Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF Summary

• Additional Information: C-Terminal
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Mesothelin C-Terminal (aa 298-600) His-tag (Catalog 8604-MS) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CA125/MUC16 (Catalog # 5609-MU) that produces 50% optimal binding response is approximately 0.4-2.4 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Mesothelin protein
  Asp298-Ser600, with a C-terminal 6-His tag
• Accession #: Q61468
• N-terminal Sequence: Asp298
• Protein/Peptide Type: Recombinant Proteins
• Gene: Msln
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 38-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Mesothelin (aa 298-600) His Protein, CF

• CAK1 antigen
• CAK1
• megakaryocyte potentiating factor
• Mesothelin
• MPF
• MPFSMRP
• MSLN
• Pre-pro-megakaryocyte-potentiating factor
• SMR
• soluble MPF mesothelin related protein

Background

Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF) (1-3). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF (3, 4). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation (2, 5). The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas (1, 6). It is up-regulated in mesotheliomas and a range of carcinomas and adenomas (7-10). Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression, in part through the up-regulation of MMP-7 (9, 11, 12). It is co-expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion (13). A soluble form of Mesothelin, with its GPI anchor intact, is released from tumor cells and binds to MMR/CD206 on macrophages (10, 14-16).

1. Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
2. Kojima, T. et al. (1995) J. Biol. Chem. 270:21984.
3. Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
4. Onda, M. et al. (2006) Clin. Cancer Res. 12:4225.
5. Yamaguchi, N. et al. (1994) J. Biol. Chem. 269:805.
6. Hou, L.-Q. et al. (2008) Develop. Growth Differ. 50:531.
7. Ordonez, N.G. (2003) Mod. Pathol. 16:192.
8. Argani, P. et al. (2001) Clin. Cancer Res. 7:3862.
9. Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
10. Scholler, N. et al. (1999) Proc. Natl. Acad. Sci. 96:11531.
11. Uehara, N. et al. (2008) Mol. Cancer Res. 6:186.
12. Chang, M.-C. et al. (2012) Biochem. J. 442:293.
13. Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
14. Ho, M. and M.O. Lively (2006) Cancer Epidemiol. Biomarkers Prev. 15:1751.
15. Robinson, B.W.S. et al. (2003) Lancet 362:1612.
16. Dangaj, D. et al. (2011) PLoS ONE 6:e28386.

Bioinformatics

• Gene Symbol: Msln
• Uniprot:
  • Q61468()