Recombinant Mouse ErbB4/Her4 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse ErbB4/Her4 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the biological activity of Neuregulin-1-beta 1 on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED₅₀ for this effect is 3-12 µg/mL in the presence of 10 ng/mL of Recombinant Human NRG1‑ beta 1/HRG1‑ beta 1 Extracellular Domain (Catalog # 377-HB).
• Source: Mouse myeloma cell line, NS0-derived mouse ErbB4/Her4 protein
  

  Mouse ErbB4 (Gln26 - Val657) Accession #Q61527	IEGRMDP	Mouse IgG2A (Glu98 - Lys330)
  N-terminus		C-terminus

• Accession #: Q61527
• N-terminal Sequence: No results obtained: Gln26 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: Erbb4
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 97.5 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 120-140 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ErbB4/Her4 Fc Chimera Protein, CF

• avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4
• EC 2.7.10
• EC 2.7.10.1
• ErbB4
• HER4
• HER4MGC138404
• p180erbB4
• Proto-oncogene-like protein c-ErbB-4
• receptor tyrosine-protein kinase erbB-4
• Tyrosine kinase-type cell surface receptor HER4
• v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4
• v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)

Background

ErbB4, also called Her4 (human epidermal growth factor receptor 4) in humans, is a type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors named for a viral oncogene (1, 2). ErbB family members serve as receptors for the EGF family of growth factors (1, 2). Mouse ErbB4 contains a 25 amino acid (aa) signal sequence, a 626 aa extracellular domain (ECD), a 24 aa transmembrane region, and a 617 aa cytoplasmic domain (3). Several ErbB4 isoforms exist (4 - 6). Isoforms JM-a, b, and d contain extracellular juxtamembrane aa insertions ranging from 13 - 36 aa in length. Isoform CYT-1 contains a cytoplasmic PI3K binding site absent in CYT-2. The mouse ECD (JM-a isoform) shares 99%, 99%, 98%, 96%, 95%, 91% and 80% aa identity with human, rat, equine, opossum, platypus, chicken and Xenopus ErbB4, respectively. Isoforms JM-a and JM-d can be proteolytically cleaved by TACE/ADAM-17 (TNF-alpha converting enzyme) upon ligand binding (7). Following release of the ECD, the cytoplasmic portion is further cleaved by a gamma -secretase and translocated to the nucleus (8). Recombinant ECD is able to compete with cellular ErbB4 for ligands (9). After ligand binding, the soluble ECD forms homodimers, and then may form hetero-multimers with ErbB2 (10, 11). ErbB4 ligands include the neuregulins, some of which (NRG-1 and -2) also bind ErbB3. Other ligands such as epiregulin, betacellulin and heparin-binding EGF-like growth factor (HB-EGF) are shared by ErbB1 (1, 12). ErbB4 is expressed in the heart (JM-b isoform only), kidney (JM-a isoform only), pituitary, brain and breast (1, 2, 13). It plays important roles in heart, breast and neuronal development (13). In human ErbB4 participates in the NRG-1-mediated downregulation of NMDA receptor activity that occurs in schizophrenia (14).

1. Linggi, B. and G. Carpenter (2006) Trends Cell Biol. 16:649. 
2. Citri, A. and Y. Yarden (2006) Nat. Rev. Mol. Cell Biol. 7:505. 
3. Swissprot Accession # Q61527.
4. Elenius, K. et al. (1997) J. Biol. Chem. 272:26761. 
5. Gilmour, L.M.R. et al. (2001) Cancer Res. 61:2169. 
6. Elenius, K. et al. (1999) Oncogene 18:2607. 
7. Rio, C. et al. (2000) J. Biol. Chem. 275:10379. 
8. Ni, C-Y. et al. (2001) Science 294:2179.
9. Gilmore, J.L. and D.J. Riese II (2004) Oncol. Res. 14:589.
10. Bouyain, S. et al. (2005) Proc. Natl. Acad. Sci. USA 102:15024.
11. Ferguson, K.M. et al. (2000) EMBO J. 16:4632.
12. Sundaresan, S. et al. (1998) Endocrinology 139:4756.
13. Jones, F.E. et al. Cell Cycle 2:555.
14. Hahn C-G. et al. (2006) Nat. Med. 12:824.

Bioinformatics

• Gene Symbol: Erbb4
• Uniprot:
  • Q61527()