Recombinant Mouse IL-1 beta/IL-1F2 Protein


Recombinant Mouse IL-1 beta /IL-1F2 (Catalog # 401-ML) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 2‑10 pg/mL.
1 μg/lane of Recombinant Mouse IL1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.

Product Details

Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations

Recombinant Mouse IL-1 beta/IL-1F2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 2-10 pg/mL.
E. coli-derived mouse IL-1 beta/IL-1F2 protein
Val118-Ser269, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
401-ML in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-1 beta/IL-1F2 Protein

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta


IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65%-78% identity with canine, equine, feline, human, porcine, and rhesus IL-1 beta .

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Gray, P.W. et al. (1986) J. Immunol. 137:3644.
  6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu
Applications: ELISA
Species: Hu
Applications: BA
Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
Species: Hu
Applications: BA
Species: Hu, Mu, Po, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, WB
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ELISA
Species: Mu
Applications: BA
Species: Mu
Applications: Bioactivity

Publications for IL-1 beta/IL-1F2 (401-ML)(263)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bacteria - Porphyromonas gingivalis, Transgenic Mouse.

We have publications tested in 13 applications: Binding Assay, Bioassay, Cell Culture, Degradation, Differentiation, ELISA (Standard), ELISA Standard, IHC, In Vivo, Organ Culture, Tissue Culture, Western Blot, Western Blot Control.

