CRIPTO Antibody - BSA Free


Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% more
Immunocytochemistry/ Immunofluorescence: CRIPTO Antibody [NB100-1598] - CRIPTO antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 more
Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on Jurkat with NB100-1598 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells more
Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on HeLa cells with NB100-1598PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF
BSA Free
1.0 mg/ml

Order Details

CRIPTO Antibody - BSA Free Summary

A synthetic peptide made to an N-terminal portion of mouse Cripto1 (between residues 1-50). [UniProt# P51865]
Embryonic Stem Cell Marker
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Western Blot 2 ug/ml
Application Notes
This Cripto1 antibody is useful in Immunocytochemistry/Immunofluorescence, Flow Cytometry and Western Blot. In Western blot a band is seen at ~18 kDa representing the core form of Cripto1, and at ~36 kDa reporesenting the glycosylated/ post-translationally modified form. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
CRIPTO Peptide (NB100-1598PEP)
Read Publications using
NB100-1598 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for CRIPTO Antibody - BSA Free

  • CFC-2
  • CR
  • Cripto
  • Cripto-1 growth factor
  • Cripto-1
  • Epidermal growth factor-like cripto protein CR1
  • TDGF1
  • TDGF-1
  • teratocarcinoma-derived growth factor 1


Cripto-1 (teratocarcinoma-derived growth factor-1) is the original member of EGF-cripto-1-FRL-1-cryptic (CFC) family of vertebrate signaling molecules which is essential for embryonic development and it implicates in carcinogenesis also. Cripto1 functions as a co-receptor for NODAL (TGF-beta member) and is essential for mesoderm as well as endoderm formation and anterior-posterior and left-right axis establishment. After executing key functions during embryonic development, it dramatically disappears in adult tissues (except stem cells). Cripto-1 modulate signaling of TGF-beta family members (NODAL, GDF-1/-3, Activin, TGF-beta1 etc), and activate c-src/MAPK/AKT pathway in a Glypican-1/GRP78-dependent manner, and cross-talks with erbB4, Wnt/beta-catenin, NOTCH, Caveolin-1, and Apelin/putative receptor protein related to angiotensin-type I receptor pathways. During embryonic development, Cripto-1 together with Nodal controls cell migration and EMT, whereas, postnatally, it regulates the branching morphogenesis of mammary gland. Moreover, in adult life it gets re -expressed in several type of tumors wherein it promotes cell proliferation, migration, invasion, EMT, and angiogenesis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CRIPTO Antibody (NB100-1598)(3)

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Product General Protocols

View specific protocols for CRIPTO Antibody (NB100-1598): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CRIPTO Antibody (NB100-1598). (Showing 1 - 1 of 1 FAQs).

  1. We would like to check toxicity of following products. For the following antibodies, could you inform us the concentration of sodium azide? Synaptojanin 1 Antibody (AC1) (cat# NB300-428), Ly-6C Antibody (HK1.4) [Biotin] (cat# NBP1-28048), and Cripto1 Antibody (cat# NB100-1598). For your MCF-7 Whole Cell Lysate (cat# NB820-59465), could you inform us the concentration of EDTA, Glycerol and NP-40?
    • NB300-428 is supplied in 0.02% Sodium Azide NBP1-28048 contains 0.1% Sodium Azide and NB100-1598 has 0.05% sodium azide. With regards to your second question, unfortunately the formula for the lysis buffer is proprietary thus the component concentration is not suppose to be disclosed due to Novus policy.

Secondary Antibodies


Isotype Controls

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Gene Symbol TDGF1