Recombinant Mouse Integrin alpha 6 beta 1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Integrin alpha 6 beta 1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Laminin alpha 4 (Catalog # 7340-A4) is coated at 5 μg/mL, Recombinant Mouse Integrin alpha 6 beta 1 binds with an apparent K_D <5nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 6 beta 1 protein
  

  Mouse Integrin alpha 6 (Phe24-Gly1011) Accession # Q61739	HP	GGGSGGGS	Acidic Tail	HHHHHH
  Mouse Integrin beta 1 (Gln21-Asp728) Accession # P09055	HP	GGGSGGGS	Basic Tail
  N-terminus			C-terminus

• Accession #: Q61739 ( alpha 6 subunit) & P09055 ( beta 1 subunit)
• N-terminal Sequence: Phe24 ( alpha 6 subunit) & Gln21 predicted: No results obtained, sequencing might be blocked ( beta 1 subunit)
• Structure / Form: Noncovalently-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 119 kDa ( alpha 6 subunit) & 86.4 kDa ( beta 1 subunit).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 105-120 & 125-150 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Integrin alpha 6 beta 1 Protein, CF

• CD49f
• Integrin alpha 6 beta 1
• ITGA6B
• VLA-6

Background

Integrin alpha 6 beta 1, also called platelet glycoprotein GPIc-IIa, is a laminin binding integrin that is expressed on T cells, monocytes, endothelial cells, stem cells, and platelets (1-9). The non-covalent heterodimer is composed of ~150 kDa alpha 6/CD49f and 130 kDa beta 1/CD29 type I transmembrane glycoprotein subunits (2). While alpha 6 pairs only with beta 1 or beta 4, twelve integrins share the beta 1 subunit (1‑5). The alpha 6 subunit is cleaved into extracellular heavy and transmembrane light chains (3). Alternative splicing in the human alpha 6 extracellular domain (ECD) at amino acid (aa) 216 creates X1 (ubiquitous), X2 and X1X2 isoforms, while splicing at a mouse or human cytoplasmic site creates A and B isoforms (10, 11). These forms do not appear to alter the binding specificity (4, 10, 11). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside‑out) signaling convert it to its most active, extended and open conformation (1, 2). The mouse alpha 6 heavy chain shares 98% aa identity with rat and 92‑93% with human (X1), bovine, and canine  alpha 6, and the mouse beta 1 ECD shares 98% aa identity with rat and 93‑94% with human, bovine, porcine, ovine, canine and feline beta 1. alpha 6 beta 1 shows broad specificity for adhesion to laminin isoforms (4, 10). Its expression on human and mouse pluripotent stem cells is important for attachment, expansion, and self-renewal on LN‑511 (laminin alpha ₅ beta ₁ gamma ₁) (6, 7). The secreted protein Netrin‑4 and the laminin gamma ₁ subunit form an adhesion‑activating complex with alpha 6 beta 1 on mouse neural stem cells and human lymphatic endothelial cells that promotes lymphangiogenesis (8, 9). alpha 6 beta 1 up‑regulation on cancers such as prostate, glioma, and hepatoma is reported to enhance tumorigenicity, motility, invasion and metastasis (12‑14). alpha 6 beta 1 cleavage via uPA (urokinase‑type plasminogen activator) facilitates tumorigenicity in prostate cancers, and interaction of hepatoma alpha 6 beta 1 with EMMPRIN/CD147 may also enhance tumorigenicity by inducing uPA and other metalloproteinases (12, 13).

1. Takada, Y. et al. (2007) Genome Biol. 8:215.
2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
3. Tamura, R.N. et al. (1990) J. Cell Biol. 111:1593.
4. Nishiuchi, R. et al. (2006) Matrix Biol. 25:189.
5. Sonnenberg, A. and C.J.T. Linders (1990) J. Cell Science 96:207.
6. Rodin, S. et al. (2010) Nat. Biotech. 28:611.
7. Domogatskaya A. et al. (2008) Stem Cells 26:2800.
8. Staquicini, F.I. et al. (2009) Proc. Natl. Acad. Sci. USA 106:2903.
9. Larrieu-Lahargue, F. et al. (2011) Circ. Res. 109:770.
10. Delwel, G. O. et al. (1995) Cell Adhes. Commun. 3:143.
11. Hogervorst, F. et al. (1993) J. Cell Biol. 121:179.
12. Sroka, I.C. et al. (2011) Mol. Cancer Res. 9:1319.
13. Dai, J.Y. et al. (2009) BMC Cancer 9:337.
14. Delamarre, E. et al. (2009) Am. J. Pathol. 175:844.

Bioinformatics

• Uniprot:
  • Q61739 ( alpha 6 subunit) & P09055 ( beta 1 subunit)()