Recombinant Mouse DDR1 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse DDR1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind Recombinant Mouse DDR1 Fc Chimera with an apparent K_D < 20 nM.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse DDR1 protein
  

  Mouse DDR1 (Met1 - Thr414) Accession # Q03146	IEGRMDP	Mouse IgG2A (Glu98 - Lys330)
  N-terminus		C-terminus

• Accession #: Q03146
• N-terminal Sequence: Asp22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 71.1 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-95 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse DDR1 Fc Chimera Protein, CF

• CAK
• CD167 antigen-like family member A
• CD167a antigen
• CD167a
• DDR
• DDR1
• discoidin domain receptor family, member 1
• discoidin domain receptor tyrosine kinase 1
• Discoidin receptor tyrosine kinase
• EC 2.7.10
• EC 2.7.10.1
• EDDR1
• ENTRK4
• Epithelial discoidin domain receptor 1
• HGK2
• MCK10
• MCK-10
• NTRK4
• Protein-tyrosine kinase 3A
• PTK3A protein tyrosine kinase 3A
• PTK3A
• receptor, type 4
• RTK6
• TRK E
• TrkE

Background

DDR1, also known as CAK, NEP, CD167a, and EDDR1, is a 120 - 140 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain‑containing subfamily of receptor tyrosine kinases (1, 2). Mature mouse DDR1 consists of a 395 aa extracellular domain (ECD) that includes the discoidin-like domain, a 27 aa transmembrane segment, and a 470 aa cytoplasmic domain with the tyrosine kinase domain (3). Within the ECD, mouse DDR1 shares 93% aa sequence identity with human and rat DDR1, respectively. Alternate splicing of mouse DDR1 generates an additional isoform that lacks a portion of the cytoplasmic domain encompassing the Shc-interacting NPxY motif (4). DDR1 is expressed on epithelial tissues, activated monocytes and neutrophils, smooth muscle cells, and in several cancers (1, 2). It mediates cellular adhesion and migration through interaction of the discoidin-like domain with the triple helical structure of collagens I ‑ V (5, 6). Collagen binding induces prolonged tyrosine autophosphorylation of DDR1, including within the NPxY motif (5, 6). Collagen binding can also induce the metalloproteinase-dependent cleavage of DDR1, thereby liberating a tyrosine phosphorylated 60 kDa C-terminal fragment and a 60 kDa ECD fragment (7, 8). DDR1 is expressed as a dimer on the cell surface independently of ligand binding (9). Oligomerization enhances collagen binding and also modulates collagen fibrillogenesis (10, 11). Collagen-induced activation of DDR1 is inhibited by the association of DDR1 with E-Cadherin at epithelial cell junctions (12). Expression of DDR1 on arterial smooth muscle cells limits smooth muscle cell migration and the development of proteoglycan plaques during atherogenesis (13). The overexpression of particular DDR1 isoforms in glioblastoma promotes MMP-2 activation and increased tumor cell invasiveness (14).

1. Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
2. Yoshimura, T. et al. (2005) Immunol. Res. 31:219.
3. Zerlin, M. et al. (1993) Oncogene 8:2731.
4. Perez, J.L. et al. (1996) Oncogene 12:1469.
5. Shrivastava, A. et al. (1997) Mol. Cell 1:25.
6. Vogel, W. et al. (1997) Mol. Cell 1:13.
7. Slack, B.E. et al. (2006) J. Cell Biochem. 98:672.
8. Vogel, W.F. et al. (2001) FEBS Lett. 514:175.
9. Noordeen, N.A. et al. (2006) J. Biol. Chem. 281:22744.
10. Leitinger, B. (2003) J. Biol. Chem. 278:16761.
11. Agarwal, G. et al. (2007) J. Mol. Biol. 367:443.
12. Wang, C.Z et al. (2009) Am. J. Physiol. Cell Physiol. 297:C419.
13. Franco, C. et al. (2010) Circ. Res. 106:1775.
14. Ram, R. et al. (2006) J. Neurooncol. 76:239.

Publications for DDR1 (6416-DR)(1)

Publication using 6416-DR

• Ria Aryani Hayuningtyas, Myeonggil Han, Seoyeon Choi, Man Sup Kwak, In Ho Park, Ji-Hyun Lee et al. The collagen structure of C1q induces wound healing by engaging discoidin domain receptor 2 Molecular Medicine 2021-12-01 [PMID: 34602056] (Dot Blot Assay, N/A)

FAQs for DDR1 (6416-DR). (Showing 1 - 1 of 1 FAQ).

1. I was wondering if you offer, or are planning on offering, any antibodies targeted to specific phosphorylation sites on the receptor tyrosine kinase DDR1.
   • At this time we do not offer and DDR1 antibodies specific for certain phosphorylation states.

Bioinformatics

• Uniprot:
  • Q03146()