Recombinant Mouse IL-2 Protein

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1 μg/lane of Recombinant Mouse IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band(s) at 19 kDa.
Recombinant Mouse IL-2 (Catalog # 402‑ML) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.1‑0.4 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.1-0.4 ng/mL.
Source
E. coli-derived mouse IL-2 protein
Ala21-Gln169, with and without an N-terminal Met
Accession #
N-terminal Sequence
Ala21 & Met
Protein/Peptide Type
Recombinant Proteins
Gene
Il2
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
402-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and Tween®-80 with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-2 Protein

  • aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

Interleukin‑2 (IL‑2) is a O‑glycosylated four alpha ‑helix bundle cytokine that has potent stimulatory activity for antigen‑activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1‑3). Mature mouse IL‑2 shares 56% and 73% aa sequence identity with human and rat IL‑2, respectively. It shows strain‑specific heterogeneity in an N‑terminal region that contains a poly‑glutamine stretch (4). Mouse and human IL‑2 exhibit cross‑species activity (5). The receptor for IL‑2 consists of three subunits that are present on the cell surface in varying preformed complexes (6‑8). The 55 kDa IL‑2 R alpha is specific for IL‑2 and binds with low affinity. The 75 kDa IL‑2 R beta, which is also a component of the IL‑15 receptor, binds IL‑2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL‑4, ‑7, ‑9, ‑15, and ‑21, does not independently interact with IL‑2. Upon ligand binding, signal transduction is performed by both IL‑2 R beta and gamma c. IL‑2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL‑2 and IL‑2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas‑induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (9). IL‑2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (10‑12). Thus, IL‑2 may be a key cytokine in the natural suppression of autoimmunity (13, 14).

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Kashima, N. et al. (1985) Nature 313:402.
  4. Matesanz, F. et al. (1993) Immunogenetics 38:300.
  5. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  6. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  7. Wang, X. et al. (2005) Science 310:1159.
  8. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  9. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  10. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  11. Laurence, A. et al. (2007) Immunity 26:371.
  12. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  13. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  14. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

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Publications for IL-2 (402-ML)(143)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 3 applications: Bioassay, In Vivo, cell culture.


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Bioassay
(133)
In Vivo
(6)
cell culture
(1)
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(1)
Mouse
(139)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
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Showing Publications 1 - 10 of 143. Show All 143 Publications.
Publications using 402-ML Applications Species
S Liu, M Xu, C Zhu, Q Zhao, F Zhou Taste Receptor T1R1/T1R3 Promotes the Tumoricidal Activity of Hepatic CD49a+CD49b- Natural Killer Cells Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 30259960] (Bioassay, Mouse) Bioassay Mouse
V Jelen?i?, M Šestan, I Kavazovi?, M Lenarti?, S Marinovi?, TD Holmes, M Prchal-Mur, B Lisni?, V Sexl, YT Bryceson, FM Wensveen, B Poli? NK cell receptor NKG2D sets activation threshold for the NCR1 receptor early in NK cell development Nat. Immunol., 2018;0(0):. 2018 [PMID: 30224819] (Bioassay, Mouse) Bioassay Mouse
J Wu, J Gu, S Zhou, H Lu, Y Lu, L Lu, X Wang Anti-IL-22 Antibody Attenuates Acute Graft-versus-Host Disease via Increasing Foxp3+ T Cell through Modulation of CD11b+ Cell Function J Immunol Res, 2018;2018(0):1605341. 2018 [PMID: 30159338] (Bioassay, Mouse) Bioassay Mouse
CD Geary, C Krishna, CM Lau, NM Adams, SV Gearty, Y Pritykin, AR Thomsen, CS Leslie, JC Sun Non-redundant ISGF3 Components Promote NK Cell Survival in an Auto-regulatory Manner during Viral Infection Cell Rep, 2018;24(8):1949-1957.e6. 2018 [PMID: 30134157] (Bioassay, Mouse) Bioassay Mouse
HA Ting, D de Almeida, AJ Rasky, CA Malinczak, IP Maillard, MA Schaller, NW Lukacs Notch ligand Delta-like 4 induces epigenetic regulation of Treg cell differentiation and function in viral infection Mucosal Immunol, 2018;0(0):. 2018 [PMID: 30038214] (Bioassay, Mouse) Bioassay Mouse
X Zhang, X Xiao, P Lan, J Li, Y Dou, W Chen, N Ishii, S Chen, B Xia, K Chen, E Taparowsky, XC Li OX40 Costimulation Inhibits Foxp3 Expression and Treg Induction via BATF3-Dependent and Independent Mechanisms Cell Rep, 2018;24(3):607-618. 2018 [PMID: 30021159] (Bioassay, Mouse) Bioassay Mouse
Y Omata, M Frech, T Primbs, S Lucas, D Andreev, C Scholtysek, K Sarter, M Kindermann, N Yeremenko, DL Baeten, N Andreas, T Kamradt, A Bozec, A Ramming, G Krönke, S Wirtz, G Schett, MM Zaiss Group 2 Innate Lymphoid Cells Attenuate Inflammatory Arthritis and Protect from Bone Destruction in Mice Cell Rep, 2018;24(1):169-180. 2018 [PMID: 29972778] (In Vivo, Mouse) In Vivo Mouse
HH Abdul-Raza, CJ Rocca, SJ Howe, ME Alonso-Fer, J Wang, R Gabriel, CC Bartholoma, CHV Gan, MI Garín, A Roberts, MP Blundell, V Prakash, FJ Molina-Est, J Pantoglou, G Guenechea, MC Holmes, PD Gregory, C Kinnon, C von Kalle, M Schmidt, JA Bueren, AJ Thrasher, RJ Yáñez-Muño Molecular Evidence of Genome Editing in a Mouse Model of Immunodeficiency Sci Rep, 2018;8(1):8214. 2018 [PMID: 29844458] (Bioassay, Mouse) Bioassay Mouse
S Teruya, T Okamura, T Komai, M Inoue, Y Iwasaki, S Sumitomo, H Shoda, K Yamamoto, K Fujio Egr2-independent, Klf1-mediated induction of PD-L1 in CD4+ T cells Sci Rep, 2018;8(1):7021. 2018 [PMID: 29728568] (Bioassay, Mouse) Bioassay Mouse
R Yang, T Yu, D Liu, S Shi, Y Zhou Hydrogen sulfide promotes immunomodulation of gingiva-derived mesenchymal stem cells via the Fas/FasL coupling pathway Stem Cell Res Ther, 2018;9(1):62. 2018 [PMID: 29523215] (Mouse) Mouse
Show All 143 Publications.

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Bioinformatics

Gene Symbol Il2
Uniprot