Recombinant Mouse Neurturin Protein

• Reactivity: Mu
• Applications: Binding Activity, Bioactivity

Recombinant Mouse Neurturin Protein Summary

• Details of Functionality: Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. Davies, A.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R.A. (eds): John Willey and Sons, Ltd. 95. The ED₅₀ for this effect is 2-10 ng/mL.
  
  Also measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse GFR alpha ‑2/GDNF R alpha ‑2 Fc Chimera (Catalog # 429-FR) at 1 µg/mL can bind Recombinant Mouse Neurturin with an apparent K_D <3.0 nM.
• Source: E. coli-derived mouse Neurturin protein
  Pro96-Val195
• Accession #: P97463.1
• N-terminal Sequence: Pro96
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Nrtn
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
  • Bioactivity
• Theoretical MW: 11.7 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Neurturin Protein

• Neurturin
• NRTN
• NTN

Background

Neurturin is a member of the GDNF family of ligands, which include glial cell-derived neurotrophic factor (GDNF), Neurturin, Persephin, and Artemin. GDNF family proteins are distant members of the Transforming Growth Factor beta (TGF-beta ) superfamily (1-4). Similar to other TGF-beta family proteins, Neurturin is synthesized as a precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Neurturin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Biologically active Neurturin is a disulfide-linked homodimer of the carboxy-terminal 100 amino acid residues. Mature mouse Neurturin shares 91% amino acid sequence similarity with human Neurturin. Mature Neurturin also shares about 40% similarities with the other three members of the GDNF family ligands (2, 5). Unlike other members of TGF-beta family, bioactivities of all GDNF family ligands are mediated through a unique multicomponent receptor complex composed of high affinity ligand binding component (GFR alpha -1-GFR alpha -4) and a common signaling component (cRET receptor tyrosine kinase). Each member of the GDNF family ligands has its preferred binding protein. Neurturin preferentially binds to GFR alpha -2 but can also bind GFR alpha -1 at higher concentration (5-8). Neurturin has been shown to promote the survival of a variety of neurons including sympathetic, sensory, and central nervous system neurons. Neurturin is expressed in both neuronal and non-neuronal tissues. It may play a role in regulating the development and maintenance of the central and peripheral nervous systems as well as non-neuronal systems (9).

1. Lin, L-FH. et al. (1993) Science 260:1130.
2. Kotzbauer, P.T. et al. (1996) Nature 384:467.
3. Milbrandt, J. et al. (1998) Neuron 20:245.
4. Baloh, R.H. et al. (1998) Neuron 21:1291.
5. Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361.
6. Baloh, R.H. et al. (1997) Neuron 18:793.
7. Jing, S. et al. (1996) Cell 85:1113.
8. Jing, S. et al. (1997) J. Biol. Chem. 272:33111.
9. Airaksinen, M. and M. Saarma (2002) Nature Review Neuroscience 3:383.

Publications for Neurturin (477-MN)(7)

Publications using 477-MN

• JR Cabrera, R Manivanh, BJ North, DA Leib The ESCRT-Related ATPase Vps4 Is Modulated by Interferon during Herpes Simplex Virus 1 Infection MBio, 2019-03-05;10(2):. 2019-03-05 [PMID: 30837340] (Cell Culture, Mouse)
• S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons Proc. Natl. Acad. Sci. U.S.A, 2017-03-07;114(12):E2486-E2493. 2017-03-07 [PMID: 28270618] (Bioassay, Mouse)
• Rosato P, Leib D Neuronal Interferon Signaling Is Required for Protection against Herpes Simplex Virus Replication and Pathogenesis. PLoS Pathog, 2015-07-08;11(7):e1005028. 2015-07-08 [PMID: 26153886] (Bioassay, Mouse)
• Rosato P, Leib D Intrinsic innate immunity fails to control herpes simplex virus and vesicular stomatitis virus replication in sensory neurons and fibroblasts. J Virol, 2014-06-18;88(17):9991-10001. 2014-06-18 [PMID: 24942587] (Bioassay, Mouse)
• Knox , Sarah M, Lombaert , Isabelle, Haddox , Candace, Abrams , Shaun R, Cotrim , Ana, Wilson , Adrian J, Hoffman , Matthew Parasympathetic stimulation improves epithelial organ regeneration. Nat Commun, 2013-01-01;4(0):1494. 2013-01-01 [PMID: 23422662] (Bioassay, Mouse)
• Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo. J. Neurosci., 2006-08-16;26(33):8588-99. 2006-08-16 [PMID: 16914685] (In Vivo, Mouse)
• Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM Expression and function of GDNF family ligands and receptors in the carotid body. Exp. Neurol., 2005-02-01;191(0):S68-79. 2005-02-01 [PMID: 15629763] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: Nrtn
• Uniprot:
  • P97463.1()