Recombinant Mouse Erythropoietin/EPO Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse Erythropoietin/EPO Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.4‑2 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Erythropoietin/EPO protein
Ala27-Arg192
Accession #
N-terminal Sequence
Ala27
Protein/Peptide Type
Recombinant Proteins
Gene
Epo
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
18.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
36 kDa, reducing conditions
Publications
Read Publications using
959-ME in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Erythropoietin/EPO Protein

  • ECYT5
  • EP
  • EPO
  • epoetin
  • Erythropoietin
  • MGC138142
  • MVCD2

Background

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of (EPO is required for biological activities in vivo (4). Mature mouse (EPO shares 95% amino acid sequence identity with rat (EPO and 73%-82% with bovine, canine, equine, feline, human, ovine, and porcine EPO. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6-8). (EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the (EPO receptor (EPO R) (8, 9). (EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of (EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating (EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce (EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). (EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).

  1. Koury, M. J. (2005) Exp. Hematol. 33:1263.
  2. Shoemaker, C.B. and L.D. Mitsock (1986) Mol. Cell. Biol 6:849.
  3. Wen, D. et al. (1993) Blood 82:1507.
  4. Tsuda E. et al. (1990) Eur. J. Biochem. 188:405.
  5. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
  6. Eckardt, K. U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
  7. Sharples, E. J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
  8. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
  9. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
  10. Acs, G. et al. (2001) Cancer Res. 61:3561.
  11. Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
  12. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
  13. Kertesz, N. et al. (2004) Dev. Biol. 276:101.

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Applications: Bioactivity

Publications for Erythropoietin/EPO (959-ME)(16)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Bioassay, ELISA Developmet, In Vivo, Multiplex Standard.


Filter By Application
Bioassay
(13)
ELISA Developmet
(1)
In Vivo
(1)
Multiplex Standard
(1)
All Applications
Filter By Species
Mouse
(16)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using 959-ME Applications Species
JM Bertho, D Kereselidz, L Manens, C Culeux, V Magneron, J Surette, M Blimkie, L Bertrand, H Wyatt, M Souidi, I Dublineau, N Priest, JR Jourdain Hto, Tritiated Amino Acid Exposure and External Exposure Induce Differential Effects on Hematopoiesis and Iron Metabolism Sci Rep, 2019;9(1):19919. 2019 [PMID: 31882739] (Multiplex Standard, Mouse) Multiplex Standard Mouse
S Mayers, PD Moço, T Maqbool, PN Silva, DM Kilkenny, J Audet Establishment of an erythroid progenitor cell line capable of enucleation achieved with an inducible c-Myc vector BMC Biotechnol., 2019;19(1):21. 2019 [PMID: 30987611] (Bioassay, Mouse) Bioassay Mouse
N Funk, M Munz, T Ott, K Brockmann, A Wenninger-, R Kühn, D Vogt-Weise, F Giesert, W Wurst, T Gasser, S Biskup The Parkinson&#039;s disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4 Sci Rep, 2019;9(1):4515. 2019 [PMID: 30872638] (Bioassay, Mouse) Bioassay Mouse
M Yashiro, M Ohya, T Mima, Y Ueda, Y Nakashima, K Kawakami, Y Ishizawa, S Yamamoto, S Kobayashi, T Yano, Y Tanaka, K Okuda, T Sonou, T Shoshihara, Y Iwashita, Y Iwashita, K Tatsuta, R Matoba, S Negi, T Shigematsu FGF23 modulates the effects of erythropoietin on gene expression in renal epithelial cells Int J Nephrol Renovasc Dis, 2018;11(0):125-136. 2018 [PMID: 29670389] (Bioassay, Mouse) Bioassay Mouse
T Xu, H Jin, Y Lao, P Wang, S Zhang, H Ruan, Q Mao, L Zhou, L Xiao, P Tong, C Wu Administration of erythropoietin prevents bone loss in osteonecrosis of the femoral head in mice Mol Med Rep, 2017;0(0):. 2017 [PMID: 29039481] (In Vivo, Mouse) In Vivo Mouse
T Takagishi, M Takehara, S Seike, K Miyamoto, K Kobayashi, M Nagahama Clostridium perfringens ?-toxin impairs erythropoiesis by inhibition of erythroid differentiation Sci Rep, 2017;7(1):5217. 2017 [PMID: 28701754] (Bioassay, Mouse) Bioassay Mouse
A Laroque, G Min-Oo, M Tam, P Ponka, MM Stevenson, P Gros The mouse Char10 locus regulates severity of pyruvate kinase deficiency and susceptibility to malaria PLoS ONE, 2017;12(5):e0177818. 2017 [PMID: 28542307] (Bioassay, Mouse) Bioassay Mouse
P Pathipati, DM Ferriero The Differential Effects of Erythropoietin Exposure to Oxidative Stress on Microglia and Astrocytes in vitro Dev. Neurosci., 2017;0(0):. 2017 [PMID: 28511187] (Bioassay, Mouse) Bioassay Mouse
N Pardi, AJ Secreto, X Shan, F Debonera, J Glover, Y Yi, H Muramatsu, H Ni, BL Mui, YK Tam, F Shaheen, RG Collman, K Karikó, GA Danet-Desn, TD Madden, MJ Hope, D Weissman Administration of nucleoside-modified mRNA encoding broadly neutralizing antibody protects humanized mice from HIV-1 challenge Nat Commun, 2017;8(0):14630. 2017 [PMID: 28251988] (ELISA Developmet, Mouse) ELISA Developmet Mouse
Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency Stem Cell Reports, 2016;0(0):. 2016 [PMID: 27453007] (Bioassay, Mouse) Bioassay Mouse
Show All 16 Publications.

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Bioinformatics

Gene Symbol Epo
Uniprot