Recombinant Mouse IL-12 R beta 2 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse IL-12 R beta 2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Mouse (rm) IL‑12 R beta 2 Fc Chimera is immobilized at 0.5 μg/mL (100 μL/well), the concentration of rmIL-12 that produces 50% of the optimal binding response is found to be approximately 4 ‑ 20 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-12 R beta 2 protein
Mouse IL-12 R beta 2
(Met1 - Asn637)
Accession # P97378
IEGRMDP Mouse IgG2A
(Glu98 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Asn24
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il12rb2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
95.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
125-145 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-12 R beta 2 Fc Chimera Protein, CF

  • IL-12 R beta 2
  • IL-12 receptor beta 2
  • IL-12 receptor subunit beta-2
  • IL12R beta 2
  • IL-12R subunit beta-2
  • IL12RB2
  • IL-12Rb2
  • IL-12R-beta-2
  • interleukin 12 receptor, beta 2
  • interleukin-12 receptor beta-2 chain
  • interleukin-12 receptor subunit beta-2

Background

The high‑affinity IL‑12 receptor complex includes the 100 kDa IL‑12 receptor beta 1 (IL‑12 R beta 1) and the 130 kDa IL‑12 Receptor  beta 2 (IL‑12 R beta 2) subunits, both of which are type I transmembrane proteins that belong to the cytokine receptor superfamily (1, 2). The complex's ligand, IL‑12, is a disulfide‑linked heterodimer composed of 35 kDa (IL‑12 alpha  p35) and 40 kDa (IL‑12 beta  p40) subunits. IL‑12 R beta 2 binds IL‑12 alpha and signals through Jak2, while IL‑12 R beta 1 binds IL‑12 beta and signals through Tyk2 (3). IL‑12 R beta 1 is also a subunit of the IL‑23 receptor complex (3). The 874 amino acid (aa) mouse IL‑12 R beta 2 precursor includes a 23 aa signal peptide, a 614 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 216 aa cytoplasmic region. The ECD possesses one C2‑type Ig‑like domain, five fibronectin type III (Fn III) repeats, 14 potential N‑glycosylation sites, and a WSXWS motif, while the cytoplasmic region contains a Box 1 motif and three tyrosine phosphorylation sites that presumably mediate intracellular signaling (3). The mouse IL‑12 R beta 2 ECD shares 91% aa sequence identity with rat IL‑12 R beta 2, and 68% with human, porcine and bovine IL‑12 R beta 2. Human and mouse IL‑12 R beta 2 do not bind cross‑species IL‑12 (2). A 734 aa mouse isoform that lacks aa 363 ‑ 503 within the Fn III domains is reported (4). Unlike IL‑12 R beta 1, which is constitutively expressed on T cells, NK cells and B cells, IL‑12 R beta 2 expression is more restricted (2). On naïve T cells, IL‑12 R beta 2 is expressed following STAT1 activation by IFN‑ gamma, IL‑27 and/or T cell receptor ligation. This up‑regulation allows IL‑12 to promote Th1, but not Th2, differentiation (5 ‑ 7). Among B cells, surface expression is limited to naïve germinal center and memory B cells, and myeloma cells (2). Deletion of IL‑12 R beta 2 causes systemic overexpression of IL‑6, accelerated maturation of thymocytes, deficient regulatory T cell maturation and function, and reduced splenic T cell apoptosis (2, 8 ‑ 10). These mice are susceptible to autoimmune diseases such as experimental autoimmune encephalitis and spontaneous B cell malignancies (2, 8 ‑ 10). In humans, polymorphism of the IL‑12 R beta 2 gene is associated with systemic sclerosis (11).

  1. Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:14002.
  2. Pistoia, V. et al. (2009) J. Clin. Oncol. 27:4809.
  3. Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
  4. GenBank protein Accession # P97378.
  5. Rogge, L. et al. (1997) J. Exp. Med. 185:825.
  6. Becskei, A. and M.J. Grusby (2007) FEBS Lett. 581:5199.
  7. Szabo, S.J. et al. (1997) J. Exp. Med. 185:817.
  8. Zhao, Z. et al. (2008) J. Immunol. 181:3870.
  9. Gran, B. et al. (2010) Exp. Mol. Pathol. 89:126.
  10. Airoldi, I. et al. (2005) Blood 106:3846.
  11. Bossini-Castillo, L. et al. (2011) Hum. Mol. Genet. Nov. 29 [Epub ahead of print].

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Bioinformatics

Gene Symbol Il12rb2
Entrez
Uniprot