Recombinant Mouse IL-2 (Cys160Ser) Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse IL-2 (Cys160Ser) Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED₅₀ for this effect is 0.04‑0.24 ng/mL.
• Source: E. coli-derived mouse IL-2 protein
  Ala21-Gln169 (Cys160Ser), with and without an N-terminal Met
• Accession #: P04351.1
• N-terminal Sequence: Ala21 & Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Sodium Acetate with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-2 (Cys160Ser) Protein

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

Interleukin-2 (IL-2) is an O-glycosylated four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4⁺ and CD8⁺ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1 - 3). Mature mouse IL-2 shares 56% and 73% amino acid (aa) sequence identity with human and rat IL-2, respectively. It shows strain-specific heterogeneity in an N-terminal region that contains a poly-glutamine stretch (4). Mouse and human IL-2 exhibit cross-species activity (5). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (6 - 8). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta , which is also a component of the IL‑15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma , which is shared with the receptors for IL-4, -7, -9, -15, and ‑21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL‑2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4⁺ T cells but not activated CD4⁺ memory lymphocytes (9). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (10 - 12). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (13, 14).

1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
3. Kashima, N. et al. (1985) Nature 313:402.
4. Matesanz, F. et al. (1993) Immunogenetics 38:300.
5. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
6. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
7. Wang, X. et al. (2005) Science 310:1159.
8. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
9. Jaleco, S. et al. (2003) J. Immunol. 171:61.
10. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
11. Laurence, A. et al. (2007) Immunity 26:371.
12. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
13. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
14. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

Publications for IL-2 (1150-ML)(1)

Publication using 1150-ML

• Liu X, Leung S, Wang C, Tan Z, Wang J, Guo TB, Fang L, Zhao Y, Wan B, Qin X, Lu L, Li R, Pan H, Song M, Liu A, Hong J, Lu H, Zhang JZ Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease. Nat. Med., 2010-01-10;16(2):191-7. 2010-01-10 [PMID: 20062065] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Il2
• Uniprot:
  • P04351.1()