Recombinant Mouse Meteorin Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse Meteorin Protein Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. This protein is able to significantly enhance neurite outgrowth in the range of 60 ng/3 μL drop‑300 ng/3 μL drop.
• Source: Mouse myeloma cell line, NS0-derived mouse Meteorin protein
  Gly22-Asp291 with a C-terminal 6-His tag
• Accession #: Q8C1Q4
• N-terminal Sequence: Gly22
• Protein/Peptide Type: Recombinant Proteins
• Gene: Metrn
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 33 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Meteorin Protein

• C16orf23
• c380A1.2
• Meteorin
• METRN

Background

Meteorin, named after meteor because this protein can transform glial cells into cells with an elongated tail, is a 30 kDa member of the Meteorin family (1). The secreted protein is also known as glial cell differentiation factor and hypoxia/reoxygenation regulatory factor. Mouse Meteorin is synthesized as a 291 amino acid (aa) precursor with a 21 aa signal sequence and a 270 aa mature chain (SwissProt # Q8C1Q4).  Splicing variants produce two isoforms. Isoform 2 is missing residues 1‑164 found in isomer 1, and has a two aa substitution for aa 165-166. Mouse Meteorin is 81% aa identical to human Meteorin (2). Meteorin is expressed in the central nervous system both during development and in adult mice (2). During development, Meteorin mRNA appears both in the central and peripheral nervous systems with the most prominent levels in neural progenitors, glial progenitors, and cells of the astrocyte lineage. In the adult mouse, Meteorin is detected only in the brain (2). Within the brain, the most prominent expression is found in the cerebellum where it is expressed by glial cells interspersed between Purkinje neurons (2). Meteorin is also detectable in several discrete neuronal populations, such as the superior colliculus, the ocular motor nucleus, the raphe and pontine nuclei, and various thalamic nuclei (2).Functionally, Meteorin plays important roles in both glial cell differentiation and axonal network formation during neurogenesis (1). In addition, research shows that when Meteorin is expressed and secreted by perivascular astrocytes, it up-regulates thrombospondin-1/-2 to attenuate angiogenesis in the surrounding endothelial cells and to promote vascular maturation (3).

1. Nishino, J. et al. (2004) EMBO J. 23:1998.
2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. March 4 Published online.
3. Park, J.A. et al. (2008) Glia 56:247.

Publications for Meteorin (3475-MN)(1)

Publication using 3475-MN

• YW Dai, JK Ma, R Jiang, XL Zhan, SY Chen, LL Feng, Q Zhang, TB Liang, K Lv, GJ Yang, JF Lu, J Chen, XJ Lu Meteorin links the bone marrow hypoxic state to hematopoietic stem/progenitor cell mobilization Cell Reports, 2022-09-20;40(12):111361. 2022-09-20 [PMID: 36130501] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Metrn
• Uniprot:
  • Q8C1Q4(Mouse)