Recombinant Mouse MAGP-2/MFAP5 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse MAGP-2/MFAP5 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of BUD‑8 human fibroblast cells.

  When 2 x 10⁴ cells/well are added to Recombinant Mouse MAGP-2/MFAP5 coated plates (10 μg/mL with 100 μL/well), approximately 45-70% will adhere after 1 hour at 37 °C.

  Optimal dilutions should be determinded by each laboratory for each application.

• Source: Chinese Hamster Ovary cell line, CHO-derived mouse MAGP-2/MFAP5 protein
  Gln29-Leu164 with a C-terminal 6-His tag
• Accession #: AAH25131
• N-terminal Sequence: Gln29 predicted, sequencing might be blocked, no result obtained
• Protein/Peptide Type: Recombinant Proteins
• Gene: Mfap5
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 16.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 22-30 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse MAGP-2/MFAP5 Protein, CF

• MAGP2
• MAGP-2
• MAGP2MFAP-5
• MFAP5
• Microfibril-associated glycoprotein 2
• microfibrillar associated protein 5
• microfibrillar-associated protein 5
• MP25
• MP25microfibril-associated glycoprotein-2

Background

MAGP-2 (Microfibril-associated glycoprotein 2), also called MFAP5 (microfibrillar associated protein 5) is a secreted, cell-associated 25 kDa mammalian member of the MFAP family of proteins (1). Mouse MAGP-2 cDNA encodes 164 amino acids (aa) including a 28 aa signal sequence, an RDG motif (aa 30 ‑ 32; absent in MAGP-1) and a central cysteine‑rich binding domain (aa 76 ‑ 133) (1 ‑ 3). Mature mouse MAGP-2 shares 86%, 96%, 84%, 85% and 85% aa identity with human, rat, bovine, porcine and canine MAGP-2, respectively. Both MAGP-1 and MAGP-2 bind and covalently crosslink Fibrillin-1 and -2 through the Cys-rich binding domain. MAGP-2, however, has a more limited distribution and binds a more limited set of extracellular matrix proteins (1 ‑ 3). This may suggest a specialized role in microfibril biology. Functionally, the MAGP-2 RGD motif binds to the integrin alpha v beta 3, allowing cell attachment to microfibrils (4). MAGP‑2 is thought to facilitate microfibril assembly and induce elastin fiber formation (1 ‑ 3, 5). Through its binding to EGF repeats, MAGP‑2 mediates the metalloproteinase-dependent release of Jagged1, and the metalloproteinase-independent release of Notch extracellular domains (6, 7). In endothelial cells, MAGP-2 promotes angiogenic sprouting by inhibiting Notch signaling, by binding to integrin  alpha v beta 3 (which then enhances VEGF signaling), and/or enhancing the endothelial cell response to FGF basic and EGF (8, 9). In ovarian cancer, MAGP-2 expression is associated with cancer cell survival, increased microvessel density, and poor prognosis (10). Skin MAGP-2 expression is increased in human scleroderma and the tight skin (TSK) mouse and may contribute to abnormal collagen accumulation in these conditions by increasing the half-life of type I collagen (11, 12). Mutations within the MAGP-2-binding region of human Fibrillin-1 can be found in Marfan syndrome (5).

1. Gibson, M.A. et al. (1996) J. Biol. Chem. 271:1096.
2. Hanssen, E. et al. (2004) J. Biol. Chem. 279:29185.
3. Segade, F. et al. (2002) J. Biol. Chem. 277:11050.
4. Gibson, M.A. et al. (1999) J. Biol. Chem. 274:13060.
5. Lemaire, R. et al. (2007) J. Biol. Chem. 282:800.
6. Nehring, L.C. et al. (2005) J. Biol. Chem. 280:20349.
7. Miyamoto, A. et al. (2006) J. Biol. Chem. 281:10089.
8. Albig, A.R. et al. (2008) Microvasc. Res. 76:7.
9. Albig, A.R. et al. (2007) Angiogenesis 10:197.
10. Mok, S.C. et al. (2009) Cancer Cell 16:521.
11. Lemaire, R. et al. (2005) Arthritis Rheum. 52:1812.
12. Ito, S. et al. (2006) Am.J. Respir. Cell Mol. Biol. 34:688.

Bioinformatics

• Gene Symbol: Mfap5
• Uniprot:
  • AAH25131()