Recombinant Mouse CXCL2/MIP-2 Protein

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Summary
Reactivity MuSpecies Glossary
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Recombinant Mouse CXCL2/MIP-2 Protein Summary

Details of Functionality
Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474. The ED50 for this effect is 0.15‑0.3 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 0.1‑0.5 ng/mL.
Source
E. coli-derived mouse CXCL2/GRO beta/MIP-2/CINC-3 protein
Ala28-Asn100
Accession #
N-terminal Sequence
Ala28
Protein/Peptide Type
Recombinant Proteins
Gene
Cxcl2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
452-M2 in the following application:

Publications
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452-M2 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CXCL2/MIP-2 Protein

  • chemokine (C-X-C motif) ligand 2
  • CINC-2a
  • CINC3
  • CINC-3
  • C-X-C Motif Chemokine 2
  • CXCL2
  • GRO beta
  • GRO2 oncogene
  • GRO2
  • GROB
  • Gro-beta
  • Growth-regulated protein beta
  • Macrophage inflammatory protein 2-alpha
  • melanoma growth stimulatory activity beta
  • MGSA beta
  • MGSA-b
  • MGSA-beta
  • MIP2
  • MIP-2
  • MIP2A
  • MIP-2a
  • MIP2-alpha
  • SCYB2

Background

Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines.

MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs.

Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

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Publications for CXCL2/GRO beta/MIP-2/CINC-3 (452-M2)(42)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 5 applications: Binding Assay, Bioassay, ELISA Development, ELISA Standard, In Vivo.


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Binding Assay
(1)
Bioassay
(16)
ELISA Development
(1)
ELISA Standard
(10)
In Vivo
(11)
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Filter By Species
Human
(1)
Mouse
(28)
Rat
(1)
N/A
(8)
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Showing Publications 1 - 10 of 42. Show All 42 Publications.
Publications using 452-M2 Applications Species
W Li, G Feng, JM Gauthier, I Lokshina, R Higashikub, S Evans, X Liu, A Hassan, S Tanaka, M Cicka, HM Hsiao, D Ruiz-Perez, A Bredemeyer, RW Gross, DL Mann, YY Tyurina, AE Gelman, VE Kagan, A Linkermann, KJ Lavine, D Kreisel Ferroptotic cell death and TLR4/Trif signaling initiate neutrophil recruitment after heart transplantation J. Clin. Invest., 2019;130(0):. 2019 [PMID: 30830879] (Bioassay, Mouse) Bioassay Mouse
VY Su, KY Yang, SH Chiou, NJ Chen, MH Mo, CS Lin, CT Wang Induced Pluripotent Stem Cells Regulate TREM-1 Expression and the p38 MAPK Pathway in Endotoxin-Induced Acute Lung Injury Stem Cells, 2019;0(0):. 2019 [PMID: 30681755] (Bioassay, Mouse) Bioassay Mouse
M Hossain, E Omran, N Xu, L Liu The Specific Mitogen- and Stress-Activated Protein Kinase MSK1 Inhibitor SB-747651A Modulates Chemokine-Induced Neutrophil Recruitment Int J Mol Sci, 2017;18(10):. 2017 [PMID: 29039777] (In Vivo, Mouse) In Vivo Mouse
Clara Jana-Lui Busch Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice Hepatology, 2016;0(0):. 2016 [PMID: 27981604] (ELISA Standard) ELISA Standard
Pedro Luiz Rosalen Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process Phytomedicine, 2016;23(13):1583-1590. 2016 [PMID: 27823622] (Bioassay, Mouse) Bioassay Mouse
Hossain M, Qadri S, Xu N, Su Y, Cayabyab F, Heit B, Liu L Endothelial LSP1 Modulates Extravascular Neutrophil Chemotaxis by Regulating Nonhematopoietic Vascular PECAM-1 Expression. J Immunol, 2015;195(5):2408-16. 2015 [PMID: 26238489] (In Vivo, Mouse) In Vivo Mouse
Spahn J, Li W, Bribriesco A, Liu J, Shen H, Ibricevic A, Pan J, Zinselmeyer B, Brody S, Goldstein D, Krupnick A, Gelman A, Miller M, Kreisel D DAP12 expression in lung macrophages mediates ischemia/reperfusion injury by promoting neutrophil extravasation. J Immunol, 2015;194(8):4039-48. 2015 [PMID: 25762783] (In Vivo, Mouse) In Vivo Mouse
Germena G, Volmering S, Sohlbach C, Zarbock A Mutation in the CD45 inhibitory wedge modulates integrin activation and leukocyte recruitment during inflammation. J Immunol, 2015;194(2):728-38. 2015 [PMID: 25505282] (Bioassay, Mouse) Bioassay Mouse
Li S, Pettersson U, Hoorelbeke B, Kolaczkowska E, Schelfhout K, Martens E, Kubes P, Van Damme J, Phillipson M, Opdenakker G Interference with glycosaminoglycan-chemokine interactions with a probe to alter leukocyte recruitment and inflammation in vivo. PLoS ONE, 2014;9(8):e104107. 2014 [PMID: 25093679] (Bioassay, Mouse) Bioassay Mouse
Sonego F, Castanheira F, Czaikoski P, Kanashiro A, Souto F, Franca R, Nascimento D, Freitas A, Spiller F, Cunha L, Zamboni D, Alves-Filho J, Cunha F MyD88-, but not Nod1- and/or Nod2-deficient mice, show increased susceptibility to polymicrobial sepsis due to impaired local inflammatory response. PLoS ONE, 2014;9(8):e103734. 2014 [PMID: 25084278] (In Vivo, Mouse) In Vivo Mouse
Show All 42 Publications.

Review for CXCL2/GRO beta/MIP-2/CINC-3 (452-M2) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using 452-M2:
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 CXCL2/GRO beta/MIP-2/CINC-3 452-M2
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reviewed by:
Jim Hsiao
04/17/2018
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Summary

LotPF0314092

Comments

CommentsA total of 10^6 mouse neutrophils was added to an insert of a transwell. Recombinant mouse MIP-2 (100 ng/ml) was used as a neutrophil chemoattractant and was added to the lower chamber. After 2 hours of incubation, migrated neutrophils in the lower chambers were enumerated.

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Jim Hsiao
04/17/2018
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Gene Symbol Cxcl2
Uniprot