Recombinant Mouse IL-1 beta/IL-1F2 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Mouse IL-1 beta/IL-1F2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 2-10 pg/mL.
Source
E. coli-derived mouse IL-1 beta/IL-1F2 protein
Val118-Ser269, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Il1b
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
401-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-1 beta/IL-1F2 Protein

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65%-78% identity with canine, equine, feline, human, porcine, and rhesus IL-1 beta.

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Gray, P.W. et al. (1986) J. Immunol. 137:3644.
  6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

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401-ML
Species: Mu
Applications: Bioactivity

Publications for IL-1 beta/IL-1F2 (401-ML)(180)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 11 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, ELISA (Standard), ELISA Standard, In Vivo, In vivo, Specificity Control, Tissue Culture, Western Blot.


Filter By Application
Binding Assay
(1)
Bioassay
(112)
Cell Culture
(13)
Differentiation
(1)
ELISA (Standard)
(8)
ELISA Standard
(1)
In Vivo
(38)
In vivo
(1)
Specificity Control
(1)
Tissue Culture
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(170)
Rat
(2)
N/A
(5)
All Species
Showing Publications 1 - 10 of 180. Show All 180 Publications.
Publications using 401-ML Applications Species
T Osthues, B Zimmer, V Rimola, K Klann, K Schilling, P Mathoor, C Angioni, A Weigert, G Geisslinge, C Münch, K Scholich, M Sisignano The Lipid Receptor G2A (GPR132) Mediates Macrophage Migration in Nerve Injury-Induced Neuropathic Pain Cells, 2020;9(7):. 2020 [PMID: 32708184] (Bioassay, Mouse) Bioassay Mouse
Y Dong, S Li, Y Lu, X Li, Y Liao, Z Peng, Y Li, L Hou, Z Yuan, J Cheng Stress-induced NLRP3 inflammasome activation negatively regulates fear memory in mice J Neuroinflammation, 2020;17(1):205. 2020 [PMID: 32635937] (In Vivo, Mouse) In Vivo Mouse
EM Hanschmann, SF Petry, S Eitner, CC Maresch, N Lingwal, CH Lillig, T Linn Paracrine regulation and improvement of &amp;beta-cell function by thioredoxin Redox Biol, 2020;34(0):101570. 2020 [PMID: 32473461] (Cell Culture, Mouse) Cell Culture Mouse
B Hou, Y Zhang, P Liang, Y He, B Peng, W Liu, S Han, J Yin, X He Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype Cell Death Dis, 2020;11(5):377. 2020 [PMID: 32415059] (Bioassay, Mouse) Bioassay Mouse
Y Huang, H Wang, Y Hao, H Lin, M Dong, J Ye, L Song, Y Wang, Q Li, B Shan, Y Jiang, H Li, Z Shao, G Kroemer, H Zhang, L Bai, T Jin, C Wang, Y Ma, Y Cai, C Ding, S Liu, Y Pan, W Jiang, R Zhou Myeloid PTEN promotes chemotherapy-induced NLRP3-inflammasome activation and antitumour immunity Nat. Cell Biol., 2020;0(0):. 2020 [PMID: 32367047] (Bioassay, Mouse) Bioassay Mouse
S Ma, A Zhang, X Li, S Zhang, S Liu, H Zhao, S Wu, L Chen, C Ma, H Zhao MiR-21-5p regulates extracellular matrix degradation and angiogenesis in TMJOA by targeting Spry1 Arthritis Res. Ther., 2020;22(1):99. 2020 [PMID: 32357909] (Bioassay, Mouse) Bioassay Mouse
SY Chien, CH Tsai, SC Liu, CC Huang, TH Lin, YZ Yang, CH Tang Noggin Inhibits IL-1&amp;beta and BMP-2 Expression, and Attenuates Cartilage Degeneration and Subchondral Bone Destruction in Experimental Osteoarthritis Cells, 2020;9(4):. 2020 [PMID: 32290085] (Cell Culture, Mouse) Cell Culture Mouse
O Haidar, N O'Neill, CA Staunton, S Bavan, F O'Brien, S Zouggari, U Sharif, A Mobasheri, K Kumagai, R Barrett-Jo Pro-inflammatory Cytokines Drive Deregulation of Potassium Channel Expression in Primary Synovial Fibroblasts Front Physiol, 2020;11(0):226. 2020 [PMID: 32265733] (Bioassay, Mouse) Bioassay Mouse
T Shimo, H Takebe, T Okui, Y Kunisada, S Ibaragi, K Obata, N Kurio, K Shamsoon, S Fujii, A Hosoya, K Irie, A Sasaki, M Iwamoto Expression and Role of IL-1&amp;beta Signaling in Chondrocytes Associated with Retinoid Signaling during Fracture Healing Int J Mol Sci, 2020;21(7):. 2020 [PMID: 32235405] (Bioassay, Mouse) Bioassay Mouse
HS Son, J Lee, HI Lee, N Kim, YJ Jo, GR Lee, SE Hong, M Kwon, NY Kim, HJ Kim, JH Park, SY Lee, W Jeong Benzydamine inhibits osteoclast differentiation and bone resorption via down-regulation of interleukin-1 &amp;beta expression Acta Pharm Sin B, 2020;10(3):462-474. 2020 [PMID: 32140392] (Cell Culture, Mouse) Cell Culture Mouse
Show All 180 Publications.

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Blogs on IL-1 beta/IL-1F2.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

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Bioinformatics

Gene Symbol Il1b
Uniprot