Recombinant Mouse Neurturin Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity, Bioactivity

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Recombinant Mouse Neurturin Protein Summary

Details of Functionality
Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. Davies, A.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R.A. (eds): John Willey and Sons, Ltd. 95.

The ED50 for this effect is 2-10 ng/mL.

Also measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells.

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse GFR alpha ‑2/GDNF R alpha ‑2 Fc Chimera (Catalog # 429-FR) at 1 µg/mL can bind Recombinant Mouse Neurturin with an apparent KD <3.0 nM.
Source
E. coli-derived mouse Neurturin protein
Pro96-Val195
Accession #
N-terminal Sequence
Pro96
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Nrtn
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
11.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
477-MN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Neurturin Protein

  • Neurturin
  • NRTN
  • NTN

Background

Neurturin is a member of the GDNF family of ligands, which include glial cell-derived neurotrophic factor (GDNF), Neurturin, Persephin, and Artemin. GDNF family proteins are distant members of the Transforming Growth Factor beta (TGF-beta ) superfamily (1-4). Similar to other TGF-beta family proteins, Neurturin is synthesized as a precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Neurturin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Biologically active Neurturin is a disulfide-linked homodimer of the carboxy-terminal 100 amino acid residues. Mature mouse Neurturin shares 91% amino acid sequence similarity with human Neurturin. Mature Neurturin also shares about 40% similarities with the other three members of the GDNF family ligands (2, 5). Unlike other members of TGF-beta family, bioactivities of all GDNF family ligands are mediated through a unique multicomponent receptor complex composed of high affinity ligand binding component (GFR alpha -1-GFR alpha -4) and a common signaling component (cRET receptor tyrosine kinase). Each member of the GDNF family ligands has its preferred binding protein. Neurturin preferentially binds to GFR alpha -2 but can also bind GFR alpha -1 at higher concentration (5-8). Neurturin has been shown to promote the survival of a variety of neurons including sympathetic, sensory, and central nervous system neurons. Neurturin is expressed in both neuronal and non-neuronal tissues. It may play a role in regulating the development and maintenance of the central and peripheral nervous systems as well as non-neuronal systems (9).

  1. Lin, L-FH. et al. (1993) Science 260:1130.
  2. Kotzbauer, P.T. et al. (1996) Nature 384:467.
  3. Milbrandt, J. et al. (1998) Neuron 20:245.
  4. Baloh, R.H. et al. (1998) Neuron 21:1291.
  5. Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361.
  6. Baloh, R.H. et al. (1997) Neuron 18:793.
  7. Jing, S. et al. (1996) Cell 85:1113.
  8. Jing, S. et al. (1997) J. Biol. Chem. 272:33111.
  9. Airaksinen, M. and M. Saarma (2002) Nature Review Neuroscience 3:383.

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Publications for Neurturin (477-MN)(7)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.


Filter By Application
Bioassay
(5)
Cell Culture
(1)
In Vivo
(1)
All Applications
Filter By Species
Mouse
(6)
Rat
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using 477-MN Applications Species
JR Cabrera, R Manivanh, BJ North, DA Leib The ESCRT-Related ATPase Vps4 Is Modulated by Interferon during Herpes Simplex Virus 1 Infection MBio, 2019;10(2):. 2019 [PMID: 30837340] (Cell Culture, Mouse) Cell Culture Mouse
S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons Proc. Natl. Acad. Sci. U.S.A, 2017;114(12):E2486-E2493. 2017 [PMID: 28270618] (Bioassay, Mouse) Bioassay Mouse
Rosato P, Leib D Neuronal Interferon Signaling Is Required for Protection against Herpes Simplex Virus Replication and Pathogenesis. PLoS Pathog, 2015;11(7):e1005028. 2015 [PMID: 26153886] (Bioassay, Mouse) Bioassay Mouse
Rosato P, Leib D Intrinsic innate immunity fails to control herpes simplex virus and vesicular stomatitis virus replication in sensory neurons and fibroblasts. J Virol, 2014;88(17):9991-10001. 2014 [PMID: 24942587] (Bioassay, Mouse) Bioassay Mouse
Knox, Sarah M, Lombaert, Isabelle, Haddox, Candace, Abrams, Shaun R, Cotrim, Ana, Wilson, Adrian J, Hoffman, Matthew Parasympathetic stimulation improves epithelial organ regeneration. Nat Commun, 2013;4(0):1494. 2013 [PMID: 23422662] (Bioassay, Mouse) Bioassay Mouse
Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo. J. Neurosci., 2006;26(33):8588-99. 2006 [PMID: 16914685] (In Vivo, Mouse) In Vivo Mouse
Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM Expression and function of GDNF family ligands and receptors in the carotid body. Exp. Neurol., 2005;191(0):S68-79. 2005 [PMID: 15629763] (Bioassay, Rat) Bioassay Rat

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Bioinformatics

Gene Symbol Nrtn
Uniprot