeLa cells with CRIPTO Antibody NB100-1598PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Flow Cytometry: CRIPTO Antibody [NB100-1598] - Staining of NTERA-2 cells using NB100-1598 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CRIPTO Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal portion of mouse Cripto1 (between residues 1-50). [UniProt# P51865]
Localization
Membrane-bound
Marker
Embryonic Stem Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TDGF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:1000
Application Notes
This Cripto1 antibody is useful in Immunocytochemistry/Immunofluorescence, Flow Cytometry and Western Blot. In Western blot a band is seen at ~18 kDa representing the core form of Cripto1, and at ~36 kDa reporesenting the glycosylated/ post-translationally modified form. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
CRIPTO Peptide (NB100-1598PEP)
Publications
Read Publications using
NB100-1598 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CRIPTO Antibody

  • CFC-2
  • CR
  • Cripto
  • Cripto-1 growth factor
  • Cripto-1
  • CRIPTOCRGF
  • Epidermal growth factor-like cripto protein CR1
  • TDGF1
  • TDGF-1
  • teratocarcinoma-derived growth factor 1

Background

Cripto-1 (teratocarcinoma-derived growth factor-1) is the original member of EGF-cripto-1-FRL-1-cryptic (CFC) family of vertebrate signaling molecules which is essential for embryonic development and it implicates in carcinogenesis also. Cripto1 functions as a co-receptor for NODAL (TGF-beta member) and is essential for mesoderm as well as endoderm formation and anterior-posterior and left-right axis establishment. After executing key functions during embryonic development, it dramatically disappears in adult tissues (except stem cells). Cripto-1 modulate signaling of TGF-beta family members (NODAL, GDF-1/-3, Activin, TGF-beta1 etc), and activate c-src/MAPK/AKT pathway in a Glypican-1/GRP78-dependent manner, and cross-talks with erbB4, Wnt/beta-catenin, NOTCH, Caveolin-1, and Apelin/putative receptor protein related to angiotensin-type I receptor pathways. During embryonic development, Cripto-1 together with Nodal controls cell migration and EMT, whereas, postnatally, it regulates the branching morphogenesis of mammary gland. Moreover, in adult life it gets re -expressed in several type of tumors wherein it promotes cell proliferation, migration, invasion, EMT, and angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CRIPTO Antibody (NB100-1598)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Reviews for CRIPTO Antibody (NB100-1598) (0)

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Product General Protocols

View specific protocols for CRIPTO Antibody (NB100-1598): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CRIPTO Antibody (NB100-1598). (Showing 1 - 1 of 1 FAQs).

  1. We would like to check toxicity of following products. For the following antibodies, could you inform us the concentration of sodium azide? Synaptojanin 1 Antibody (AC1) (cat# NB300-428), Ly-6C Antibody (HK1.4) [Biotin] (cat# NBP1-28048), and Cripto1 Antibody (cat# NB100-1598). For your MCF-7 Whole Cell Lysate (cat# NB820-59465), could you inform us the concentration of EDTA, Glycerol and NP-40?
    • NB300-428 is supplied in 0.02% Sodium Azide NBP1-28048 contains 0.1% Sodium Azide and NB100-1598 has 0.05% sodium azide. With regards to your second question, unfortunately the formula for the lysis buffer is proprietary thus the component concentration is not suppose to be disclosed due to Novus policy.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-1598AF405
Alexa Fluor 488 Labeled NB100-1598AF488
Alexa Fluor 647 Labeled NB100-1598AF647
Alexa Fluor 700 Labeled NB100-1598AF700
Biotin Labeled NB100-1598B
DyLight 405 Labeled NB100-1598V
DyLight 488 Labeled NB100-1598G
DyLight 550 Labeled NB100-1598R
DyLight 650 Labeled NB100-1598C
FITC Labeled NB100-1598F
HRP Labeled NB100-1598H
PE Labeled NB100-1598PE

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Bioinformatics Tool for CRIPTO Antibody (NB100-1598)

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Bioinformatics

Gene Symbol TDGF1
Entrez