Recombinant Mouse IGFBP-rp1/IGFBP-7 Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Bind
Format
Carrier-Free

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Recombinant Mouse IGFBP-rp1/IGFBP-7 Protein, CF Summary

Details of Functionality
Measured by its ability to bind 6Ckine/CCL21 in a functional ELISA. Nagakubo, D. et al. (2003) J. Immunol. 171:553. When rmIGFBP-rp1/IGFBP-7 is immobilized at 500 ng/mL (100 µL/well), the concentration of Recombinant Human CCL21/6Ckine (Catalog # 366-6C) that produces 50% optimal binding response is found to be approximately 4‑20 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (stably transfected)-derived
Ser28-Leu281, with an N-terminal 10-His tag
Accession #
N-terminal Sequence
His
Protein/Peptide Type
Recombinant Proteins
Gene
Igfbp7
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
27.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
35 kDa, reducing conditions
Publications
Read Publications using
2120-B7 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IGFBP-rp1/IGFBP-7 Protein, CF

  • angiomodulin
  • FSTL2
  • IBP-7
  • IGFBP7
  • IGFBP-7
  • IGFBP-7PGI2-stimulating factor
  • IGFBP-7v
  • IGFBPrp1
  • IGFBP-rp1
  • insulin-like growth factor binding protein 7
  • insulin-like growth factor-binding protein 7
  • MAC25 protein
  • Mac25
  • MAC25IGF-binding protein 7
  • Prostacyclin-stimulating factor
  • PSF
  • PSFAGM
  • TAF
  • Tumor-derived adhesion factor

Background

Insulin-like growth factor (IGF) binding protein-related protein 1 (IGFBP-rp1), also known as IGF binding protein-7 (IGFBP-7) and Mac25 is a 31 - 37 kDa secreted glycoprotein that belongs to the IGFBP superfamily of molecules (1, 2). Members of this superfamily are characterized by the presence of 10 ‑ 12 conserved cysteines in the first third of the molecule, and the partial conservation of an xCGCCxxC octapeptide motif that exists in an N‑terminal IGFBP domain (1, 3). Mouse IGFBP-7 cDNA encodes a 281 amino acid (aa) precursor protein that contains a putative 25 aa signal peptide and a 256 aa mature region. The mature protein contains an N‑terminal 43 aa IGFBP domain that is followed by a 52 aa Kazal-type serine proteinase inhibitor domain and a 105 aa C-terminal Ig-like C2‑type domain (4, 5). The molecule is known to contain about 4 kDa of carbohydrate and believed to undergo phosphorylation (6). Mature mouse IGFBP-7 is 95% and 91% aa identical to mature rat and human IGFBP-7, respectively. Cells known to express IGFBP-7 include osteoblasts, select skeletal muscle fibers, visceral and vascular smooth muscle cells, breast epithelium, ciliated epithelium, renal tubular epithelium, astrocytes and oligodendroglia, and vascular endothelium in all tissues except brain (7). IGFBP-7 will bind both IGF-I and insulin, albeit at low affinity. When bound to IGF-I, it participates in growth promoting effects (8). Alternatively, IGFBP-7 by itself is an inhibitor of cell proliferation (6). Thus, its function is unclear. It is known to bind heparin, syndecan-1 and chemokines (8, 9). Based on human studies and aa conservation in relevant regions, mouse IGFBP-7 might be be expected to undergo proteolytic cleavage extracellularly (8). This will create an 8 kDa, 72 aa subunit (containing the IGFBP domain) that is disulfide-linked to a 25 kDa, 184 aa C-terminal subunit. Cleavage will reduce IGF‑I bonding activity and the promotion of IGF‑dependent cell proliferation.

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Bioinformatics

Gene Symbol Igfbp7
Entrez
Uniprot