Recombinant Mouse Integrin alpha V beta 5 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Integrin alpha V beta 5 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Vitronectin (Catalog # 2308-VN) is coated at 1 μg/mL, Recombinant Mouse Integrin alpha V beta 5 binds with an apparent K_D <0.5 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha V beta 5 protein
  

  Mouse Integrin alpha V (Phe31-Val988) Accession # P43406		HP	GS Linker	Acidic Tail	HHHHHH
  Mouse Integrin beta 5 (Gly24-Asn719) Accession # NP_001139356		HP	GS Linker	Basic Tail
  N-terminus					C-terminus

• Accession #: P43406 (Integrin alpha V) & NP_001139356 (Integrin beta 5)
• N-terminal Sequence: Phe31 ( alpha V subunit) & Gly24 ( beta 5 subunit)
• Structure / Form: Noncovalently-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 115 kDa ( alpha V subunit) & 84.8 kDa ( beta 5 subunit).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 140-155 kDa & 105-115 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Integrin alpha V beta 5 Protein, CF

• CD51
• Integrin alpha V beta 5
• integrin subunit alpha V
• ITGAV
• MSK8
• VNRA
• VTNR

Background

Integrin  alpha V beta 5 is one of five alpha V integrins and the only known beta 5 integrin (1‑3). The non‑covalent heterodimer of 170 kDa alpha V and 100‑110 kDa beta 5 integrin type I transmembrane glycoprotein subunits is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium (4‑8). alpha V beta 5 binds ligands containing an RGD motif, notably vitronectin (1‑10). The 958 aa mouse alpha V extracellular domain (ECD) shares 92‑95% aa sequence identity with human and bovine alpha V, while the 696 aa mouse beta 5 ECD shares 97%, 91% and 91% aa sequence identity with rat, human and bovine beta 5, respectively. The alpha V ECD contains an N‑terminal beta ‑propeller structure, followed by domains termed thigh, calf‑1 and calf‑2 (1). The 799 aa beta 5 contains a vWFA domain within the ECD, which interacts with the alpha V beta ‑propeller to form a binding domain. Each subunit has a transmembrane sequence and a short cytoplasmic tail. Potential beta 5 isoforms include a 691 aa form with an alternate start site at aa 109, a 958 aa form with an alternate N‑terminus, and a 795 aa form with an alternate C‑terminus. Post‑translational modifications, such as proteolytic cleavage of the alpha V subunit or phosphorylation of the beta 5 cytoplasmic tail, can increase endocytic turnover of the alpha V beta 5 protein and/or promote cell migration (7‑10). Growth factors that increase PKC activity, such as VEGF or TGF‑ alpha , promote alpha V beta 5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF‑basic and TNF‑ alpha (11). An inhibitor of both down‑regulates tumor angiogenesis (12). During lung inflammation, up‑regulation of alpha V beta 5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF‑ beta from latency (13, 14). On retinal pigment epithelia, alpha V beta 5 is important for normal diurnal phagocytosis of outer rod segments, and contributes to adhesion of retinal cells (15).

1. Hynes, R.O. (2002) Cell 110:673.
2. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14080.
3. Suzuki, S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5354.
4. Smith, J.W. et al. (1990) J. Biol. Chem. 265:11008.
5. Pasqualini, R. et al. (1993) J. Cell Sci. 105:101.
6. Braam, S.R. et al. (2008) Stem Cells 26:2257.
7. Memmo, L.M. and P. McKeown-Longo (1998) J. Cell Sci. 111:425.
8. Kim, J.P. et al. (1994) J. Biol. Chem. 269:26926.
9. Berthet, V. et al. (2004) FEBS Lett. 557:159.
10. Li, Z. et al. (2010) Mol. Biol. Cell 21:3317.
11. Friedlander, M. et al. (1995) Science 270:1500.
12. Desgrosellier, J.S. and D.A. Cheresh (2010) Nat. Rev. Cancer 10:9.
13. Wipff, P.J. et al. (2007) J. Cell Biol. 179:1311.
14. Luzina, I.G. et al. (2009) Arthritis Rheum. 60:1530.
15. Nandrot, E.F. et al. (2004) J. Exp. Med. 200:1539.

Publications for Integrin alpha V beta 5 (7706-AV)(1)

Publication using 7706-AV

• M Bieri, R Hendrickx, M Bauer, B Yu, T Jetzer, B Dreier, PRE Mittl, J Sobek, A Plückthun, UF Greber, S Hemmi The RGD-binding integrins alphavbeta6 and alphavbeta8 are receptors for mouse adenovirus-1 and -3 infection PloS Pathogens, 2021-12-15;17(12):e1010083. 2021-12-15 [PMID: 34910784] (Bioassay, Mouse)

Bioinformatics

• Uniprot:
  • P43406 (Integrin alpha V) & NP_001139356 (Integrin beta 5)()