Recombinant Mouse CXCL2/MIP-2 Protein


There are currently no images for CXCL2/GRO beta/MIP-2/CINC-3 (452-M2).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Mouse CXCL2/MIP-2 Protein Summary

Details of Functionality
Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474. The ED50 for this effect is 0.15‑0.3 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 0.1‑0.5 ng/mL.
E. coli-derived mouse CXCL2/GRO beta/MIP-2/CINC-3 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
452-M2 in the following application:

Read Publications using
452-M2 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CXCL2/MIP-2 Protein

  • chemokine (C-X-C motif) ligand 2
  • CINC-2a
  • CINC3
  • CINC-3
  • C-X-C Motif Chemokine 2
  • CXCL2
  • GRO beta
  • GRO2 oncogene
  • GRO2
  • GROB
  • Gro-beta
  • Growth-regulated protein beta
  • Macrophage inflammatory protein 2-alpha
  • melanoma growth stimulatory activity beta
  • MGSA beta
  • MGSA-b
  • MGSA-beta
  • MIP2
  • MIP-2
  • MIP2A
  • MIP-2a
  • MIP2-alpha
  • SCYB2


Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines.

MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs.

Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, Simple Western, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, S-ELISA
Species: Hu, Mu
Applications: WB, Simple Western, IHC, ICC
Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
Species: Hu, Mu, Bv, Ca, Pm, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, MiAr, CyTOF-ready, Flow-IC
Species: Hu
Species: Hu, Mu, Rt, Po, Bv, Ma
Applications: WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, PAGE, B/N, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), Flow-CS, Flow-IC, KD, KO
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IF
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

Publications for CXCL2/GRO beta/MIP-2/CINC-3 (452-M2)(45)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 5 applications: Binding Assay, Bioassay, ELISA (Standard), ELISA Development, In Vivo.

Filter By Application
Binding Assay
ELISA (Standard)
ELISA Development
In Vivo
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using 452-M2 Applications Species
JM Adrover, A Aroca-Crev, G Crainiciuc, F Ostos, Y Rojas-Vega, A Rubio-Ponc, C Cilloniz, E Bonzón-Kul, E Calvo, D Rico, MA Moro, C Weber, I Lizasoaín, A Torres, J Ruiz-Cabel, J Vázquez, A Hidalgo Programmed &#039;disarming&#039; of the neutrophil proteome reduces the magnitude of inflammation Nat. Immunol., 2020;21(2):135-144. 2020 [PMID: 31932813] (Bioassay, Mouse) Bioassay Mouse
S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity Front Microbiol, 2019;10(0):1421. 2019 [PMID: 31293551] (Binding Assay, Mouse) Binding Assay Mouse
ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling J. Clin. Invest., 2019;130(0):2555-2570. 2019 [PMID: 31107242] (Bioassay, Mouse) Bioassay Mouse
W Li, G Feng, JM Gauthier, I Lokshina, R Higashikub, S Evans, X Liu, A Hassan, S Tanaka, M Cicka, HM Hsiao, D Ruiz-Perez, A Bredemeyer, RW Gross, DL Mann, YY Tyurina, AE Gelman, VE Kagan, A Linkermann, KJ Lavine, D Kreisel Ferroptotic cell death and TLR4/Trif signaling initiate neutrophil recruitment after heart transplantation J. Clin. Invest., 2019;130(0):. 2019 [PMID: 30830879] (Bioassay, Mouse) Bioassay Mouse
VY Su, KY Yang, SH Chiou, NJ Chen, MH Mo, CS Lin, CT Wang Induced Pluripotent Stem Cells Regulate TREM-1 Expression and the p38 MAPK Pathway in Endotoxin-Induced Acute Lung Injury Stem Cells, 2019;0(0):. 2019 [PMID: 30681755] (Bioassay, Mouse) Bioassay Mouse
M Hossain, E Omran, N Xu, L Liu The Specific Mitogen- and Stress-Activated Protein Kinase MSK1 Inhibitor SB-747651A Modulates Chemokine-Induced Neutrophil Recruitment Int J Mol Sci, 2017;18(10):. 2017 [PMID: 29039777] (In Vivo, Mouse) In Vivo Mouse
Clara Jana-Lui Busch Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice Hepatology, 2016;0(0):. 2016 [PMID: 27981604] (ELISA (Standard)) ELISA (Standard)
Pedro Luiz Rosalen Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process Phytomedicine, 2016;23(13):1583-1590. 2016 [PMID: 27823622] (Bioassay, Mouse) Bioassay Mouse
Hossain M, Qadri S, Xu N, Su Y, Cayabyab F, Heit B, Liu L Endothelial LSP1 Modulates Extravascular Neutrophil Chemotaxis by Regulating Nonhematopoietic Vascular PECAM-1 Expression. J Immunol, 2015;195(5):2408-16. 2015 [PMID: 26238489] (In Vivo, Mouse) In Vivo Mouse
Spahn J, Li W, Bribriesco A, Liu J, Shen H, Ibricevic A, Pan J, Zinselmeyer B, Brody S, Goldstein D, Krupnick A, Gelman A, Miller M, Kreisel D DAP12 expression in lung macrophages mediates ischemia/reperfusion injury by promoting neutrophil extravasation. J Immunol, 2015;194(8):4039-48. 2015 [PMID: 25762783] (In Vivo, Mouse) In Vivo Mouse
Show All 45 Publications.

Review for CXCL2/GRO beta/MIP-2/CINC-3 (452-M2) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using 452-M2:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
 CXCL2/GRO beta/MIP-2/CINC-3 452-M2
reviewed by:
James Xiao




CommentsA total of 10^6 mouse neutrophils was added to an insert of a transwell. Recombinant mouse MIP-2 (100 ng/ml) was used as a neutrophil chemoattractant and was added to the lower chamber. After 2 hours of incubation, migrated neutrophils in the lower chambers were enumerated.

FAQs for CXCL2/GRO beta/MIP-2/CINC-3 (452-M2) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional CXCL2/GRO beta/MIP-2/CINC-3 Products

Bioinformatics Tool for CXCL2/GRO beta/MIP-2/CINC-3 (452-M2)

Discover related pathways, diseases and genes to CXCL2/GRO beta/MIP-2/CINC-3 (452-M2). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CXCL2/GRO beta/MIP-2/CINC-3 (452-M2)

Discover more about diseases related to CXCL2/GRO beta/MIP-2/CINC-3 (452-M2).

Pathways for CXCL2/GRO beta/MIP-2/CINC-3 (452-M2)

View related products by pathway.

PTMs for CXCL2/GRO beta/MIP-2/CINC-3 (452-M2)

Learn more about PTMs related to CXCL2/GRO beta/MIP-2/CINC-3 (452-M2).

Blogs on CXCL2/GRO beta/MIP-2/CINC-3

There are no specific blogs for CXCL2/GRO beta/MIP-2/CINC-3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Recent Reviews


James Xiao


Gene Symbol Cxcl2