Recombinant Mouse HGFR/c-MET Fc Chimera His-tag Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse HGFR/c-MET Fc Chimera His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to bind rmHGF in a functional ELISA with an estimated
  K_D <0.2 nM.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse HGF R/c-MET protein
  

  Mouse HGF R (Met1 - Asn929) Accession # P16056	DIEGRMD	Human IgG1 (Pro100 - Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: P16056
• N-terminal Sequence: Glu25 ( alpha chain) & Ser307 ( beta chain)
• Structure / Form: Tetramer; disulfide-linked homodimer of disulfide-linked heterodimers
• Protein/Peptide Type: Recombinant Proteins
• Gene: Met
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 32 kDa ( alpha chain) & 96 kDa ( beta chain) (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 35-40 kDa & 100-110 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse HGFR/c-MET Fc Chimera His-tag Protein, CF

• AUTS9
• cMET
• c-MET
• EC 2.7.10
• EC 2.7.10.1
• hepatocyte growth factor receptor
• HGF R
• HGF receptor
• HGF/SF receptor
• HGFR
• Met (c-Met)
• met proto-oncogene (hepatocyte growth factor receptor)
• met proto-oncogene tyrosine kinase
• MET
• oncogene MET
• Proto-oncogene c-Met
• RCCP2
• Scatter factor receptor
• SF receptor
• Tyrosine-protein kinase Met

Background

HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular  alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). An alternately spliced form of mouse HGF R lacks a cytoplasmic juxtamembrane region important for regulation of signal transduction (5, 6). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 7). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (8, 9). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (10). In the absence of ligand, HGF R forms noncovalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, integrin  alpha 6/ beta 4, plexins B1, 2, 3, and MSP R/Ron (11 - 18). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (11 - 18). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (11, 15, 16). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (19). Genetic polymorphisms, chromosomal translocation, overexpression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, mouse HGF R shares 87%, 87%, and 94% amino acid sequence identity with canine, human, and rat HGF R, respectively.

1. Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
3. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
4. Chan, A.M. et al. (1988) Oncogene 2:593.
5. Lee, C.-C. and K.M. Yamada (1994) J. Biol. Chem. 269:19457.
6. Lee, C.-C., et al. (1995) J. Biol. Chem. 270:507.
7. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
8. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
9. Ponzetto, C. et al. (1994) Cell 77:261.
10. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
11. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
12. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
13. Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
14. Wang, X. et al. (2002) Mol. Cell 9:411.
15. Trusolino, L. et al. (2001) Cell 107:643.
16. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
17. Conrotto, P. et al. (2004) Oncogene 23:5131.
18. Follenzi, A. et al. (2000) Oncogene 19:3041.
19. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.

Publications for HGFR/c-MET (527-ME)(3)

Publications using 527-ME

• DK Yoo, SR Lee, Y Jung, H Han, HK Lee, J Han, S Kim, J Chae, T Ryu, J Chung Machine Learning-Guided Prediction of Antigen-Reactive In Silico Clonotypes Based on Changes in Clonal Abundance through Bio-Panning Biomolecules, 2020-03-08;10(3):. 2020-03-08 [PMID: 32182714] (In Vivo, Gallus domesticus (Chicken))
• Lee Y, Park H, Woo S, Park E, Kang J RhoA/phosphatidylinositol 3-kinase/protein kinase B/mitogen-activated protein kinase signaling after growth arrest-specific protein 6/mer receptor tyrosine kinase engagement promotes epithelial cell growth and wound repair via upregulation of hepatocyte growth factor in macrophages. J Pharmacol Exp Ther, 2014-06-17;350(3):563-77. 2014-06-17 [PMID: 24939420] (Bioassay, Mouse)
• Tatsumi R, Wuollet AL, Tabata K, Nishimura S, Tabata S, Mizunoya W, Ikeuchi Y, Allen RE A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation. Am. J. Physiol., Cell Physiol., 2009-01-21;296(4):C922-9. 2009-01-21 [PMID: 19158401] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: Met
• Uniprot:
  • P16056()