Recombinant Mouse Ephrin-A2 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Ephrin-A2 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized recombinant rat EphA5 Fc Chimera at 2 µg/mL (100 µL/well) can bind rmEphrin-A2/Fc Chimera with a linear range of 0.16‑10 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Ephrin-A2 protein
  

  Mouse Ephrin-A2 (Glu23-Asn184) Accession # P52801	IEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: P52801
• N-terminal Sequence: Glu23
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Efna2
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 46 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 57 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-A2 Fc Chimera Protein, CF

• Cek7-L
• EFNA2
• ELF-1
• EPH-related receptor tyrosine kinase ligand 6
• EphrinA2
• Ephrin-A2
• EPLG6HEK7 ligand
• HEK7-L
• HEK7-ligand
• LERK6
• LERK6LERK-6
• ligand of eph-related kinase 6

Background

Ephrin-A2, also known as ELF-1, HEK7-L, LERK-6, and EPLG6, is an approximately 20 kDa member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-A2 preferentially interacts with receptors in the EphA family (1, 2). Mouse Ephrin-A2 is synthesized with a 20 amino acid (aa) signal peptide, a 164 aa mature chain, and a 25 aa C-terminal propeptide which is removed prior to GPI linkage of Ephrin-A2 to the membrane (3, 4). It shares 93% and 100% aa sequence identity with human and rat Ephrin-A2, respectively. Ephrin-A2 is expressed in discrete regions of the developing nervous system and limb buds (4 - 7). Its distribution complements the pattern of Eph receptor expression, and this plays an important role in tissue morphogenesis (7‑9). Ephrin-A2 exerts an axon repulsive signal which is important for the accurate pathfinding of retinal ganglion cell axons to the tectum and hippocampal axons to the lateral septum (8, 10). Its up‑regulation in astrocytes at sites of optic nerve damage may prevent re-innervation by retinal ganglion cells (11). Ephrin-A2 is also expressed on neural progenitor cells in the subventricular zone (SVZ). It interacts with EphA7, triggering reverse signaling through Ephrin-A2 and inhibition of progenitor cell proliferation (9). In the developing limbs, Ephrin-A2 regulates cartilage morphogenesis and the projection of motoneuron axons (6, 7, 12).

1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
3. Shao, H. et al. (1995) J. Biol. Chem. 270:3467.
4. Cheng, H.-J. et al. (1994) Cell 79:157.
5. Kenmuir, C.L. et al. (2012) Anat. Rec. (Hoboken) 295:105.
6. Ohta, K. et al. (1997) Mech. Dev. 64:127.
7. Wada, N. et al. (2003) Dev. Biol. 264:550.
8. Gao, P.-P. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11161.
9. Holmberg, J. et al. (2005) Genes Dev. 19:462.
10. Nakamoto, M. et al. (1996) Cell 86:755.
11. Symonds, A.C.E. et al. (2007) Eur. J. Neurosci. 25:744.
12. Eberhart, J. et al. (2000) Dev. Neurosci. 22:237.

Publications for Ephrin-A2 (603-A2)(7)

Publications using 603-A2

• ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus Proc. Natl. Acad. Sci. U.S.A., 2019-09-23;0(0):. 2019-09-23 [PMID: 31548390] (Coprecipitation Assay, Virus - Henipavirus)
• Kabayama H, Takeuchi M, Taniguchi M Syntaxin 1B suppresses macropinocytosis and semaphorin 3A-induced growth cone collapse. J. Neurosci., 2011-05-18;31(20):7357-64. 2011-05-18 [PMID: 21593320] (Bioassay, Chicken)
• Dunaway CM, Hwang Y, Lindsley CW Cooperative signaling between Slit2 and Ephrin-A1 regulates a balance between angiogenesis and angiostasis. Mol. Cell. Biol., 2010-12-06;31(3):404-16. 2010-12-06 [PMID: 21135133] (Immunoprecipitation, Mouse)
• Genander M, Holmberg J, Frisen J Ephrins negatively regulate cell proliferation in the epidermis and hair follicle. Stem Cells, 2010-07-01;28(7):1196-205. 2010-07-01 [PMID: 20506314] (In Vivo, Mouse)
• Fitzgerald M, Buckley A, Lukehurst SS, Dunlop SA, Beazley LD, Rodger J Neurite responses to ephrin-A5 modulated by BDNF: evidence for TrkB-EphA interactions. Biochem. Biophys. Res. Commun., 2008-07-30;374(4):625-30. 2008-07-30 [PMID: 18674513] (Surface Plasmon Resonance, Human)
• Hendricks M, Mathuru AS, Wang H, Silander O, Kee MZ, Jesuthasan S Disruption of Esrom and Ryk identifies the roof plate boundary as an intermediate target for commissure formation. Mol. Cell. Neurosci., 2007-10-16;37(2):271-83. 2007-10-16 [PMID: 18060805] (IHC, Zebrafish)
• Sharfe N, Freywald A, Toro A, Dadi H, Roifman C Ephrin stimulation modulates T cell chemotaxis. Eur. J. Immunol., 2002-12-01;32(12):3745-55. 2002-12-01 [PMID: 12516569] (Bioassay, Human)

Bioinformatics

• Gene Symbol: Efna2
• Uniprot:
  • P52801()