Recombinant Mouse EGF-L6 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse EGF-L6 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. Osada, A. et al. (2005) Exp. Cell Res. 303:148. Recombinant Mouse EGF-L6 immobilized at 2 µg/mL, 100 µL/well, will mediate >60% NIH/3T3 cell adhesion (100 µL/well at 5 x 10⁵ cells/mL).
• Source: Mouse myeloma cell line, NS0-derived mouse EGF-L6 protein
  Thr287-Gly550 & Lys291-Gly550, both with a C-terminal 6-His tag
• Accession #: Q9JJZ5
• N-terminal Sequence: Thr287 & Lys291
• Protein/Peptide Type: Recombinant Proteins
• Gene: Egfl6
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 37-40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse EGF-L6 Protein, CF

• DKFZp564P2063
• EGF repeat-containing protein 6
• EGFL6
• EGF-L6
• EGF-like protein 6
• EGF-like-domain, multiple 6
• epidermal growth factor-like protein 6
• MAEG
• MAM and EGF domain containing
• MAM and EGF domains-containing gene protein
• W80

Background

Epidermal Growth Factor-like Domain, Multiple 6 (EGF-L6), also known as MAM- and EGF-containing (MAEG) is a member of the EGF repeat superfamily of proteins. This secreted protein contains five EGF-like repeats, an RGD motif that binds to Integrin alpha 8 beta 1, and a C-terminal MAM domain (1, 2). Within shared regions, mouse EGF-L6 shares 75% and 91% amino acid sequence identity with the human and rat orthologs, respectively. EGF-L6 is expressed in a multitude of fetal tissues during early development such as lung, heart, liver, spleen, cochlea, and placenta (3, 4). It is also expressed in the dermatome and dermatome-derived tissues including trunk dermis, hair follicles, and cranio-facial mesenchyme (5). EGF-L6 is expressed by osteoblastic-like cells and has been shown to act in a paracrine manner to promote migration of vascular endothelial cells, thus regulating angiogenesis in the local environment of the bone (4, 6). Additionally, EGF-L6 is expressed by epidermal stem cells in the hair follicle bulge and is believed to play a role in regulating the dermal vasculature (7). EGF-L6 is also produced by mature adipocytes and is thought to be involved in the process of adipose tissue expansion and the development of obesity (8). EGF-L6 expression is increased in fibroblastic meningioma and ovarian cancer and has been proposed as a biomarker for the latter (9, 10).

1. Buchner, G. et al. (2000) Genomics 65:16.
2. Osada, A. et al. (2005) Exp. Cell Res. 303:148.
3. Yeung, G. et al. (1999) Genomics 62:304.
4. Chim, S.M. et al. (2011) J. Biol. Chem. 286:22035.
5. Buchner, G. et al. (2000) Mech. Dev. 98:179.
6. Chim, S.M. et al. (2013) Cytokine Growth Factor Rev. 24:297.
7. Xiao, Y. et al. (2013) J. Invest. Dermatol. 133:2324.
8. Oberauer, R. et al. (2010) Mol. Cell. Biochem. 343:257.
9. Buckanovich, R.J. et al. (2007) J. Clin. Oncol. 25:852.
10. Wang, X. et al. (2012) PLoS ONE 7:e52707.

Bioinformatics

• Gene Symbol: Egfl6
• Uniprot:
  • Q9JJZ5()