Filter By Application
Binding Assay
Cell Culture
ELISA (Standard)
ELISA Standard
In Vivo
Organ Culture
Tissue Culture
Western Blot
Western Blot Control
All Applications
Filter By Species
Bacteria - Porphyromonas gingivalis
Transgenic Mouse
All Species
Showing Publications 1 - 10 of 263. Show All 263 Publications.
Publications using 401-ML Applications Species
Lilley, RJ;Taylor, KD;Wildman, SSP;Peppiatt-Wildman, CM; Inflammatory mediators act at renal pericytes to elicit contraction of vasa recta and reduce pericyte density along the kidney medullary vascular network Frontiers in physiology 2023-06-09 [PMID: 37362447] (Bioassay, Mouse) Bioassay Mouse
Zhai, R;Lenga Ma Bonda, W;Leclaire, C;Saint-Béat, C;Theilliere, C;Belville, C;Coupet, R;Blondonnet, R;Bouvier, D;Blanchon, L;Sapin, V;Jabaudon, M; Effects of sevoflurane on lung epithelial permeability in experimental models of acute respiratory distress syndrome Journal of translational medicine 2023-06-18 [PMID: 37331963] (Bioassay, Mouse) Bioassay Mouse
Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ; Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration Cell reports 2023-06-16 [PMID: 37330908]
Li, TT;Lin, CL;Chiang, M;He, JT;Hung, CH;Hsieh, CC; Cytokine-Induced Myeloid-Derived Suppressor Cells Demonstrate Their Immunoregulatory Functions to Prolong the Survival of Diabetic Mice Cells 2023-05-29 [PMID: 37296628] (Bioassay, Mouse) Bioassay Mouse
Lu, Y;Zhang, H;Pan, H;Zhang, Z;Zeng, H;Xie, H;Yin, J;Tang, W;Lin, R;Zeng, C;Cai, D; Expression pattern analysis of m6A regulators reveals IGF2BP3 as a key modulator in osteoarthritis synovial macrophages Journal of translational medicine 2023-05-22 [PMID: 37217897] (Bioassay, Mouse) Bioassay Mouse
Wayne, CR;Bremner, L;Faust, TE;Dur�n-Laforet, V;Ampatey, N;Ho, SJ;Feinberg, PA;Arvanitis, P;Ciric, B;Ruan, C;Elyaman, W;Delaney, SL;Vargas, WS;Swedo, S;Menon, V;Schafer, DP;Cutforth, T;Agalliu, D; Distinct Th17 effector cytokines differentially promote microglial and blood-brain barrier inflammatory responses during post-infectious encephalitis bioRxiv : the preprint server for biology 2023-05-09 [PMID: 37215000] (Bioassay, Mouse) Bioassay Mouse
Wan, J;Zhu, Z;He, Z;Wu, H;Chen, A;Zhu, W;Cheng, P; Stevioside protects primary articular chondrocytes against IL-1?-induced inflammation and catabolism by targeting integrin International immunopharmacology 2023-06-01 [PMID: 37167638] (Bioassay, Mouse) Bioassay Mouse
Qu, Y;Wang, Y;Wang, S;Yu, X;He, Y;Lu, R;Chen, S;Meng, C;Xu, H;Pei, W;Ni, B;Zhang, R;Huang, X;You, H; A comprehensive analysis of single-cell RNA transcriptome reveals unique SPP1+ chondrocytes in human osteoarthritis Computers in biology and medicine 2023-06-01 [PMID: 37141654] (Bioassay, Mouse) Bioassay Mouse
Slieker, RC;Donnelly, LA;Akalestou, E;Lopez-Noriega, L;Melhem, R;G�ne?, A;Abou Azar, F;Efanov, A;Georgiadou, E;Muniangi-Muhitu, H;Sheikh, M;Giordano, GN;�kerlund, M;Ahlqvist, E;Ali, A;Banasik, K;Brunak, S;Barovic, M;Bouland, GA;Burdet, F;Canouil, M;Dragan, I;Elders, PJM;Fernandez, C;Festa, A;Fitipaldi, H;Froguel, P;Gudmundsdottir, V;Gudnason, V;Gerl, MJ;van der Heijden, AA;Jennings, LL;Hansen, MK;Kim, M;Leclerc, I;Klose, C;Kuznetsov, D;Mansour Aly, D;Mehl, F;Marek, D;Melander, O;Niknejad, A;Ottosson, F;Pavo, I;Duffin, K;Syed, SK;Shaw, JL;Cabrera, O;Pullen, TJ;Simons, K;Solimena, M;Suvitaival, T;Wretlind, A;Rossing, P;Lyssenko, V;Legido Quigley, C;Groop, L;Thorens, B;Franks, PW;Lim, GE;Estall, J;Ibberson, M;Beulens, JWJ;'t Hart, LM;Pearson, ER;Rutter, GA; Identification of biomarkers for glycaemic deterioration in type 2 diabetes Nature communications 2023-05-03 [PMID: 37137910] (Bioassay, Mouse) Bioassay Mouse
Cho, MJ;Lee, HG;Yoon, JW;Kim, GR;Koo, JH;Taneja, R;Edelson, BT;Lee, YJ;Choi, JM; Steady-state memory-phenotype conventional CD4+ T cells exacerbate autoimmune neuroinflammation in a bystander manner via the Bhlhe40/GM-CSF axis Experimental & molecular medicine 2023-05-01 [PMID: 37121980] (Bioassay, Mouse) Bioassay Mouse
Show All 263 Publications.

Reviews for IL-1 beta/IL-1F2 (401-ML) (0)

There are no reviews for IL-1 beta/IL-1F2 (401-ML). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-1 beta/IL-1F2 (401-ML) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-1 beta/IL-1F2 Products

Bioinformatics Tool for IL-1 beta/IL-1F2 (401-ML)

Discover related pathways, diseases and genes to IL-1 beta/IL-1F2 (401-ML). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-1 beta/IL-1F2 (401-ML)

Discover more about diseases related to IL-1 beta/IL-1F2 (401-ML).

Pathways for IL-1 beta/IL-1F2 (401-ML)

View related products by pathway.

PTMs for IL-1 beta/IL-1F2 (401-ML)

Learn more about PTMs related to IL-1 beta/IL-1F2 (401-ML).

Blogs on IL-1 beta/IL-1F2.

The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind ...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Mouse IL-1 beta/IL-1F2 Protein and receive a gift card or discount.


Gene Symbol Il1b