Recombinant Mouse LSECtin/CLEC4G Protein, CF

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2 μg/lane of Recombinant Mouse LSECtin/CLEC4G (Catalog # 10363-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ...read more
When Recombinant Mouse BTNL4 Fc Chimera (Catalog # 9590-BT) is immobilized at 2 μg/mL (100 μg/well), Recombinant Mouse LSECtin/CLEC4G (Catalog # 10363-CL) binds with an ED50 of 0.15-1.2 μg/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse LSECtin/CLEC4G Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Mouse BTNL4 Fc Chimera (Catalog # 9590-BT) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse LSECtin/CLEC4G (Catalog # 10363-CL) binds with an ED50 of 0.15-1.2 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse LSECtin/CLEC4G protein

Hemagglutinin Tag
(YPYDVPDYA)

Mouse LSECtin/CLEC4G
(Leu52-Tyr294)
Accession # Q8BNX1
N-terminusC-terminus
Accession #
N-terminal Sequence
Tyr
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
36-44 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse LSECtin/CLEC4G Protein, CF

  • CLEC4G
  • C-type lectin domain family 4 member G
  • C-type lectin domain family 4, member G
  • C-type lectin superfamily 4, member G
  • DTTR431
  • liver and lymph node sinusoidal endothelial cell C-type lectin
  • LP2698
  • LSECtin
  • Q6UXB4
  • UNQ431

Background

LSECtin (liver and lymph node sinusoidal endothelial cell C-type lectin), also known as C-type lectin superfamily 4 member G (CLEC4G), is a member of subgroup II of the C-type (Ca2+-dependent) lectin superfamily (1). The protein was named LSECtin because its initial expression was described to be restricted to liver and lymph node sinusoidal endothelial cells (1). However, LSECtin has also been detected in peripheral blood and thymic dendritic cells isolated ex vivo, and in monocyte-derived macrophages and dendritic cells at the RNA and protein level (2). Mouse LSECtin is a type II transmembrane glycoprotein that includes an N-terminal cytoplasmic tail (aa 1-30), a 21 aa transmembrane region, and a 243 aa extracellular domain (ECD). Within the ECD, mouse LSECtin shares 66% and 85% aa sequence identity with human and rat LSECtin, respectively. LSECtin binds to mannose, GlcNAc, and fucose in a Ca2+-dependent manner (1-3). In addition, LSECtin has the ability to bind to surface glycoproteins of enveloped viruses (3, 4). In particular, interaction of LSECtin with the surface glycoproteins of severe acute respiratory syndrome (SARS) coronavirus and Ebola virus has been described, and LSECtin-mediated infection of cells by Ebola virus has been demonstrated (3, 4). LSECtin is highly expressed on tumor-associated macrophages (TAMs) and enhances stemness of breast cancer cells (BCCs) (9). We identified BTN3A3, a B7 family member with previously unknown functions as the receptor for LSECtin on BCCs responsible for stemness-promoting effect of LSECtin (9). In mice bearing human tumor xenografts, either macrophage-specific ablation of LSECtin or silencing of BTN3A3 in BCCs decreased CSC frequency and tumor growth (9). Administration of LSECtin-positive macrophages increased the tumorigenic activity of BCCs dependent on BTN3A3 (9). Disruption of the LSECtin-BTN3A3 axis with BTN3A3-Fc or anti-BTN3A3 mAb has a therapeutic effect on breast cancer (9). These findings define a juxtacrine signaling mechanism by which TAMs promote cancer stemness (9). Targeting this axis in the CSC niche may provide potential therapies to breast cancer (9).
  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Dominguez-Soto, A. et al. (2007) Blood 109:5337.
  3. Powlesland, A. et al. (2008) J. Biol. Chem. 283:593.
  4. Gramberg, T. et al. (2005) Virology 340:224.
  5. Yamashiro, H. et al. (2010) J. Leukoc Biol. 88:757.
  6. Compte, E. et al. (2004) Eur. J. Immunol. 34:2089.
  7. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  8. Bas, A. et al. (2011) Proc Natl Acad Sci U S A. 108:4376.
  9. Liu, D. et al. (2019) Cell Res. 29:5.

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