Recombinant Mouse IL-13 Protein

Images

 
1 μg/lane of Recombinant Mouse IL-13 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 9 kDa.
Recombinant Mouse IL-13 (Catalog # 413‑ML) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.75-3 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Mouse IL-13 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.75‑3 ng/mL.
Source
E. coli-derived mouse IL-13 protein
Ser26-Phe131
Accession #
N-terminal Sequence
Ser26
Protein/Peptide Type
Recombinant Proteins
Gene
Il13
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
11.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
9 kDa, reducing conditions
Publications
Read Publications using
413-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-13 Protein

  • ALRHMGC116789
  • BHR1interleukin-13
  • IL13
  • IL-13
  • IL-13MGC116788
  • interleukin 13
  • MGC116786
  • NC30
  • P600

Background

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1 - 3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11 - 14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

  1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
  2. Nakajima, H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
  3. Brown, K.D. et al. (1989) J. Immunol. 142:679.
  4. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
  5. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
  6. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  7. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
  8. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
  9. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  10. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
  11. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
  12. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
  13. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
  14. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
  15. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  16. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
  17. Andrews, A.-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.

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Publications for IL-13 (413-ML)(57)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 4 applications: Bioassay, ELISA Standard, In Vivo, Luminex standard.


Filter By Application
Bioassay
(35)
ELISA Standard
(6)
In Vivo
(15)
Luminex standard
(1)
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Human
(2)
Mouse
(50)
N/A
(5)
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Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using 413-ML Applications Species
Y Kasagi, PM Chandramou, KA Whelan, K Tanaka, V Giroux, M Sharma, J Wang, AJ Benitez, M DeMarshall, JW Tobias, KE Hamilton, GW Falk, JM Spergel, AJ Klein-Szan, AK Rustgi, AB Muir, H Nakagawa The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial�Changes Cell Mol Gastroenterol Hepatol, 2018;5(3):333-352. 2018 [PMID: 29552622] (Bioassay, Mouse) Bioassay Mouse
S Wan, X Sun, F Wu, Z Yu, L Wang, D Lin, Z Li, Z Wu, X Sun Chi3l3: a potential key orchestrator of eosinophil recruitment in meningitis induced by Angiostrongylus cantonensis J Neuroinflammation, 2018;15(1):31. 2018 [PMID: 29391024] (Bioassay, Mouse) Bioassay Mouse
H Yang, Y Chen, C Gao Interleukin-13 reduces cardiac injury and prevents heart dysfunction in viral myocarditis via enhanced M2 macrophage polarization Oncotarget, 2017;8(59):99495-99503. 2017 [PMID: 29245918] (In Vivo, Mouse) In Vivo Mouse
T Kerrinnes, MG Winter, BM Young, VE Diaz-Ochoa, SE Winter, RM Tsolis Utilization of host polyamines in alternatively activated macrophages promotes chronic infection by Brucella abortus Infect. Immun., 2017;0(0):. 2017 [PMID: 29203548] (Bioassay, Mouse) Bioassay Mouse
R Bruen, S Curley, S Kajani, D Crean, ME O'Reilly, MB Lucitt, CG Godson, FC McGillicud, O Belton Liraglutide dictates macrophage phenotype in apolipoprotein E null mice during early atherosclerosis Cardiovasc Diabetol, 2017;16(1):143. 2017 [PMID: 29110715] (Bioassay, Mouse) Bioassay Mouse
A Nasti, Y Sakai, A Seki, GB Buffa, T Komura, H Mochida, M Yamato, K Yoshida, TTB Ho, M Takamura, S Usui, T Wada, M Honda, S Kaneko The CD45(+) fraction in murine adipose tissue-derived stromal cells harbor immune-inhibitory inflammatory cells Eur. J. Immunol., 2017;0(0):. 2017 [PMID: 28891216] (Bioassay, Mouse) Bioassay Mouse
CA Sorgi, S Zarini, SA Martin, RL Sanchez, RF Scandiuzzi, MA Gijón, C Guijas, N Flamand, RC Murphy, LH Faccioli Dormant 5-lipoxygenase in inflammatory macrophages is triggered by exogenous arachidonic acid Sci Rep, 2017;7(1):10981. 2017 [PMID: 28887514] (Bioassay, Mouse) Bioassay Mouse
AS Morris, SC Sebag, JD Paschke, A Wongrakpan, K Ebeid, ME Anderson, IM Grumbach, AK Salem Cationic CaMKII Inhibiting Nanoparticles Prevent Allergic Asthma Mol. Pharm., 2017;0(0):. 2017 [PMID: 28460526] (Bioassay, Mouse) Bioassay Mouse
T Sezaki, Y Hirata, T Hagiwara, YI Kawamura, T Okamura, R Takanashi, K Nakano, M Tamura-Nak, LC Burkly, T Dohi Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice World J. Gastroenterol., 2017;23(13):2294-2307. 2017 [PMID: 28428709] (Bioassay, Mouse) Bioassay Mouse
DC Nascimento, PH Melo, AR Piñeros, RG Ferreira, DF Colón, PB Donate, FV Castanheir, A Gozzi, PG Czaikoski, W Niedbala, MC Borges, DS Zamboni, FY Liew, FQ Cunha, JC Alves-Filh IL-33 contributes to sepsis-induced long-term immunosuppression by expanding the regulatory T cell population Nat Commun, 2017;8(0):14919. 2017 [PMID: 28374774] (Bioassay, Mouse) Bioassay Mouse
Show All 57 Publications.

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Bioinformatics

Gene Symbol Il13
Uniprot