Recombinant Mouse Neuroglycan C/CSPG5 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Neuroglycan C/CSPG5 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit Recombinant Human Midkine (Catalog # 258-MD) induced neurite outgrowth in E16-E18 rat embryonic cortical neurons. When 5 x 10⁴ neurons per well are added to a plate pre-incubated with serial dilutions of rmNeuroglycan-C and 1 μg/mL of rhMidkine, the neurite outgrowth promotion is significantly inhibited in a dose dependent manner with an effect at
  0.6-1.2 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Neuroglycan C/CSPG5 protein
  Val31-Gln420, with a C-terminal 6-His tag
• Accession #: AAH55736
• N-terminal Sequence: Val31
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cspg5
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 42.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-105 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Neuroglycan C/CSPG5 Protein, CF

• CALEB
• chondroitin sulfate proteoglycan 5 (neuroglycan C)
• chondroitin sulfate proteoglycan 5
• CSPG5
• MGC44034
• Neuroglycan C
• NGC
• NGCAcidic leucine-rich EGF-like domain-containing brain protein

Background

Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120 - 150 kDa type I transmembrane glycoprotein and member of the neuregulin family of proteins (1 - 2). Depending on its expression profile, NGC may be a glycoprotein of 120 kDa, or a chondroitin sulfate (CS) proteoglycan of 150 kDa (2 - 3). Mouse NGC is synthesized as a 566 amino acid (aa) precursor that contains a 30 aa signal sequence, a 393 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 122 aa cytoplasmic region. The ECD contains one CS attachment domain (aa 32 - 273), with CS attachment at Ser117, one EGF-like domain (aa 371 - 413), three potential sites for N-linked glycosylation, and ten potential sites for O-linked glycosylation (4). Splicing variants produce four isoforms for human NGC. Isoform 1 is the standard form. Isoform 2 has a deletion of aa 487 - 513, while isoform 3 has an alternative start site at Met82 and the same deletion. Isoform 4 has a 56 aa substitution for aa 514 - 566. Phosphorylation likely occurs at Ser249, and proteolysis generates a 75 kDa soluble fragment (5). Over aa 31 - 420, mouse NGC shares 84% aa identity with human NGC. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons (6). NGC may function as a growth and differentiation factor involved in neuritogenesis. One study shows that the recombinant ectodomain of NGC core protein enhances neurite outgrowth from rat neocortical neurons in culture via phosphatidylinositol 3-kinase and protein kinase C signaling pathways (7). Another study states that NGC is a novel component of midkine receptors, a heparin-binding growth factor that promotes cell attachment and process extension in oligodendroglial precursor-like cells (3). NGC also acts as a growth factor by directly binding ERbB3 tyrosine kinase and transactivating ErbB2 (1).

1. Kinugasa, Y. et al. (2004) Biochem. Biophys Res. Commun 321:1045.
2. Yasuda, Y. et al. (1998) Neurosci. Res. 32:313.
3. Ichihara-Tanaka, K. et al. (2006) J. Biol. Chem. 281:30857.
4. Aono, S. et al. (2004) J. Biol. Chem. 279:46536.
5. Shuo, T. et al. (2007) J. Neurochem. 102:1561.
6. Aono, S. et al. (2006) J. Neurosci. Res.83:110.
7. Nakanishi, K., et al. (2006) J. Biol. Chem. 281:24970.

Publications for Neuroglycan C/CSPG5 (5665-NG)(1)

Publication using 5665-NG

• Ng , Aubree A, Logan , Anne M, Schmidt , Eric J, Robinson , Fred L The CMT4B disease-causing phosphatases Mtmr2 and Mtmr13 localize to the Schwann cell cytoplasm and endomembrane compartments, where they depend upon each other to achieve wild-type levels of protein expression. Hum Mol Genet, 2013-01-07;22(8):1493-506. 2013-01-07 [PMID: 23297362] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Cspg5
• Uniprot:
  • AAH55736(Human)