Recombinant Mouse Gas6 (Full Length) Protein, CF

Recombinant Mouse Gas6 (Catalog # 8310-GS) down regulates the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 20-100 ng/mL.

1 μg/lane ofRecombinant Mouse Gas6 (Full Length, Catalog # 8310-GS) was resolved with SDS-PAGEunder reducing (R) and non-reducing (NR) conditions and visualized by silverstaining, showing R bands at 85.4 and 69.2 kDa and NR band at 64.4 kDa.

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Gas6 (Full Length) Protein, CF Summary

• Details of Functionality: Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703. The ED₅₀ for this effect is 20-100 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Gas6 protein
  Ala46-Pro673, with a C-terminal 6-His tag
• Accession #: CAA42507
• N-terminal Sequence: Ala46 & Tyr47
• Protein/Peptide Type: Recombinant Proteins
• Gene: Gas6
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 78-94 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Gas6 (Full Length) Protein, CF

• AXLLG
• AXLLGAXL stimulatory factor
• AXSFAXL receptor tyrosine kinase ligand
• DKFZp666G247
• FLJ34709
• Gas6
• GAS-6
• growth arrest-specific 6
• growth arrest-specific protein 6

Background

Gas6 (Growth Arrest Specific 6) is a secreted 75 kDa multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest. It is expressed by endothelial cells, fibroblasts, neurons, smooth muscle cells, and platelets, and it plays a role in vascular, thrombotic, atherosclerotic, inflammatory, autoimmune, renal, and cancer pathologies (1, 2). Both Gas6 and the related Protein S contain an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and two C-terminal Laminin G-like domains. And like Protein S, Gas6 is dependent upon vitamin K for activity. Within the Gla, EGF-like, and Laminin G-like domains, mouse Gas6 shares 83%, 94%, and 41% aa sequence identity with the equivalent regions in human Gas6, rat Gas6, and mouse Protein S, respectively. Gas6 binds to and induces signaling through the receptor tyrosine kinase TAM subfamily, which includes Axl, Dtk/Tyro3, and Mer (3). Shed soluble forms of Axl and Mer retain the ability to bind Gas6 and function as decoy receptors (4, 5). Gas6 participates in tissue homeostasis by protecting cells from stress-induced apoptosis and promoting apoptotic cell phagocytosis. The affinity of the gamma -carboxylated Gla domain for phosphatidylserine contributes to the role of Gas6 in phagocytosis as well as the cellular entry of select viruses (6, 7). Gas6 can function as a pro-inflammatory molecule by promoting platelet activation (8, 9) and can also inhibit inflammatory cytokine production from monocytes, macrophages, and microglia (10). In addition, Gas6 induces the proliferation of cardiac fibroblasts, Schwann cells, vascular smooth muscle cells, and the differentiation of NK cell precursors (11-14). It also inhibits VEGF-induced angiogenesis (15) and can have either positive or negative effects on tumor cell proliferation and invasion (16, 17).

1. Laurance, S. et al. (2012) Adv. Nutr. 3:196.
2. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
3. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
4. Sather, S. et al. (2007) Blood 109:1026.
5. Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.
6. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
7. Morizono, K. et al. (2011) Cell Host Microbe 9:286.
8. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
9. Robins, R.S. et al. (2012) Blood 121:692.
10. Alciato, F. et al. (2010) J. Leukoc. Biol. 87:869.
11. Stenhoff, J. et al. (2004) Biochem. Biophys. Res. Commun. 319:871.
12. Li, R. et al. (1996) J. Neurosci. 16:2012.
13. Nakano, T. et al. (1995) J. Biol. Chem. 270:5702.
14. Caraux, A. et al. (2006) Nat. Immunol. 7:747.
15. Gallicchio, M. et al. (2005) Blood 105:1970.
16. Loges, S. et al. (2010) Blood 115:2264.
17. Gustafsson, A. et al. (2009) PLoS ONE 4:e7575.

Publications for Gas6 (8310-GS)(5)

Publications using 8310-GS

• Fang, Q;Yan, Q;Liu, X;Zhang, X;Zha, L;Zhang, R;Gao, Z;Du, J;Chen, L; Activated AXL Ameliorates Alcohol-associated Steatotic Liver Ischemia-Reperfusion Injury by Inhibiting ER stress and Mitochondria-associated Apoptosis International journal of biological sciences 2025-01-20 [PMID: 39897049] (In vivo assay, Mouse)
• ME Fane, Y Chhabra, GM Alicea, DA Maranto, SM Douglass, MR Webster, VW Rebecca, GE Marino, F Almeida, BL Ecker, DJ Zabransky, L Hüser, T Beer, HY Tang, A Kossenkov, M Herlyn, DW Speicher, W Xu, X Xu, EM Jaffee, JA Aguirre-Gh, AT Weeraratna Stromal changes in the aged lung induce an emergence from melanoma dormancy Nature, 2022-06-01;606(7913):396-405. 2022-06-01 [PMID: 35650435] (Bioassay, Mouse)
• L Salmi, F Gavelli, F Patrucco, M Bellan, PP Sainaghi, GC Avanzi, LM Castello Growth Arrest-Specific Gene 6 Administration Ameliorates Sepsis-Induced Organ Damage in Mice and Reduces ROS Formation In Vitro Cells, 2021-03-09;10(3):. 2021-03-09 [PMID: 33803290] (Bioassay, Mouse)
• S Choi, B Zhang, S Ma, M Gonzalez-C, D Stein, X Jin, ST Kim, YL Kang, A Besnard, A Rezza, L Grisanti, JD Buenrostro, M Rendl, M Nahrendorf, A Sahay, YC Hsu Corticosterone inhibits GAS6 to govern hair follicle stem-cell quiescence Nature, 2021-03-31;0(0):. 2021-03-31 [PMID: 33790465] (Bioassay, Mouse)
• GJ Zhao, JY Zheng, JL Bian, LW Chen, N Dong, Y Yu, GL Hong, A Chandoo, YM Yao, ZQ Lu Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4(+)CD25(+) Regulatory T Cells Mainly through Axl Receptor Mediators Inflamm, 2017-02-08;2017(0):6848430. 2017-02-08 [PMID: 28270700] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: Gas6
• Uniprot:
  • CAA42507()