Recombinant Mouse IGFBP-2 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse IGFBP-2 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.125‑0.5 µg/mL in the presence of 30 ng/mL rmIGF-II.
Source
Mouse myeloma cell line, NS0-derived mouse IGFBP-2 protein
Gln35-Gln305
Accession #
N-terminal Sequence
Gln35
Protein/Peptide Type
Recombinant Proteins
Gene
Igfbp2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
35 kDa, reducing conditions
Publications
Read Publications using
797-B2 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IGFBP-2 Protein, CF

  • BP2
  • IBP2
  • IBP-2
  • IGF-binding protein 2
  • IGFBP2
  • IGFBP-2
  • IGF-BP53
  • insulin-like growth factor binding protein 2 (36kD)
  • insulin-like growth factor binding protein 2, 36kDa
  • insulin-like growth factor-binding protein 2

Background

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Mouse IGFBP-2 cDNA encodes a 305 amino acid (aa) residue precursor protein with a putative 34 aa residue signal peptide that is processed to generate the 271 aa residue mature protein. Mouse and human IGFBP-2 share approximately 82% homology. IGFBP-2 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues. IGFBP-2 binds preferentially to IGF II, exhibiting a 2 - 10 fold higher affinity for IGF II than for IGF I.

  1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
  2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
  3. Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57.
  4. Landwehr, J. et al. (1993) Gene 124:281.

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Publications for IGFBP-2 (797-B2)(5)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 3 applications: Bioassay, ELISA Standard, In Vivo.


Filter By Application
Bioassay
(2)
ELISA Standard
(2)
In Vivo
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
N/A
(2)
All Species
Showing Publications 1 - 5 of 5.
Publications using 797-B2 Applications Species
R Ichijo, H Kobayashi, S Yoneda, Y Iizuka, H Kubo, S Matsumura, S Kitano, H Miyachi, T Honda, F Toyoshima Tbx3-dependent amplifying stem cell progeny drives interfollicular epidermal expansion during pregnancy and regeneration Nat Commun, 2017;8(1):508. 2017 [PMID: 28894084] (In Vivo, Mouse) In Vivo Mouse
Hellbach N, Weise S, Vezzali R, Wahane S, Heidrich S, Roidl D, Pruszak J, Esser J, Vogel T Neural deletion of Tgfbr2 impairs angiogenesis through an altered secretome. Hum Mol Genet, 2014;23(23):6177-90. 2014 [PMID: 24990151] (Bioassay, Human) Bioassay Human
Yamada PM, Mehta HH, Hwang D Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation. Endocrinology, 2010;151(12):5741-50. 2010 [PMID: 20926583] (ELISA Standard, N/A) ELISA Standard N/A
Villani RM, Adolphe C, Palmer J, Waters MJ, Wainwright BJ Patched1 inhibits epidermal progenitor cell expansion and basal cell carcinoma formation by limiting Igfbp2 activity. Cancer Prev Res (Phila), 2010;3(10):1222-34. 2010 [PMID: 20858761] (Bioassay, Mouse) Bioassay Mouse
Raina K, Blouin MJ, Singh RP, Majeed N, Deep G, Varghese L, Glode LM, Greenberg NM, Hwang D, Cohen P, Pollak MN, Agarwal R Dietary feeding of silibinin inhibits prostate tumor growth and progression in transgenic adenocarcinoma of the mouse prostate model. Cancer Res., 2007;67(22):11083-91. 2007 [PMID: 18006855] (ELISA Standard, N/A) ELISA Standard N/A

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Bioinformatics

Gene Symbol Igfbp2
Entrez
Uniprot