Recombinant Mouse CXCL4/PF4 Protein, CF


Recombinant Mouse CXCL4/PF4 (Catalog # 595-P4) inhibits the FGF basic-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 2-10 μg/mL.
1 μg/lane of Recombinant Mouse CXCL4/PF4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 10 kDa.

Product Details

Reactivity MuSpecies Glossary
Applications Binding Activity

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Recombinant Mouse CXCL4/PF4 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the FGF basic-dependent proliferation of HUVEC human umbilical vein endothelial cells. Dubrac, A. et al. (2010) Blood 116:4703. The ED50 for this effect is 2-10 μg/mL.
E. coli-derived mouse CXCL4/PF4 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Binding Activity
Theoretical MW
8.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
595-P4 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CXCL4/PF4 Protein, CF

  • chemokine (C-X-C motif) ligand 4
  • C-X-C motif chemokine 4
  • CXCL4
  • CXCL4iroplact
  • Iroplact
  • MGC138298
  • Oncostatin-A
  • PF4
  • PF-4
  • platelet factor 4
  • SCYB4oncostatin-A


CXCL4, also called PF4 (platelet factor 4), is an 8 kDa member of the CXC chemokine family, sharing features with CXCL8/IL-8 and CXCL7/NAP-2 (1-3). Mature mouse CXCL4 shares 76%, 88%, 64%, 64% and 63% amino acid sequence identity with human, rat, ovine, porcine and bovine CXCL4, respectively. The active protein is a tetramer of CXCL4 subunits that forms a ring of heparin-binding positive charges from sites at the C-terminal region of each monomer (3). Megakaryocytes synthesize CXCL4 and store it in platelet alpha -granules (2, 3). Secretion from activated platelets can produce micromolar levels in serum and over 100‑fold higher within clots (2, 3). In contrast to other CXC chemokines, CXCL4 does not contain an ELR motif and lacks binding to nearly all chemokine receptors (2, 3). A potential high-affinity G-protein-coupled receptor for CXCL4, the CXCR3 isoform CXCR3B, is expressed in human but not mouse (2, 3). In most cases, it is likely that cell surface binding and signaling properties of CXCL4 are due to binding of glycosaminoglycans chains, particularly chondroitin sulfates (2). CXCL4 released from activated platelets binds and regulates thrombin/thrombomodulin complexes, regulates and enhances production of activated Protein C (APC), and limits the coagulation cascade (2-6). It binds and influences the enzymatic activity of coagulation factor Xa (7). It binds fibrin and affects clot structure (8). Therapeutic doses of the anticoagulant heparin neutralize CXCL4 procoagulant effects (9). The complex between heparin and CXCL4 can be immunogenic, and circulating CXCL4-heparin antibodies cause the human syndrome HITT (heparin-induced thrombocytopenia and thrombosis, also called HIT) (2). In addition, immunogenic complexes of CXCL4 with apolipoprotein H can contribute to antiphospholipid syndrome (APS) (10). CXCL4 can be antiproliferative and antiangiogenic, at least in part via interfering with FGF-2 and VEGF heparin binding and thus inhibiting their signaling (3, 11-13). However, it can also be proinflammatory and pro‑atherogenic through multiple effects on monocytes, macrophages and endothelial cells (2, 3).

  1. Poncz, M. et al. (1987) Blood 69:219.
  2. Kowalska, M.A. et al. (2010) Thromb. Res. 125:292.
  3. Slungaard, A. (2005) Int. J. Biochem. Cell Biol. 37:1162.
  4. Slungaard, A. et al. (2003) Blood 102:146.
  5. Kowalska, M.A. et al. (2007) Blood 110:1903.
  6. Preston, R.J.S. et al. (2009) J. Biol. Chem. 284:5869.
  7. Fiore, M.M. and I.J. Mackie (2009) Biochem. Biophys. Res. Commun. 379:1072.
  8. Amelot, A.A. et al. (2007) J. Biol. Chem. 282:710.
  9. Eslin, D.E. et al. (2004) Blood 104:3173.
  10. Sikara, M.P. et al. (2010) Blood 115:713.
  11. Perollet, C. et al. (1998) Blood 91:3289.
  12. Gengrinovitch, S. et al. (1995) Journal of Biological Chemistry 270:15059.
  13. Sulpice, E. et al. (2004) Eur. J. Biochem. 271:3310.

Publications for CXCL4/PF4 (595-P4)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Binding Assay, Bioassay, In Vivo.

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Showing Publications 1 - 6 of 6.
Publications using 595-P4 Applications Species
Graca, FA;Stephan, A;Minden-Birkenmaier, BA;Shirinifard, A;Wang, YD;Demontis, F;Labelle, M; Platelet-derived chemokines promote skeletal muscle regeneration by guiding neutrophil recruitment to injured muscles Nature communications 2023-05-22 [PMID: 37217480] (Bioassay, Mouse) Bioassay Mouse
S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity Front Microbiol, 2019-06-25;10(0):1421. 2019-06-25 [PMID: 31293551] (Binding Assay, Mouse) Binding Assay Mouse
HK Drescher, EF Brandt, P Fischer, S Dreschers, RA Schwendene, MA Kowalska, A Canbay, HE Wasmuth, R Weiskirche, C Trautwein, ML Berres, DC Kroy, H Sahin Platelet Factor 4 Attenuates Experimental Acute Liver Injury in Mice Front Physiol, 2019-03-26;10(0):326. 2019-03-26 [PMID: 30971954] (Bioassay, Mouse) Bioassay Mouse
T Kuroishi, K Bando, Y Tanaka, K Shishido, M Kinbara, T Ogawa, K Muramoto, Y Endo, S Sugawara CXCL4 is a novel nickel-binding protein and augments nickel allergy Clin. Exp. Allergy, 2017-04-21;0(0):. 2017-04-21 [PMID: 28319310] (Bioassay, In Vivo, Mouse) Bioassay, In Vivo Mouse
de Jong EK, de Haas AH, Brouwer N, van Weering HR, Hensens M, Bechmann I, Pratley P, Wesseling E, Boddeke HW, Biber K Expression of CXCL4 in microglia in vitro and in vivo and its possible signaling through CXCR3. J. Neurochem., 2008-02-01;105(5):1726-36. 2008-02-01 [PMID: 18248618] (Bioassay, Mouse) Bioassay Mouse
Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y The role of CCL21 in recruitment of T-precursor cells to fetal thymi. Blood, 2004-09-09;105(1):31-9. 2004-09-09 [PMID: 15358618] (Bioassay, Mouse) Bioassay Mouse

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Gene Symbol Pf4