Recombinant Mouse IFN-alpha 1 Protein, CF

Recombinant Mouse IFN-alpha 1 (Catalog # 10148-IF)suppresses viral activity on L-929 mouse fibroblast cells infected withencephalomyocarditis (EMC) virus. The ED50 for this effect is 1.2‑12 pg/mL.

2 μg/lane of Recombinant Mouse IFN-alpha 1 (Catalog # 10148-IF) was resolved withSDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized byCoomassie® Blue staining, showing bands at 18-22 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IFN-alpha 1 Protein, CF Summary

• Details of Functionality: Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is 1.2-12 pg/mL.
• Source: Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 1 protein
  Cys24-Lys189
• Accession #: P01572
• N-terminal Sequence: No results obtained. Cys24 inferred from enzymatic pyroglutamate treatment revealing Asp25.
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 18-22 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-alpha 1 Protein, CF

• IFL;IFN;IFNA@;IFNA13;IFN-ALPHA;IFN-alphaD;Interferon alpha-1/13;leIF D
• IFNA1
• IFNalpha 1
• IFN-alpha 1

Background

The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and classified based on their binding specificity to cell surface receptors (1). The type I IFN bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF-alpha induced signaling (2, 3). Both the human and mouse genome code for more than a dozen closely related IFNa subtypes and the varous IFN a share about 80% sequence homology among them (4-5). Interferon-alpha 1 (IFNA1) is a secreted, approximately 19 kDa member of the type I interferon family of molecules (6). Mature mouse IFN-alpha 1 shares 63% and 82% amino acid sequence identity with human and rat IFN-alpha 1, respectively. Low level IFN-alpha is detected under physiological conditions, and the production of IFNs is markedly enhanced during virus infection (7). Although originally discovered by its capability to fight virus replication, IFN-alpha functions as a prototypic tumor suppressor that represses the clinical tumorigenic phenotype in some malignancies (7).

1. Pestka, S. et al. (1987) Annu Rev Biochem. 56:727.
2. Fung, K.Y. et al. (2013) Science 339:1088.
3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36: 139.
5. van Pesch, V. et al. (2004) J. Virol. 78: 8219.
6. Wijesundara, D.K. et al. (2014) Front. Immunol. 5:412.
7. Gutterman J. U. et al. (1994) Proc. Natl. Acad. Sci. U. S. A. 91:1198.

Publications for IFN-alpha 1 (10148-IF)(1)

Publication using 10148-IF

• GM Wiedemann, EK Santosa, S Grassmann, S Sheppard, JB Le Luduec, NM Adams, C Dang, KC Hsu, JC Sun, CM Lau Deconvoluting global cytokine signaling networks in natural killer cells Nature Immunology, 2021-04-15;0(0):. 2021-04-15 [PMID: 33859404] (Bioassay, Mouse)

Bioinformatics

• Uniprot:
  • P01572()