Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein, CF Summary
Details of Functionality |
Measured by its binding ability in a functional ELISA. In a Goat Anti-Mouse IgG Fc Antibody
(Catalog #
G-202-C)
coated plate, Recombinant Mouse LRP‑6 N-Terminal Fragment Fc Chimera binds Recombinant Mouse Dkk-1
(Catalog #
5897-DK)
with an ED 50 of 15-120 ng/mL. |
Source |
Chinese Hamster Ovary cell line, CHO-derived mouse LRP-6 protein Mouse LRP-6 (Ala20-Ile628) Accession # NP_032540 | IEGRMDP | Mouse IgG2a (Glu98-Lys330) | N-terminus | | C-terminus | |
|
Accession # |
|
N-terminal Sequence |
Ala20 |
Structure / Form |
Disulfide-linked homodimer
|
Protein/Peptide Type |
Recombinant Proteins |
Purity |
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Theoretical MW |
96 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
100-120 kDa, reducing conditions |
Packaging, Storage & Formulations
Storage |
- 12 months from date of receipt, ≤ -20 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, ≤ -20 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity |
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions |
Reconstitute at 500 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse LRP-6 N-Terminal Fragment Fc Protein, CF
Background
The low-density lipoprotein (LDL) receptor-related protein 6 (LRP-6), along with LRP-5, constitute a distinct subgroup of the LDL receptor family (1). Both LRP-5 and LRP-6 are type I transmembrane proteins that function as Wnt co-receptors with Frizzled proteins and mediate the down-regulation of GSK-3 activity and the initiation of the canonical Wnt/beta-catenin signaling cascade (1, 2). Mouse LRP-6 consists of a 19 amino acid (aa) signal sequence, a 1351 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 20 aa cytoplasmic domain (3). The LRP-6 ECD is composed of four distinct YWTD beta -propeller (BP) motifs followed by three LDLR type A repeats (4). The first two LRP-6 BP motifs bind Wnt-1, -2, -2b, -6, -8a, -9a, -9b,
and -10b, while the third and fourth BP motifs bind Wnt-3a (5-10). This product includes the first and second BP motifs of mouse LRP-6 (aa 20-628) that share 98% aa sequence identity with the first two BP motifs of rat and human LRP-6. Dkk-1, a known antagonist of Wnt signaling, interacts with LRP-6 as a bipartite inhibitor, binding the first and third BP motifs of LRP-6 simultaneously (8, 11). Mice lacking LRP-6 die at birth with a truncation of the axial skeleton, limb defects, microphthalmia, and malformation of the urogenital system (2). Additionally, LRP-6 may be essential for osteoblast differentiation during bone remodeling and, along with LRP-5, is required for postnatal bone development (12, 13).
- Joiner, D.M. et al. (2013) Trends Endocrinol. Metab. 24:31.
- Pinson, K.I. et al. (2000) Nature 407:535.
- Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248:879.
- He, X. et al. (2004) Development 131:1663.
- Ai, M. et al. (2005) Mol. Cell. Biol. 25:4946.
- Gong, Y. et al. (2010) PLoS One 5:e12682.
- Itasaki, N. et al. (2003) Development 130:4295.
- Mao, B. et al. (2001) Nature 411:321.
- Zhang, Y. et al. (2004) Mol. Cell. Biol. 24:4677.
- Bourhis, E. et al. (2010) J. Biol. Chem. 285:9172.
- Ahn, V.E. et al. (2011) Dev. Cell 21:862.
- Li, C. et al. (2013) J. Bone Miner. Res. 28:2094.
- Riddle, R.C. et al. (2013) PLoS One 8:e63323.
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