Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF


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Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to mouse E-Selectin/Fc Chimera coated plates (2 µg/mL with 100 µL/well), approximately 85-100% will adhere for 1 hour incubation at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Mouse myeloma cell line, NS0-derived
Mouse E-Selectin
Accession # Q00690
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
85.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
110 - 130 kDa, reducing conditions
Read Publications using
575-ES in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile, deionized water.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse E-Selectin/CD62E Fc Chimera Protein, CF

  • CD62 antigen-like family member E
  • CD62E antigen
  • CD62E
  • ELAM
  • ELAM1
  • ELAM-1
  • ELAM1E-selectin
  • endothelial adhesion molecule 1
  • Endothelial leukocyte adhesion molecule 1
  • ESEL
  • E-Selectin
  • LECAM2
  • leukocyte endothelial cell adhesion molecule 2
  • Leukocyte-endothelial cell adhesion molecule 2
  • SELE
  • selectin E


E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E‑Selectin proteins share 81% amino acid similarity.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

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Publications for E-Selectin/CD62E (575-ES)(29)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Binding Assay, Bioassay, Flow, In Vivo, WB Detection.

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In Vivo
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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using 575-ES Applications Species
C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression Sci Rep, 2018;8(1):420. 2018 [PMID: 29323143] (Bioassay, Human) Bioassay Human
BR Gittens, JV Bodkin, S Nourshargh, M Perretti, D Cooper Galectin-3: A Positive Regulator of Leukocyte Recruitment in the Inflamed Microcirculation J. Immunol., 2017;0(0):. 2017 [PMID: 28438899] (Bioassay, Mouse) Bioassay Mouse
Y Pan, T Tian, CO Park, SY Lofftus, S Mei, X Liu, C Luo, JT O'Malley, A Gehad, JE Teague, SJ Divito, R Fuhlbrigge, P Puigserver, JG Krueger, GS Hotamislig, RA Clark, TS Kupper Survival of tissue-resident memory T cells requires exogenous lipid uptake and metabolism Nature, 2017;0(0):. 2017 [PMID: 28219080] (Bioassay, Mouse) Bioassay Mouse
X Jiang, CO Park, J Geddes Swe, MJ Yoo, O Gaide, TS Kupper Dermal ?� T Cells Do Not Freely Re-Circulate Out of Skin and Produce IL-17 to Promote Neutrophil Infiltration during Primary Contact Hypersensitivity PLoS ONE, 2017;12(1):e0169397. 2017 [PMID: 28081153] (Flow,, Mouse) Flow Mouse
Leukocyte-borne ?(1,3)-fucose is a negative regulator of ?2-integrin-dependent recruitment in lung inflammation J Leukoc Biol, 2016;0(0):. 2016 [PMID: 27566832] (Flow,, Mouse) Flow Mouse
Rac GTPase Activating Protein ARHGAP25 Regulates Leukocyte Transendothelial Migration in Mice J Immunol, 2016;0(0):. 2016 [PMID: 27566826] (Bioassay, Mouse) Bioassay Mouse
Velazquez F, Grodecki-Pena A, Knapp A, Salvador A, Nevers T, Croce K, Alcaide P CD43 Functions as an E-Selectin Ligand for Th17 Cells In Vitro and Is Required for Rolling on the Vascular Endothelium and Th17 Cell Recruitment during Inflammation In Vivo. J Immunol, 2016;196(3):1305-16. 2016 [PMID: 26700769] (Bioassay, Mouse) Bioassay Mouse
Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, Wang X, Hanjaya-Putra D, Sun L, Morikis V, Simon S, Woods R, Cummings R, Chaikof E Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo. Nat Commun, 2015;6(0):6387. 2015 [PMID: 25824568] (Bioassay, Mouse) Bioassay Mouse
Abdi R, Moore R, Sakai S, Donnelly C, Mounayar M, Sackstein R HCELL Expression on Murine MSC Licenses Pancreatotropism and Confers Durable Reversal of Autoimmune Diabetes in NOD Mice. Stem Cells, 2015;33(5):1523-31. 2015 [PMID: 25641589] (Bioassay, Mouse) Bioassay Mouse
Germena G, Volmering S, Sohlbach C, Zarbock A Mutation in the CD45 inhibitory wedge modulates integrin activation and leukocyte recruitment during inflammation. J Immunol, 2015;194(2):728-38. 2015 [PMID: 25505282] (Bioassay, Mouse) Bioassay Mouse
Show All 29 Publications.

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